- Research Article
- 10.36560/19320262198
Antifungal activity of Cenostigma pluviosum var. peltophoroides extract against Colletotrichum isolates associated with soybean anthracnose: effects on sporulation, conidial germination, and appressorium formation
- Apr 13, 2026
- Scientific Electronic Archives
- Airton Lima + 7 more +7
Plant-derived compounds have been widely investigated as potential alternatives to synthetic fungicides in plant disease management. This study evaluated the antifungal activity of an ethanolic extract obtained from leaves of Cenostigma pluviosum var. peltophoroides against Colletotrichum spp. isolated from soybean plants affected by anthracnose. The isolates were obtained from soybean fields located in three municipalities in the state of Mato Grosso, Brazil. Antifungal activity was evaluated using in vitro assays, including disk-diffusion and spreading methods, to assess mycelial growth inhibition, mycelial growth rate index, sporulation inhibition, conidial germination, and appressorium formation. The extract showed limited inhibition of mycelial growth but significantly reduced sporulation and strongly inhibited appressorium formation, reaching up to 92.30% inhibition. The spreading method showed greater sensitivity for detecting sporulation inhibition compared with the disk-diffusion method. These results indicate that the extract interferes with important stages of the fungal infection process and may represent a potential source of bioactive compounds for alternative management strategies for soybean anthracnose. Further studies are required to identify the active compounds and evaluate their effectiveness under greenhouse and field conditions.
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