Implantology focuses on the measurement of bone thickness in both the lower and upper jaws. This study aimed to measure and compare alveolar bone thickness of the upper and lower jaws at single edentate sites and cortical bone thickness of their mesial and distal dentate sites. Thickness of alveolar bone thickness was measured in 80 upper and 80 lower implant edentate sites and that of buccal and lingual cortical plates of their mesial and distal dentate sites using Cone beam CT. The bone thickness of the edentulous sites was recorded at 3 points (crestal bone, five mm from the crest, and ten mm from the crest), while the bone thickness of the dentate sites was determined at four points (crestal bone, midroot bone, mid of the alveolar bone housing, and apical portion). An increased amount of bone was measured from the crest to the apical portion of the dentate sites on the buccal and lingual sides of both jaws with a highly significant difference detected among all the tested points (P < 0.0001). No statistical difference was detected between the means of buccal bone width at the first 3 points, except at point 4 (the apical portion), where the mean of the lower jaw (3.35 ± 0.54) was significantly larger than that of the upper jaw (3.17 ± 0.55) (P = 0.04). Bone width measured in the edentulous sites showed a gradual increase from the crest to the apical portions in both jaws. Bone thickness at the coronal levels is low and susceptible to resorption compared to the apical portions regardless of the dentate state.

The production of nonindustrial cannabis is highly developed in the Moroccan Rif region; however, local farmers consider hemp seeds which are rich in omega 3 and tocopherols, only as by-products of cannabis cultivation with low market value. The local ecotype is considered to be a plant with a cannabinoid content of more than 0.4%. So, the objective of this research is to investigate how the incorporation of this local hemp seed affects productive performance and egg quality traits. The experiment is conducted to evaluate the effects of hemp seed (HS) incorporation on hen laying performance and physical egg quality at three levels: 10% (HS-10% group), 20% (HS-20% group), and 30% (HS-30% group). Ninety-six Lohmann Brown classic laying hens were randomly assigned to a control group and three feed treatments. The sampling was taken after the 28-week rearing period (peak egg laying). Throughout the experiment, low-rate HS inclusion (HS-10%) showed no significant differences in egg-laying performance (p > 0.05). However, the high incorporation rates of HS (20% and 30%) negatively affected the egg-laying performance (84–94% and 80–86%, respectively). The albumen quality was also improved by the HS inclusion, where the highest values of the Haugh unit were recorded, ranging between 68.69 and 73.91 for the HS-30% groups. The results also show that HS inclusion and duration influence significantly the yolk color (p < 0.001). The yellow intensity decreases with HS incorporation and aging, from a dark yellow (b∗ = 38.63 for the control group) to a very pale yellow (b∗ = 26.29 for HS 30% group). Based on these findings, we can conclude that the incorporation of nonindustrial Moroccan cannabis seeds (ecotype Beldiya) at low rate in the diet of laying hens does not alter the laying performance or the quality of the egg; therefore, they could be used in poultry feeding as an alternative constituent to partially replace high-cost imported ingredients, such as corn and soybeans.

Our current study was conducted in Zijje Maryam Church Forest, Ethiopia, to explore woody species composition, structure, regeneration status, and anthropogenic disturbances inside the sacred groves. The aforementioned information for adequate conservation and management of the church forest is not well documented. Fifteen main quadrats each having an area of 625 m2 (25 m × 25 m) were used for vegetation and disturbance data collection. Determination of the sampled quadrats was based on the principle that minimum quadrats give the smallest possible area in which all species occurring in the church forest are present. All woody species with a diameter at breast height (DBH) ≥ 2.5 cm within the quadrat were identified, counted, and their height and DBH data were recorded. The criterion to start at DBH ≥ 2.5 cm was to exclude seedlings having DBH < 2.5 cm and height ≤0.6 m. Sapling and seedling data were collected using 45 saplings and 45 seedling quadrat that measured 4 m2 and 1 m2, respectively. Vegetation data analysis and ANOVA were used for statistical comparison. A total of 48 woody plant species belonging to 46 genera and 36 families were identified. Fabaceae was the dominant family containing 5 species followed by Rosaceae with 3 species. Total basal area of the church forest was 83.03 m2 ha−1. The density of seedlings, saplings, and matured woody species stem ha−1 were 15555, 3833, and 865, respectively. Talking these densities, the regeneration status of the forest was good. The Shannon diversity and evenness of woody plant species in the forest was high, 3.29 and 0.85, respectively. Juniperus procera 27.67 (9.22%) and Olea europaea were species with the highest IVI. Nearly, 22% of areas of the forest get disturbed and higher anthropogenic disturbances occurred near the edge of the forest. Gathering, clearing, and grazing are the major human disturbances that stakeholders need to tackle for conservation. Zijje Maryam Church Forest has heterogeneous species composition with varied seedlings and saplings. Therefore, local conservation policies recommended not only protect large forests, but also the small and valuable forests service to the needs of local people.

Objective Suicidal behavior among adolescents is a major public health problem that is understudied in South East Asian Muslim-majority countries. We aimed to investigate the rate and associated factors of suicidal behavior among adolescents in Bangladesh and Indonesia. Methods The Global School-based Student Health Survey data of Bangladesh and Indonesia were used in this study. The data consist of a total of 9052 school-aged students from Bangladesh (2570, 28.4%) and Indonesia (6482, 71.6%). Suicidal behavior was assessed using three questions that measure suicidal ideation, suicidal plan, and suicidal attempts. Results The overall rate of suicidal behavior (suicidal ideation, suicidal plan, and suicidal attempts) was 8.8%, and no significant difference between the two countries (8.9% in Bangladesh and 8.7 in Indonesia) was observed (p=0.81). Factors that independently increased the likelihood of suicidal behavior include female gender, missed class, physical fight four times or more, experienced bullying, anxiety, loneliness, rarely eating fruit, current alcohol use, and sedentary behavior (p < 0.05). Meanwhile, factors that independently decreased the likelihood of suicidal behavior include parental supervision and having close friends, either one, two, three persons, or more (p < 0.05). Conclusion The study revealed rates and risk factors of suicidal behaviors among the school-going adolescents of two Muslim-majority countries in South East Asia. Prevention strategies should be considered guided by the risk factors for school-going adolescents.

In line with the increase in population, the production of waste will also grow every year. Unfortunately, the land used for final waste disposal (landfill) is extremely limited, especially in developing countries. This study aimed to predict landfill capacity in accommodating waste from the community based on daily waste input using a dynamic system and to provide alternative policies on landfill waste management. The analyzed data consisted of primary and secondary data, whereas the simulations applied system dynamic approach using Vensim software. The simulation results indicate that the waste production will reach 36,861,653 tons in 2030 if nothing is done. Assuming that all waste from the surrounding communities is accumulated in one landfill, Bantargebang landfill can only accommodate until 2022. To be able to use Bantargebang landfill up to 2030, the waste production must be reduced by 50% for organic waste and 50% for inorganic waste. From the analysis, it is proven that composting reduces a greater amount of waste than other waste reduction methods, namely recycling or reusing, by the same percentage. Waste sorting is therefore highly recommended to be done by the community in every household as a basis to facilitate further handling. The community can play an active role in reducing waste, e.g., by composting organic waste and recycling or reusing inorganic waste. Furthermore, regulations should be made that can give punishment to households that do not carry out segregation. Reliable infrastructure for waste management needs to be facilitated, and counseling/training/outreach on waste sorting to the community must also be provided at the district level.

This study aims to evaluate the potency of ethanol extract of red okra pods (EEROP) in inhibiting growth of cervical cancer cells through repression of the cell cycle-associated oncogenes. The EEROP treatment was given to HeLa cells cultured with RPMI medium and incubated at 37°C with 5% CO2. The MTT method was used to measure HeLa cell growth and IC50 values. The mRNA levels of the three cell cycle-associated oncogenes (MYC, TYMS, and MDM2) were evaluated by qRT-PCR to determine the effect of EEROP treatment on the cell cycle. The lowest percentage of viable cells at 24, 48, and 72 hours after EEROP treatment was in the dose of 1000 μg/mL with a growth percentage of 71.60% at 24 hours, 55.61% at 48 hours, and 46.97% at 72 hours. The IC50 values were 2845, 1153, and 776.8 μg/mL for 24, 48, and 72 hours, respectively. The three oncogenes at a dose of 1000 μg/mL significantly decreased the lowest mRNA levels compared to other doses with MYC oncogene that experienced the greatest decrease. The mRNA level of dose 1000 μg/mL EEROP at the MYC oncogene was 0.014-fold changes, at the TYMS oncogene was 0.097-fold changes, and at the MDM2 oncogene was 0.028-fold changes. The EEROP has been shown to decrease the expression of three cell cycle-associated oncogenes. This is also supported by the growth of HeLa cells that did not increase throughout 24, 48, and 72 hours. However, further research is needed on the main active components in red okra that function as anticancer, so that in the future, okra can not only stop cancer cell growth but also induce cancer cell death.

In the terrestrial ecosystem, vegetation is the important component of exchanging of water and energy in biogeochemical and climate cycle. A study was conducted to detect the vegetation cover change at Az Zakhnuniyah island by using remote sensing techniques. It includes vegetation analysis using normalized difference vegetation index (NDVI) while comparing with climatological data including temperature, humidity, and precipitation. A clear trend was seen in climatological parameters where temperature and humidity were rising decade by decade although NDVI did not show. In addition, increasing soil salinization over the years was observed when soil salinity index was used. NDVI-based long-term decadal analysis on vegetation cover based on Landsat surface reflectance data showed increase of vegetation cover which was also linked to precipitation trends. Also, the short-term demi-decadal comparison using PROBA-V showed the vegetation cover reduction between 2015 and 2019. Nevertheless, the sea level surrounding the island also showed an increasing trend of 0.34 cm/y, which could be the cause of inundation in some parts of the island in future. Furthermore, all these trends need to be observed in entirety as many of those trends can be interlinked.

Introduction Hypoxic-ischemic encephalopathy (HIE) is defined as a neurological complication that results from perinatal asphyxia. Previous studies had investigated various markers to early detect HIE; however, these markers appeared to have several drawbacks, especially in resource-limited settings. Aim This study aimed at evaluating the predictive value of the salivary lactate dehydrogenase level as a potential predictor of hypoxic-ischemic encephalopathy for newborns. Materials and Methods We included 30 neonates with HIE due to perinatal asphyxia and 30 healthy newborns that serve as controls, admitted at the intensive care unit for neonates and maternity ward at Ismailia area Clinics and Hospitals. We measured the LDH levels by using saliva samples that were collected for neonates maximum by 12 h after birth. Results It was found that patients with HIE had a statistically significant higher salivary LDH level (1927 ± 390.3 IU/L) than patients without HIE (523.6 ± 142.8 IU/L) (p < 0.001). Moreover, salivary LDH showed a good discriminative ability where the AUC was 0.966 regarding salivary LDH (95% CI: 0.917–1.0) (p < 0.001). The best cutoff value was 1420 IU/L or more which showed the best results in predicting the occurrence of HIE with 98.3% and 97.6% sensitivity and specificity, respectively. Conclusion Salivary LDH can be considered as a useful noninvasive laboratory marker that can accurately predict HIE incidence among neonates with asphyxia within 12 hours from birth. The cases in the HIE group were assigned into three stages according to the Sarnat and Sarnat staging system: stage I: mild (irritable, normal, or hypertonia and poor feeding); stage II: moderate (lethargy, hypotonia, and frequent seizure); stage III: severe (coma, flaccid, absent reflexes, and frequent seizure). There is a positive association between LDH levels and the severity of HIE.

Delayed union and nonunion fractures are clinical challenges for orthopedic surgeons. The development of fracture complications, such as delayed union and nonunion fractures, is still difficult to predict. Various methods are being investigated to improve fracture healing and prevent complications in patients. There are various methods to promote fracture healing, broadly divided into biological, chemical, and physical methods. One of the most widely used physical methods to promote fracture healing is the pulsed electromagnetic field (PEMF). This study aimed to evaluate the healing process of delayed union fracture after being stimulated by PEMF. Twenty-four rats were randomly divided into two groups: the control group (n = 12) and the PEMF group (n = 12). Delayed union fracture was performed on the left femur of all rats. Subsequently, the PEMF group was given PEMF stimulus with a magnetic field intensity of 1.6 mT and a frequency of 50 Hz for 4 hours/day and 7 days/week. The fracture healing process was evaluated on days 5, 10, 18, and 28 based on the bone callus histology using safranin O fast green (SOFG) staining. The results of the histological analysis showed that bone cartilage was higher in the PEMF group than in the control group throughout the observation period. In addition, the PEMF group had less fibrous tissue at the beginning of the healing. This finding indicates PEMF stimulation has an effect on inducing osteogenesis on fracture healing and reducing the risk of delayed union.

Background Although available evidence suggests short birth intervals are associated with adverse perinatal outcomes, little is known about the extent to which birth spacing affects postnatal child growth. The present study assessed the independent association of birth interval with birth weight and subsequent postnatal growth indices. Methods This retrospective cohort study carried out in the rural areas of Kassena-Nankana district of Ghana compared postnatal growth across different categories of birth intervals. Birth intervals were calculated as month difference between consecutive births of a woman. The study population comprised 530 postpartum women who had delivered a live baby in the past 24 months prior to the study. Results Using the analysis of covariance (ANCOVA) that adjusted for age of the child, gender of the child, weight-for-length z-score (WLZ), birth weight, adequacy of antenatal care (ANC) attendance, and dietary diversity of the child, the mean length-for-age z-score (LAZ) among children of short preceding birth interval (<24 months) was significantly higher than among children of long birth interval (that is, at least 24 months) ((0.51 versus −0.04) (95% CI: 0.24–0.87), p = 0.001). The adjusted mean birth weight of children born to mothers of longer birth interval was 74.0 g more than children born to mothers of shorter birth interval (CI: 5.89–142.0, p< 0.03). Conclusions The results suggest that a short birth interval is associated positively with an increased risk of low birth weight (an indicator of foetal growth), but birth spacing is associated negatively with the LAZ (an indicator of postnatal growth).