The genus Kudoa includes about 100 described species. In Vietnam, so far, only one species, Kudoa monodactyli, has been reported. During servey for parasites of marine fish in Quang Binh province, Vietnam in 2017, we found Myxozoan samples in 8 out of 15 Indo-Pacific king mackerel (Scomberomorus guttatus). They are identified as K. scomberomori based on the results of morphological and molecular analyses. A great number of individual spores form white pseudocysts with thin membrane within the muscle fibre. The pseudocysts were 0.1-0.3 × 0.4-0.7 mm. The shape of fresh spore like a flower petal. The spore of K. scomberomori was 6.65 ± 0.09 (6.53-6.76) µm in length, 7.42 ± 0.13 (7.29-7.58) µm in width and 6.55 ± 0.11 (6.40-6.65) µm in thickness. Each spore has six shell valves with same size. Each shell valve contained one polar capsule measuring 3.42 ± 0.09 (3.32-3.48) µm in length and 1.30 ± 0.14 (1.14-1.46) µm in width. This is the first report of K. scomberomori in Vietnam and Indo-Pacific king mackerel is recorded as a new host for this species. Citation: Nguyen Ngoc Chinh, Ha Duy Ngo, Nguyen Huu Duc, Nguyen Thuy Linh, Pham Ngoc Doanh, 2017. Morphological and molecular characteristics of Kudoa scomberomori (Myxosporea: Kudoidae) firstly foundfrom the muscles of info-pacific king mackerel Scomberomorus guttatus in Quang Binh province, Vietnam. Tap chi Sinh hoc, 40(1): x-xx. DOI: 10.15625/0866-7160/v40n1.10671. *Corresponding author: chinhnn89@gmail.com Received 13 September 2017, accepted 2 December 2017

Sonneratia apetala pneumatophore samples were collected from Xuan Thuy National Park, and then 80% methanol extract was fractionated through Sephadex LH-20 column chromatography. After elution with 95% ethanol and 50% acetone, two phenolic fractions were obtained from the crude extract of of S. apetala pneumatophore. The acetone fraction consisted of tannins having high antioxidant activity, 1.14 times higher than that of ascorbic acid. This fraction also have higher activity of α-amylase inhibition (IC50 0.83 mg/mL) and R. sativus root growth inhibition (IC50 6.4 mg/mL) than did the ethanol fraction. In contrast, ethanol fraction consisted of low-molecular phenolic compounds having inhibitory effects on Samonella tiphimurium growth. Such antibacterial activity was not observed in the acetone extract. Citation: Nguyen Sao Mai, Dao Van Tan, 2017. Fractionation of phenolic compounds from Sonneratia apetala pneumatophores and their bioactivities. Tap chi Sinh hoc, 39(4): 451-456. DOI: 10.15625/0866-7160/v39n4.10708.*Corresponding author: daotanvn@yahoo.comReceived 19 September 2017, accepted 12 December 2017

Pseudomonas moraviensis AN-5 isolated from contaminated sludge could utilize aniline as a sole carbon and nitrogen source. The aniline degradation by cells immobilized in alginate, polyvinyl alcohol (PVA) and polyurethane foam (PUF) was significantly better than freely suspended cells. The immobilized AN-5 degraded aniline nearly completely at the initial concentration of 22 mM by 48 hr of incubation. Compared to other binding materials, PUF-immobilized cells were more stable in aniline biodegradation in reused cycles. Moreover, immobilized cells could be stored for three months at 4oC with little reduction of the aniline degradation capacity. These results revealed that PUF-immobilized P. moraviensis AN-5cells has enhanced aniline biodegradation activity. Citation: Ha Danh Duc, Bui Minh Triet, 2017. Biodegradation of aniline by freely suspended and immobilized Pseudomonas moraviensis AN-5. Tap chi Sinh hoc, 39(3): 303-308. DOI: 10.15625/0866-7160/v39n3.9630. *Corresponding author: hadanhduc@gmail.com Received 11 April 2017, accepted 20 August 2017

The present study was carried out to prepare a checklist of butterflies in the Coastal areas of Purba Medinipur District of southern West Bengal between January 2014 and February 2016. A total of 112 butterfly species belonging to 74 genera under 14 subfamilies and five families were recorded during the study period. Nymphalidae and Lycaenidae were the most dominant family, whereas Papilionidae was the least dominant family. Among 112 butterfly species recorded, 9 species were legally protected under the Wildlife (Protection) Act, 1972. A total of 59 species were newly recorded in the study area. Suastus minuta Moore, 1877 was recorded for the first time in West Bengal. The present results also provide a detailed comparison of butterfly diversity among different studies conducted in southern West Bengal. Citation: Payra A., Mishra R. K., Mondal K., 2017. Butterflies (Lepidoptera - Rhopalocera) of coastal areas of Southern West Bengal, India. Tap chi Sinh hoc, 39(3): 276-390. DOI: 10.15625/0866-7160/v39n3.9243. Received 21 February 2017, accepted 10 March 2017 *Corresponding author: arajushpayra@gmail.com

Billolivia cadamensis (Gesneriaceae) is described as a new species from the central province of Quang Ngai, Vietnam. The new taxon is close to B. tichii but differs in shorter stem, abaxially glabrous bracts, calyx divided into 5 lobes to base, outside pubescent apical half of corolla tube, oblong or deltoid corolla lobes, dense hairs on apical 3/4 of the ovary and white corolla lobes. A key to all known species of Billolivia of Vietnam is given. Received 3 November 2016, accepted 27 December 2016, published online 28 December 2016

Hibiscus sagittifolius Kurz is one herb plant with tuber roots used in traditional medicine thanks to numerous pharmaceutical properties, such as brain stimulus, vitality strengthening, anti-neurasthenia, etc. Seed germination rate of H. sagittifolius is very low in nature; therefore, aiming to create the large number of uniform, high quality plants, factors affecting the propagation process including effects of sugar concentration, vitamins, light intensity and culture mineral components were investigated. After 42 days of culture, in vitro leafy nodal cuttings of H. sagittifolius cultured photoautotrophically in polypropylene bags under high light intensity, 150 µmol m-2 s-1, photoperiod of 12 h d-1, room temperature at 25oC ± 2oC and relative humidity (RH) of 55% ± 5%, showed a better growth than that cultured photomixotrophically or photoautotrophically under low light intensity, 75 µmol m-2 s-1. On 6 different culture media (MS, 1/2 MS, 1/2 NH4, SH, B5, EN), in vitro leafy nodal cuttings of H. sagittifolius cultured photoautotrophically on SH medium had the greatest increased fresh weight (384.9 mg/plant), and their root growth conformed to their shoot growth better than those in other treatments on day 42. This study proved that H. sagittifolius plants had the best growth when cultured in vitro in polypropylene bags having two paper membranes, on SH mineral medium without sucrose and vitamins, under light intensity of 150 µmol m-2 s-1, photoperiod of 12 h d-1, room temperature at 25oC ± 2oC and RH of 55% ± 5%. Citation: Nguyen Le Thu Minh, Nguyen Thuy Phuong Duyen, Le Thi Tuyet Anh, Nguyen Thi Quynh, 2017. Effects of sucrose concentration, vitamins, light intensity and mineral components on growth of Hibiscus sagittifolius Kurz cultured in vitro. Tap chi Sinh hoc, 39(1): 86-95. DOI: 10.15625/0866-7160/v39n1.8468. *Corresponding author: qtnguyen_vn@yahoo.com. Received 6 July 2016, accepted 20 March 2017

SSR molecular markers are commonly applicated in breeding, mapping of useful genes, genetic mapping, researching population and linkage genetics. In this study, we analyzed the characteristics of the size of some SSR fragments and evaluated genomic diversity of 18 tea varieties/clones collected from Tan Cuong commune, Song Cau tea Company and Minh Lap commune (Dong Hy district), Thai Nguyen province. Amplification results of SSR fragments with 14 specific primer pairs from research samples are indicated sequence diversity and diversity of the DNA fragments to be amplified. Using NTSYS version 2.1 software to analysis diversity of DNA fragments amplified by PCR-SSR, research varieties/lines tea is divided into two main groups with coefficient of variation between them with the remaining 0.4. Additionally, nucleotide sequences of SSR markers which related gene encoding glyoxalase and sucrose 6-phosphatephosphatase are sequenced and analyzed as a basis for teas breeding. Investigation result indicated repeat motif diferrent when compare research tea samples and submitted sequence. Citation: Hoang Thi Thu Yen, Duong Thi Nhung, Ha Thi Thanh Hoan, Le Bac Viet, Nguyen Huy Hoang, 2017. Investigation SSR marker from Camellia sinensis growing in Thai Nguyen province. Tap chi Sinh hoc, 39(1): 68-79. DOI: 10.15625/0866-7160/v39n1.7594 *Corresponding author: yenhtt@tnus.edu.vn. Received 30 December 2015, accepted 20 March 2017

Plesiocoelus van Achterberg is a rare genus of the subfamily Agathidinae (Braconidae). The genus was established by van Achterberg in 1990 with one Neotropical species included, Plesiocoelus bassiformis van Achterberg, 1990. Based on specimens deposited at the Institute of Ecology & Biological Resources (IEBR) and Vietnam National Museum of Nature (VNMN), the genus Plesiocoelus van Achterberg is newly recorded from Vietnam with a new species, Plesiocoelus vietnamicus Long & van Achterberg, sp. n. described and illustrated in this paper. Differences between the new species and P. bassiformis van Achterberg, are given.

HV1 and HV2, which located in control D-loop region of mitochondrial, are the hyper-variable parts of mitochondrial DNA (mtDNA). Therefore, these two sequences were often used for evolutional and forensic science. In order to determine the genetic variation of populations belong to Nam-A and Nam-Dao language systems, we sequenced and analyzed the mtDNA HV2 region of individuals from four ethic groups: Kinh, Muong, Ede and Jarai which represent for those language systems. Study subjects are peripheral blood of 169 individuals belonging to four populations: Kinh, Muong, Ede and Jarai. HV2 regions were sequenced by Sanger sequencing. As a result, the analyzed mitochondrial control region sequences could be assigned to 79 different haplogroups, with the dominant proportion of three haplogroups: R, B and F. Donors representing Kinh or Muong population revealed higher haplogroup composition variation in comparison with those affiliated with Jarai or Ede. In addition, it was indicated that the 4 populations shared the genetic analogy with other populations inhabited in Southeast Asia and South Asia.

The interest in microbial surfactants has been steadily increasing in recent years, as the microbial surfactants have numerous advantages compared to chemical surfactants, such as a lower toxicity, better environmental compatibility and effective properties at extreme temperature, pH levels and salinity. A highly biosurfactant-producing strain of yeast, 1214-BK14 was selected among the isolated strains from producing oil wells at White Tiger oil field in Vung Tau, Vietnam. The identification using API 20C AUX showed that the 1214-BK14 strain similar 99% with Cadida tropicalis. The suitable condition for the biosurfactant production by the strain 1214-BK14 were found to be 37oC, pH6, 4% and 0,45% (w/v), for temperature, initial solution pH, intitial concentration of carbon substrate (crude oil), and intitial concentration of nitrogen substrate ((NH4)2SO4), respectively, and the emulsification index (E24) measured in the conditions was 71%. The total crude oil and C10 - C43 alkanes degradation efficiency by the strain 1214-BK14 estimated using GC/MS were 83,37% and 74,54-97,46%, respectively. These results revealed that the strain 1214-BK14 exhibited a tremendous potential for contaminated-crude oil degradation and microbial enhanced oil recovery (MEOR).