Drug development continues to be carried out so that drugs can work effectively. Creating a drug formula can be achieved by combining two or more synergistic drug compounds by hybridization, blending, or synthesis. This study reports findings of antioxidant activity using the good DPPH method from the synthesis of eugenol and salicylic acid using the encapsulation technique. The encapsulation technique uses chitosan as a coating material to protect the active ingredients, taking advantage of its unique functional characteristics, including the ability to form bonds with the crosslink agent sodium tripolyphosphate (Na-TPP) at a speed of 13,500 rpm with a pH of 4.2 to optimize the formulation. The encapsulation system produced majority particles measuring 241.0 ± 19.8 nm, with an encapsulation efficiency of 58.29 ± 0.40%. The results of the compound antioxidant test (CS-ES NP) showed better results than pure ES, with an IC50 value of 1.64 ?g/mL. The morphology results after encapsulation showed round and dense particles, indicating that the ES compound was successfully encapsulated.

Star anise (Illicium verum) is widely used as a medicinal herb and spice. The extracts, essential oil and oleoresin, can be produced by hydro-distillation and steam distillation, while solvent and supercritical fluid extraction can be used to extract oleoresin. In this work, the star anise is distilled to obtain the oil, subsequently, its residue is extracted by solvent extraction to get the oleoresin. Whole and grounded star anise fruit is distilled by hydro-distillation for 8, 12, 16, 20, and 24 hours, thus the highest yield is obtained at 20 hours from grounded fruit, and the highest trans-anethole content of 68.50% is obtained at 8 hours from grounded fruit. Residue from the whole fruit-distillation process is extracted by Soxhlet extraction with three types of solvent (ethanol, diethyl ether, and n-hexane) for 6 hours. The highest yield was obtained from ethanol extraction with 1.16% yield and the highest content of trans-anethol was obtained from n-hexane fraction. One sample with the highest content of anethole from whole and grounded fruit-distilled oil and oleoresin are picked, and later they are tested to measure their antioxidant capacity by Ferric Reducing Antioxidant Power Assay (FRAP).

In cultivation of vegetable plants, plant factory system requires flexible approach and resolution methods with a high degree of accuracy, considering the main requirement quality of plant factory is internal environmental conditions that must be a constant value for a long period, while the value of temperature and humidity environment is fluctuate easily. The purpose of this study is to find out the steps of obtaining fuzzy values and knowing the value of PWM (Pulse Width Modulation) output in the plant factory for a set point temperature of 28 0 C and a set point humidity of 75% using the Tsukamoto fuzzy approach. Fuzzy logic is an approach method and problem solving by modeling linear and non-linear functions, the main advantages of this method are being very flexible, high tolerance and high-value precision as well natural language use in this method, so it can be understood easily. Pak choy plants ( Brassica Chinensis L. ) can grow ideally at temperatures of 19-30 o C and 75% humidity. The steps of research is, first determine the input and output variables which are firm sets. Second, fuzzy compositions, are the steps of changing input values to the value of functions involve. Third, inference fuzzy set of Tsukamoto with the minimum method. Fourth, defuzzication with re-convert data in fuzzy set to crisp data. The last step is to get the output value of the defuzzification result which converted to PWM (Pulse Width Modulation) value. The calculation results obtained a PWM (Pulse Width Modulation) value of 760.2450 for the set point temperature of 28 o C and humidity of 75% in the plant factory of pak choy’s cultivation. Keywords : Fuzzy Logic, Fuzzy Tsukamoto, High Accuracy, Pak Choy, Plant Factory, PWM.