Affiliated Zhongshan Hospital of Dalian University

Ovarian tissue cryopreservation (OTC) is essential for fertility preservation in cancer survivors, prepubertal girls, and individuals at high risk of premature ovarian insufficiency (POI). Yet conventional evaluation methods, such as histology and follicle counting, provide limited insight into tissue viability, microenvironmental integrity, molecular injury, and oncologic safety. Recent advances in multi-omics, including transcriptomics, single-cell sequencing, proteomics, and spatial transcriptomics, enable high-resolution characterization of follicular heterogeneity, stromal status, and potential malignant contamination. Concurrently, artificial intelligence (AI) offers automated follicle detection, quantitative tissue assessment, and multimodal prediction models that can support individualized clinical decisions. This review summarizes emerging applications of multi-omics and AI in ovarian tissue quality assessment and highlights their potential to transform fertility preservation strategies. Integrating molecular profiling with AI-based prediction may establish a more precise and intelligent framework for tissue selection, transplantation planning, and reproductive outcome prediction.

Spinal cord injury (SCI) is characterized by its high incidence, high disability rate, and high treatment costs, making the development of effective therapeutic strategies a major focus. Secondary injury mechanisms in SCI involve complex processes such as inflammation, oxidative stress, and apoptosis, which are critical to patient prognosis. This study investigates the role of SIRT1 in SCI repair, particularly its molecular mechanisms in regulating the P53/Nrf2 signaling pathway. By constructing a recombinant adenovirus overexpressing SIRT1 and conducting both in vitro and in vivo validation, we found that SIRT1 modulates the P53/Nrf2 pathway by downregulating P53 and upregulating Nrf2 expression, significantly inhibiting the expression of inflammatory factors TNF-α, IL-1β, and IL-6, while increasing the expression of the anti-inflammatory factor IL-10. Additionally, SIRT1 reduced apoptosis by regulating Bax and Bcl-2 expression, and enhanced cellular antioxidant capacity through activation of Nrf2. These regulatory effects significantly promoted motor function recovery in SCI rats. This study provides mechanistic evidence for the neuroprotective role of SIRT1 in SCI and establishes a foundation for considering SIRT1 as a novel therapeutic target for SCI.

Paligenosis is an emerging model of mature cell plasticity that enables terminally differentiated cells to re-enter the cell cycle and contribute to tissue regeneration following injury. This tightly regulated process involves an initial phase of autophagy-mediated organelle clearance coupled with mechanistic target of rapamycin complex 1 (mTORC1) suppression, followed by mTORC1 reactivation and stemness gene induction that establishes regenerative competence, ultimately culminating in proliferation and lineage restoration. While paligenosis provides an adaptive mechanism to rapidly replenish lost cells in tissues such as the stomach and pancreas, persistent activation under chronic stress or oncogenic signals may drive metaplasia and tumor initiation. Recent evidence further implicates paligenosis-like programs in cancer progression, metastasis, and therapy resistance, underscoring its dual roles in regeneration and disease. Ongoing investigation into the molecular circuitry of paligenosis-including metabolic rewiring, canonical signaling, and epigenetic remodeling-may yield new insights into both tissue repair and oncogenic transformation, offering novel opportunities for therapeutic targeting.