Bartlett Public Library District

Abstract Hylurgus ligniperda (F.) is a highly successful invader among bark beetles (Scolytinae) and forest insects in general. Native to the western Palearctic region, it has become established in every continent where its host plants ( Pinus spp.) occur. Especially in southern hemisphere regions with large pine plantations, it is often highly abundant. As a repeat invader with a wealth of information on various aspects relevant for biological invasions, it is highly suitable as a model organism for studying the role of international trade, the planting of non-native trees, and the potential occurrence of bridgehead invasions (where abundant non-native populations precipitate further invasions). In the present study, our specific objectives were to reconstruct the worldwide invasions of H. ligniperda and the pathways involved by using a multi-pronged approach including population genetics, analysis of historic interception data generated from inspections of imports, and records of establishments in the literature. Our review of the native and non-native ranges of H. ligniperda and the chronology of establishments revealed at least 13 separate invasions of non-native regions, beginning with Madeira (Portugal) before 1850, and, most recently, eastern China in 2019. We compared the population genetics of 464 specimens from eight countries in the native range and eight countries in the non-native range. Sequencing of the mitochondrial COI gene revealed the presence of 29 haplotypes in six well-supported clades, based on a Bayesian analysis. Non-native populations had significantly lower haplotype diversity (mean h = 0.219) than populations in the native range (mean h = 0.691). Countries in the non-native range had an average of about two haplotypes compared with about four haplotypes in native countries. In the non-native range, only one or two haplotypes were dominant, and these differed among invaded regions except for haplotype HL-H3 which occurred in Australia, New Zealand and China as well as in four countries in southern Europe, and HL-H4 which was dominant in New Zealand, California, New York State, and eastern China as well as two countries in southern Europe. Analyses of interceptions of H. ligniperda with imports arriving in five countries revealed that between 74% and 99% of interceptions originated from other non-native regions while in the USA, most interceptions were linked to imports from the native range beginning in the 1970s. Based on the combined evidence of the chronology of invasions, interception data, and analysis of haplotype distribution, we conclude that the early invasions (before 1950) probably all originated from the native range, while several of the more recent invasions probably originated from parts of the non-native range (suggestive of a bridgehead effect). However, it cannot be determined with certainty what the original sources of each of the invading populations were.

Societal Impact StatementTrees in urban environments provide us with shade, heat mitigation, flood abatement, noise and pollution reduction, pollination, beauty, and much more. However, many of these benefits are strongly connected to tree size and vitality, with larger, healthier trees providing ecosystem services more effectively, which means that selecting the right tree for site and function is crucial in order to gain all benefits from our urban trees.SummaryTrees play a major role in the Earth's biogeochemical processes, influencing soil production, hydrological, nutrient and carbon cycles, and the global climate. They store about 50% of the world's terrestrial carbon stocks, and provide habitats for a wide range of other species, supporting at least half of the Earth's known terrestrial plants and animals. Trees are not only found in forests and other natural ecosystems, but also in urban environments. Most of the human population is concentrated in cities, towns and villages, so urban trees are critical to meet on‐going and future social, economic and environmental challenges. However, many urban tree populations are strongly challenged by a changing climate, outbreaks of pests and pathogens and an urban development with increasingly dense cities and a high proportion of impermeable surface materials. The importance of intraspecific variation needs to be better acknowledged in this context, since poor matching of trees and the local climate and growing conditions can lead to extensive loss of valuable trees. By using the right genetic plant material for the challenging urban environments, a more resilient tree population with a greater diversity and higher capacity for delivering ecosystem services can be gained. Here, we wish to discuss the need to consider intraspecific variation when planning resilient tree populations for urban environments and how seed banks and botanical garden play important roles in efforts to improve the matching of genetic plant material for future environmental challenges. Strategies to enrich urban tree diversity and increase resilience are outlined.

Abstract Background Winter color changes in boxwood occur when foliage shifts from green to shades of yellow, orange, or red. The degree of winter color change in boxwood may influence the aesthetic value of ornamental plants and raise concerns for plant health care professionals. Methods In 2021 and 2022, 40 cultivars of boxwood, including the speciesBuxus sempervirens, B. microphylla, B. sinicavar.insularis, B. harlandii, B. balearica, and hybrids were evaluated for winter color change. In late summer of 2021, 29 plants representing 8 cultivars with intense color change were covered with 75% shade cloth tents or left in full sun from August 2021 through March 2022. In a separate experiment in fall 2021,B. sempervirens‘American’,B. sempervirens‘Rotundifolia’, andB. microphylla‘Winter Gem’ were fertilized or not to determine if slow-release fertilizer could lessen the winter color change. The study was conducted on the transitional area between USDA Hardiness Zones 7b and 8a. Results In 2021,B. sempervirens(61.0%) and hybrid (65.8%) cultivars had the highest percentage of visible color change, followed byB. microphylla(26.1%) andB. sinicavar.insularis(19.1%).Buxus harlandiiandB. balearicahad negligible color change. Shade significantly (P< 0.001) reduced the winter color change observed in March 2022. Fertilization did not influence the visible color change (P= 0.2401). Conclusions The differences in winter color change across boxwood species, cultivars, and under shaded and fertilized conditions will help horticulture practitioners better select cultivars and sites in the landscape relative to winter color change.

Abstract What can the municipal state do to radically transform urban economies? Local government in countries such as the UK has been historically tasked with delivering public services; rarely extending its remit to economic development, let alone community‐led forms promoting economic democracy, at least not since 1980s municipal socialism. But times are changing, and the UK case provides some telling examples of what is now possible. Community wealth building, cooperative development and foundational economy approaches are fast gaining traction in municipalities across the UK, through emergent public‐common‐philanthropic partnerships with progressive think tanks, cooperative development agencies, community enterprises, foundations and charitable trusts. This article explores complexifying relations between the state, the cooperative movement and the non‐profit industrial complex in the pursuit of sustainable and generative economic development as playing out differently in urban policy experiments in the English cities of Preston and Plymouth. The article contributes to current debates on local state restructuring, municipal statecraft and urban entrepreneurialism by critically comparing these place‐based variegations of municipal‐cooperative development with previous waves, and with more financialised and extractivist forms of statecraft, drawing out implications for praxis.

The bioresorbable polymer poly(lactide-co-glycolic acid) (PLGA) coated β-tricalcium phosphate (β-TCP) (β-TCP/PLGA) bone substitute hardens into a stable and porous hard tissue scaffold when exposed to body fluids. Effectiveness of the novel alloplast has been examined in edentulous ridge preservation (ERP) following tooth extraction with subsequent early endosseous dental implant placement; however, it is not clear that the biomaterial is capable of maintaining the edentulous ridge volume to allow for late implant placement due to the rapid bioresorption property of β-TCP. The purpose of this case series is to determine if the β-TCP/PLGA bone substitute is a desirable material for ERP followed by late implant placement. Two patients were treated with ERP and prepared for future endosseous dental implant placement. The β-TCP/PLGA alloplast was placed in the extraction socket. The grafted site of one of the patients was covered by a bioresorbable membrane due to the critical loss of buccal plate. Endosseous dental implant fixtures were placed in both patients 10 months following ERP. Bone core specimens were taken at the time of endosseous dental implant fixture placement and were prepared for histologic assessment. The edentulous ridge volume was successfully maintained for dental implant placement in both cases. Histomorphometric analysis showed that the β-TCP/PLGA alloplast was replaced by newly formed bone with or without guided bone regeneration membrane placement, 54.1% and 34.6%, respectively. The β-TCP/PLGA bone substitute exhibited effectiveness in edentulous ridge preservation followed by late endosseous dental implant placement.

The conversion to HIV-1 single-stranded RNA into double-stranded DNA for nuclear integration is an essential viral step in replication: this process is mediated by Reverse-Transcriptase (RT) and by central polypurine tract (cPPT), a domain where the plus-strand synthesis requires viral primers produced by RNase-H cleavage. Recent studies highlighted the need of investigating the role of RNase-H in RT nucleoside-inhibitors-resistance, because specific mutation(s) could affect cPPT removal and RNase-H cleavage specificity. Thus, the variability of RNase-H and cPPT were studied. HIV-1 subtype-B sequences from 746 drug-naïve and 806 antiretroviral-(ARV)-treated patients were used and analysed. In drug-naïve patients, among 54 RNase-H variable residues, 25 were mutated in >5% of patients, and 7 of them were highly variable (>25%), whilst in ARV-treated individuals, 53 RNase-H variable residues were observed, which 24 were mutated in >5% of patients and 6 of them were highly variable (>25%). Differently, a high conservation was observed in cPPT-area, with no statistically significant differences observed between the two datasets analysed. Nevertheless, in ARV-treated patients the variability of cPPT nucleotide at position 6 was found three times higher with respect to the drug-naïve dataset. The topology of the dendrogram has revealed the existence of a cluster (boostrap=0.98) grouping the A6GcPPT with V531I and S519N RNase-H signatures. These signatures observed within cPPT and mostly in RNase-H, warrant advanced structural analysis to delineate their potential roles in the affinity/recognition of RT and the cleavage capacity of RNase-H. Exploring further the implications such changes may have on drug-resistance may be relevant.

The purpose of this in vitro investigation was to measure the forces generated during the continuous seating and unseating of prefabricated attachment systems used to retain implant overdentures. An experimental design consisting of interchangeable fixture mounts, a radially indexable fixture holder, and a materials testing systems (MTS) machine was used to measure forces generated during the insertion and removal of spherical stud attachments (Straumann, Inc, Waltham, WA). Three separate experiments were conducted measuring the seating and unseating forces of a vertically aligned patrix/matrix assembly, a 20 degrees angled patrix opposing a vertically positioned matrix, and a vertically positioned patrix opposing a 20 degrees angled matrix. For each patrix/matrix combination, three specimens were tested. Measurements were continuously recorded under reproducible conditions in the presence of artificial saliva. All specimens were subjected to 10,000 seating/unseating cycles. Statistical analysis was performed with rank analysis of variance (ANOVA) for a group comparison (alpha= 0.05). Results showed variability in the initial insertion and removal forces among experimental groups and among specimens within each experiment. A marked increase in the seating and unseating forces was recorded for all specimens during the first 300 cycles, followed by a gradual decrease in these forces. The exact p-values for the Kruskal-Wallis test showed no significant difference between the initial and final seating/unseating forces (p > 0.1) nor in the maximum seating/unseating forces (p > 0.6) among the three experimental groups. Spherical stud attachments exhibited consistent seating and unseating forces over 10,000 cycles. A 20 degrees angle between the patrix and matrix had no effect on the overall seating and unseating force values.

We study the nature of cluster selection in Sunyaev-Zel'dovich (SZ) surveys, focusing on single frequency obser- vations and using Monte Carlo simulations incorporating instrumental effects, primary cosmic microwave background (CMB) anisotropies and extragalactic point sources. Clusters are extracted from simulated maps with an optimal, multi-scale matched filter. We introduce a general definition for the survey selection function that provides a useful link between an observational catalog and theoretical predictions. The selection function defined over the observed quantities of flux and angular size is in- dependent of cluster physics and cosmology, and thus provides a useful characterization of a survey. Selection expressed in terms of cluster mass and redshift, on the other hand, depends on both cosmology and cluster physics. We demonstrate that SZ catalogs are not simply flux limited, and illustrate how incorrect modeling of the selection function leads to biased estimates of cosmological parameters. The fact that SZ catalogs are not flux limited complicates survey calibration by requiring more detailed information on the relation between cluster observables and cluster mass.