Bielefeld University

Absence of the endosomal SNAREs vti1a and vti1b results in perinatal death and severe neuronal phenotypes in mice, while lack of one of these proteins results in minor phenotypes. Proteomic differences were investigated to obtain a deeper insight into processes in which vti1a and vti1b are involved. Here we applied a bottom-up shotgun proteomic approach to investigate the differences in wild-type, double heterozygous (DHET), vti1a-/- vti1b-/- double knockout (DKO), vti1a-/- knockout and vti1b-/- knockout cerebral cortices. Single deletions did not affect protein levels significantly. A total of 1725 proteins were detected of which 69 were less abundant and 191 proteins were more abundant in DKO cortices. Many less abundant proteins belonged to cellular components and reactome pathways synapse, synaptic vesicle cycle, vesicle mediated transport, L1CAM interaction, and cholesterol biosynthesis in pathway enrichment analysis. More abundant proteins were enriched in cellular components and Kyoto Encyclopaedia of Genes and Genomes (KEGG)-pathways such as spliceosome, ribosome, carbon metabolism, and ribonucleoprotein complex. Immunoblotting validated reduced expression levels of the tested synaptic vesicle proteins as well as increased amounts of lysophosphatidylcholine acyltransferase 1 (Lpcat1) and neuron-specific gene 2 (Nsg2), which is involved in postsynaptic AMPA-receptor recycling. These data indicate that the synapse and cell adhesion were strongly affected in DKO brains. STATEMENT OF SIGNIFICANCE OF THE STUDY: Distinct populations of neurons and glia cells are generated and organize into layers during brain development. Neurons develop an elaborate morphology to transmit information via axons and synapses to dendrites in receiving neurons. These neurites form via several specialized pathways of vesicle secretion and endocytosis. Fusion between these membranes requires members of the SNARE protein family. Double knockouts of the endosomal SNAREs vti1a and vti1b (DKO) result in perinatal lethality in mice with massive defects in the brain. In this study we compared the proteome of DKO brain cortices with double heterozygous controls to obtain insights into the molecular alterations and affected pathways. DKO brains contained lower amounts of synaptic proteins and proteins involved in cell adhesion, membrane trafficking and cholesterol biosynthesis. Several proteins of spliceosomes, ribosomes and carbon metabolism were more abundant in DKO brains, which may be a consequence of the reduced amounts of synaptic proteins or a shift in cell populations. Lysophosphatidylcholine acyltransferase 1 (Lpcat1) and neuron-specific gene 2 (Nsg2), which is involved in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) recycling, were confirmed to be more abundant by Western blotting. These data point to defects in trafficking especially in the synapse and in cell adhesion, which is required for neurite outgrowth.

The Dementia Leaders programme aims to enhance active public involvement in dementia research by developing a programme for potential coresearchers that includes the perspectives of individuals who experience the disease directly, such as people living with dementia, their informal caregivers and healthcare professionals. The planned study will evaluate this programme (which includes workshops, webinars and online meetings) with a focus on, for example, the content and structure of the programme as well as the experience of the participants regarding their active involvement. A mixed-methods design will be used to evaluate the Dementia Leaders programme. For this purpose, we will conduct semistructured interviews with the participants in the programme. Additionally, we will conduct an online survey after each webinar associated with the programme. The data will be analysed using qualitative content analysis (for the interview data and the open-ended questions included in the online survey) and by employing descriptive statistical methods (for the closed-ended questions of the online survey). Finally, the results obtained via both analytical methods will be synthesised, interpreted and discussed. This study received approval from the ethics committee of the German Society of Nursing Science (Number 25-014). The results will be presented and published alongside the coresearchers at (inter)national conferences and in journals targeting practitioners, caregivers and people living with dementia as well as in peer-reviewed journals.

ABSTRACT Numerous writings on Nazi pedagogy were published by Nazi opponents and refugees as well as by American and English researchers during the Nazi era. Although well-known, these were not included in the German educational discourse on the role of pedagogy and the responsibility of the profession in the Nazi system after 1945. These publications were already well aware of the dual character of Nazi pedagogy, ideological influence, and antisemitic and racist persecution, and mostly follow an ideology-critical approach – thus providing a far-sighted insight into the connection between crime, ideology, and pedagogy. The literature review challenges the post-war German educational research discourse’s neglect of the role of education in fascist societies and aims to reintegrate the forgotten knowledge of opponents, refugees and researchers about Nazi pedagogy into the educational discourse.

In times of species decline and biodiversity loss, large-scale, multi-species surveys are essential for effective conservation planning. Camera traps have become indispensable for wildlife monitoring, providing detailed insights into species abundance

This work-in-progress paper addresses the transition gap between visual, text-based, and complex programming skills in early computer science education. We present a multi-level workshop concept designed to provide a coherent and motivating learning progression across different programming stages. The workshops integrate narrative-driven gameplay with progressively challenging programming tasks in a custom-developed 2D dungeon crawler framework, aiming to support learners in progressing from block-based coding to text-based and open code manipulation. The concept was implemented in collaboration with regional schools and industry partners and evaluated in six workshops with 92 participants. Initial results from pre- and post-surveys indicate high acceptance and a positive trend in programming interest, particularly among female students.

Purpose The aim of this research was to synthesize the literature on workplace outcomes of gender diversity in sport leadership using a realist synthesis approach, helping to reduce stakeholders’ uncertainty regarding potential consequences of increased gender diversity. Design/methodology/approach The articles were identified using rapid evidence assessment (n = 24) and synthesized in terms of adopted theories and empirical evidence. Findings The theories can be classified into individual-level theories explaining how women differ from men in terms of characteristics and behavior, and group-level theories explaining potential consequences of group diversity, not specifically gender diversity. The identified workplace outcomes include structural characteristics, internal functioning, organizational orientation and performance (incl. financial performance), with gender diversity mainly having a positive or no association. The complexity of sport in terms of types of sport organizations (for-profit, non-profit, public) and workers (volunteers, paid staff) was not considered theory-wise; only some outcomes were sport-specific. Research limitations/implications Since studies mostly applied psychological theories from the corporate literature to sport, future research may consider the peculiarities of the sport sector (e.g. non-profit organizations, volunteers) to a greater extent. Greater consideration could also be given to the collection of longitudinal data that allow identifying causes and effects as well as changes over time and to studying gender diversity in sport leadership from an intersectional lens. Originality/value This article is the first to integrate sport literature related to outcomes (as opposed to antecedents) of gender diversity in sport leadership.

Consider the following two popular and plausible principles. First principle: facts about what you ought to do are tightly bound up with facts about what there’s reason to do. For example, if you ought to Φ, then there must be more reason for you to Φ than there is for you to not-Φ. Second principle: ought is ‘upwards monotonic’—if Φing entails Ѱing, then if S ought to Φ is true, so is S ought to Ѱ. I argue that these two principles are incompatible. If I’m right, then respecting the connections between reasons and oughts requires giving up monotonicity, and so requires giving up the classical semantics for deontic modals. We need something new. I sketch a non-monotonic meaning for ‘ought’ which builds the ought/reason connections directly into the semantics, while aiming to accommodate the data that made monotonicity look attractive in the first place.

Zusammenfassung Der Beitrag untersucht die Weitergabe und den Wandel familial fundierter Werte und religiöser Überzeugungen unter Bedingungen der Pluralisierung und Säkularisierung. Im Zentrum stehen junge Frauen aus freikirchlichen russlanddeutschen Familien, deren biographische Selbst- und Lebensentwürfe anhand narrativer Interviews rekonstruiert werden. Die theoretische Rahmung dafür bilden werttheoretische Perspektiven, die Werte als identitätsstiftende und handlungsorientierende Vorstellungen eines guten, gelingenden Lebens begreifen. Die Analyse zeigt übergreifend eine hohe Kontinuität des Familialen, wobei zwei grundsätzliche Muster der Wertetransmission unterschieden werden können. Entscheidend für diese Muster scheinen dabei weniger die konkreten Werte als die Form ihrer familialen Vermittlung. Der religiöse Glaube wird in den meisten Fällen weitergeführt und funktional ausgerichtet. Er dient einerseits als Bewältigungsstrategie gegenüber gesellschaftlichen Anforderungen einer eigenverantwortlichen Lebensgestaltung, wird andererseits aber auch von diesen Anforderungen durchdrungen. Die Untersuchung leistet damit einen Beitrag zur empirischen Erforschung der lebensgeschichtlichen Entwicklung von Werten während der Adoleszenz und in den Kontexten von Familie, Migration und Religion.