Bosque School

Agave angustifolia es una especie que se utiliza como materia prima para producción de mezcal en el sur del Estado de México, por lo cual se ha incrementado la demanda de planta para el establecimiento de plantaciones. El cultivo de tejidos vegetales ofrece una alternativa para la propagación masiva de plantas; por ello, el objetivo de la presente investigación fue desarrollar un protocolo para la propagación in vitro de la especie. Bajo condiciones in vitro se realizó la germinación, multiplicación y elongación de brotes, y formación de raíces, mientras que en vivero se hizo la aclimatación de plantas. El porcentaje de germinación fue superior a 80 % y se registraron diferencias entre los tratamientos de desinfección y medios de cultivo. El uso del medio MS al 100 %+0.003 mg L-1 de BAP+0.002 mg L-1 de AIB+0.1 mg L-1 de pantetonato de calcio produjo mayor número de brotes por planta a los 90 y 120 días. Con el medio de cultivo MS al 75 % se obtuvo 100 % de enraizamiento, con un promedio 6.5 raíces por planta y 45 mm de longitud de la raíz. En la etapa de aclimatación en vivero se presentó 100 % de sobrevivencia en los tres sustratos evaluados y se observaron diferencias en altura, diámetro y materia seca. En conclusión, se generó un protocolo completo para la micropropagación de Agave angustifolia, desde la germinación in vitro hasta su aclimatación en vivero.

The complex structure of the liver and its elaborate hemodynamics can cause hepatic pseudolesions on contrast-enhanced imaging, making the interpretation of diagnostic liver imaging challenging. Aberrant gastric veins are rare; most of their epidemiology data comes from small single-center studies. While current literature suggests that pseudolesions originating from aberrant gastric veins mainly present as hyperdense defects, some cases can also show up as hypodense, as shown in these cases. Differences in flow rates between the portal and aberrant veins and the timing of the scans could explain this contradiction. Identifying aberrant gastric veins on cross-sectional imaging is crucial because they could be misdiagnosed as liver lesions, granting further unnecessary workups or invasive procedures. Aberrant gastric veins can also act as pathways for the spread of gastric cancer. This manuscript presents one aberrant right gastric vein and two aberrant left gastric veins causing hepatic pseudolesions.

Abstract Purpose: To evaluate 12-month surgical outcome of Kahook Dual Blade (KDB) goniotomy in combination with cataract surgery in Latino patients with open angle glaucoma (OAG) and ocular hypertension (OHT). Methods: This retrospective study included 45 eyes of 40 patients who underwent KDB goniotomy combined with cataract extraction from January 2016 to September 2020 at two centers in South America. Primary outcome was surgical success was defined as ≥20% intraocular pressure (IOP) reduction or ≥1 medication reduction from preoperative without additional IOP-lowering procedures and an IOP ≥5 mmHg or ≤21 mmHg. Additionally, we used 2 cutoffs values for success of IOP ≤18 and ≤15 mmHg. Secondary outcomes included: IOP, medication use, best corrected visual acuity, complications and failure-associated factors. Results: Success rates at 12 months with cutoff limits of 21, 18 and 15 mmHg were 84.3%, 75.6% and 58.7%, respectively. At 12 months, mean preoperative IOP significantly decreased from 19.23 ± 0.65 mmHg on 2.33 ± 1.04 medications to 14.33 ± 0.66 mmHg on 0.59 ± 0.94 medications, with 62% of eyes free of hypotensive medication. Eyes that developed postoperative IOP spikes had higher risk for failure using the cutoff limit of IOP ≤18 mmHg with a hazard ratio of 3.6 (95% confidence interval [CI], 1.80-7.13; p<0.001). There were no serious ocular adverse events. Conclusions: KDB combined with cataract extraction showed safety and efficacy for decreasing IOP and the number of hypotensive medications in Latino patients after one year follow-up. Intraocular pressure spikes were associated with a higher risk for failure.

Microbial diversity of cave pools, especially vadose pools, has received relatively little attention. To help fill this gap, this study reports on the bacterial diversity of 17 pools in three New Mexican arid land caves: Carlsbad Cavern, Lechuguilla Cave, and Hell Below Cave. These pools are spread throughout the caves and, with two exceptions, are not connected. The pools share a basic water chemistry, with fresh water of the calcium-magnesium-bicarbonate type. These 17 pools have Chao1 values between 40 and 1738; the Shannon diversity averages 4.6 ± 1.1, ranging from 2.6 to 6.4; and the Simpson averages 0.881 ± 0.099, ranging from 0.622 to 0.981. No two pools had the same communities, even at the phylum level. Nitrospirae, Alphaproteobacteria, Betaproteobacteria and Gammaproteobacteria were found >5% abundance in nine or more cave pools. Actinobacteria, Chloroflexi, Fibrobacteres, Firmicutes and Plantomycetes were at >5 % in four to six pools. Of the top ten widespread bacterial genera, Nitrospira was found in all pools, with >5 % in eleven pools. Other common genera include Polyclorovans, Propionibacterium, Polaromonas, Haliangium, Bacillus, Subgroup 6 uncultured Acidobacteria, Candidatus Omnitrophica, and uncultured Nitrosomonadaceae. Presence of several potential nitrogen cycling bacteria (e.g., Nitrospira) in the study pools suggests that nitrogen cycling may be an important bacterial role. There is some evidence of human contamination, particularly in the heavily visited Big Room, Carlsbad Cavern, but it is not the dominant control. Rather than a single stable cave pool community, adapted to the cave pool ecosystem, the data show 17 different communities, despite relatively similar conditions. The data support the hypothesis that each pool is a unique, isolated ecosystem, with differences likely caused more by the isolation of each pool than by variable chemistry. Thus, the common habit of grouping samples, while useful for some questions, may not capture the diversity present in cave ecosystems.

Introduction:The objective of this study was to compare the mechanical and structural properties of the nickel-titanium (Ni-Ti) alloy already used in endodontics with titanium-molybdenum (Ti-Mo) and titanium-niobium (Ti-Nb) alloys to determine if these can be suggested in the manufacture of endodontic files. Methods and Materials: Orthodontic wires made of the different alloys were used. The previously mentioned alloys were characterized by energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD) and torsion tests. Cyclic fatigue tests were performed on a simulated canal with a curvature of 86° to 375 rpm. The fractured surfaces of the wires were observed by means of scanning electron microscopy (SEM). A Kruskal-Wallis test and U Mann Whitney test were used to determine significant differences in cyclic fatigue between groups. Results: In the mechanical tests, similar values of torsion were found for the three alloys. In XRD, the Ti-Nb showed less structural changes. In the cyclic fatigue test, Ti-Nb was found to be significantly more resistant with respect to Ni-Ti and Ti-Mo. Conclusion:Based on our in vitro study, Ti-Nb is suggested as a possible alloy for the manufacture of rotary files due to its impressive properties.

We tested how sediment trapping by hydroelectric dams affects tropical estuaries by comparing two dammed and two undammed rivers on Mexico's Pacific coast. We found that dams demonstrably affected the stability and productivity of the estuaries. The two rivers dammed for hydroelectricity had a rapid coastal recession (between 7.9 and 21.5 ha year-1) in what should otherwise be an accretional coastline. The economic consequences of this dam-induced coastal erosion include loss of habitat for fisheries, loss of coastal protection, release of carbon sequestered in coastal sediments, loss of biodiversity, and the decline of estuarine livelihoods. We estimate that the cost of the environmental damages a dam can cause in the lower part of basin almost doubles the purported benefits of emission reductions from hydroelectric generation.

This paper assess initial collateral effects of the use of hyperthermia in breast cancer treatment by means of an electromagnetic simulation. The breast model was radiated by microwaves with a waveguide at 2.45 GHz and the power density distribution inside the breast was calculated. A comparison between the power density in the tumor and other breast tissues (skin, fat, and muscle) is presented. Results show that the location of the microwave applicator is a factor that determines the unwanted overheating of tissues close the tumor.

Colloidal mixtures represent a versatile model system to study transport in complex environments. They allow for a systematic variation of the control parameters, namely size ratio, total volume fraction and composition. We study the effects of these parameters on the dynamics of dense suspensions using molecular dynamics simulations and differential dynamic microscopy experiments. We investigate the motion of small particles through the matrix of large particles as well as the motion of large particles. A particular focus is on the coupling of the collective dynamics of small and large particles and on the different mechanisms leading to this coupling. For large size ratios, of about 1 : 5, and an increasing fraction of small particles, the dynamics of the two species become increasingly coupled and reflect the structure of the large particles. This is attributed to the dominant effect of the large particles on the motion of the small particles, which is mediated by the increasing crowding of the small particles. Furthermore, for moderate size ratios of about 1 : 3 and sufficiently high fractions of small particles, mixed cages are formed and hence the dynamics are also strongly coupled. Again, the coupling becomes weaker as the fraction of small particles is decreased. In this case, however, the collective intermediate scattering function of the small particles shows a logarithmic decay corresponding to a broad range of relaxation times.