East Georgia State College

The screening analysis of loss-of-function alleles in Arabidopsis thaliana revealed a mutation in the At3G20550 gene, called DAWDLE (DDL). The mutation in the DDL gene causes pleiotropic phenotypes and reduced the levels of several microRNAs. The DAWDLE gene encodes a protein with a Fork Head-Associated (FHA) domain, found in large range of proteins with significant cellular processes in prokaryotes and eukaryotes. However, it is not completely known whether the FHA domain and C-terminal region of the DDL are necessary for its function. The aim of this study was to determine the function of both regions by conducting a phenotypic analysis of point mutations spanning the FHA domain and C-terminal region in DDL Targeted Induced Local Lesions IN Genome (Tilling) screen was performed in the Columbia erecta-105 background of Arabidopsis resulting in point mutations spanning both regions of DDL. The mutants were phenotypically characterized. Height of the plant, hypocotyl and root length, and fertility were measured. Phenotypic analyses of the mutants revealed ddl phenotypes of varying degrees in different organs. Reduction in fertility and shortening in root, hypocotyl and stem lengths of the Tiller mutant lines suggest that the FHA domain and C-terminal region may require for DDL function in Arabidopsis. Key words: Dawdle, Fork Head-Associated Domain, Targeted Induced Local Lesions in Genome, Ethyl Methane Sulfonate, Arabidopsis

This work is aimed at modelling the progressions of COVID-19 cases in time in relation to meteorological determinants, in large cities of Brazil, Italy, Spain, and USA, and predicting the viability of SARS-CoV-2 virus in different weather conditions based on models. Our statistical analysis indicates that the spreading of infection does not vary exponentially with time and hence, does not have similarity with law of mass action in chemistry as considered in general for spreading of infectious diseases. New models are constructed showing the relationship of the I' (the number of infected individuals divided by the total population of a city) with the independent variables-time, temperature, relative humidity, and wind velocity. The regression models fitting in the data are statistically validated by : 1) plot of observed and predicted response; 2) standardized residual plots showing the characteristics of errors; 3) adjusted value; 4) the p value for the parameters associated with the various independent variables; and 5) the predictive power of the model beyond data points. Models indicate that 1) the transmission of COVID-19 could be relatively high either for elevated temperatures with lower relative humidity or for lower temperatures with higher relative humidity conditions; 2) disease transmission is expected to be reduced more with higher wind velocity; 3) for meteorological factors remaining same, the rate of increase in the number of COVID-19 cases increases in one model with a constant rate and in the other two with varying rates in time. These transmission features seem to have connections with the structural components of the SARSCoV- 2 virus. Under suitable meteorological conditions, the partial natural disappearance of COVID-19, pandemic could be possible. New models for Ij may be considered to understand the viability of the virus in the environment and future transmission of COVID-19.

DAWDLE (DDL) is a conserved forkhead-associated (FHA) domain-containing protein with essential roles in plant development and immunity. It acts in the biogenesis of microRNAs (miRNAs) and endogenous small interfering RNAs (siRNAs), which regulate gene expression at the transcriptional and/or posttranscriptional levels. However, the functional mechanism of DDL and its impact on exogenous siRNAs remain elusive. Here, we report that DDL is required for the biogenesis of siRNAs derived from sense transgenes and inverted-repeat transgenes. Furthermore, we show that a mutation in the FHA domain of DDL disrupts the interaction of DDL with DICER-LIKE1 (DCL1), which is the enzyme that catalyzes miRNA maturation from primary miRNA transcripts (pri-miRNAs), resulting in impaired pri-miRNA processing. Moreover, we demonstrate that DDL interacts with DCL3, which is a DCL1 homolog responsible for siRNA production, and this interaction is crucial for optimal DCL3 activity. These results reveal that the interaction of DDL with DCLs is required for the biogenesis of miRNAs and siRNAs in Arabidopsis (Arabidopsis thaliana).

"A Review of “Patriotism and Public Spirit: Edmund Burke and the Role of the Critic in Mid-18th Century Britain”." Perspectives on Political Science, 43(4), pp. 236–237

Prium non nocere or ‘‘First, do no harm’’ is traditionally associated with the Hippocratic oath that medical doctors take. The premise of this quote is that no matter what end result you are attempting to achieve, whether it be curing a patient or educating a child, your first consideration should be that no harm be done in pursuit of the goal. When this is applied to the special education classroom, mandatory high-stakes testing for students with intellectual disabilities violates this principle on many levels. According to the American Association on Mental Retardation website, the definition of mental retardation, another term for intellectual disabilities, is A disability characterized by significant limitations both in intellectual functioning and in adaptive behavior as expressed in conceptual, social, and practical adaptive skills. This disability originates before 18. [There are five assumptions essential to the application of this definition]: 1. Limitations in present functioning must be considered within the context of community environments typical of the individual’s age, peers, and culture. 2. Valid assessment considers cultural and linguistic diversity as well as differences in communication, sensory, motor, and behavioral factors. 3. Within an individual, limitations often coexist with strengths.

Journal Article Laughlin McDonald. A Voting Rights Odyssey: Black Enfranchisement in Georgia. New York: Cambridge University Press. 2003. Pp. viii, 254. Cloth $55.00, paper $20.00 Get access McDonald Laughlin. A Voting Rights Odyssey: Black Enfranchisement in Georgia. New York: Cambridge University Press. 2003. Pp. viii, 254. Cloth $55.00, paper $20.00. Thomas Adams Upchurch Thomas Adams Upchurch East Georgia College Search for other works by this author on: Oxford Academic Google Scholar The American Historical Review, Volume 110, Issue 1, February 2005, Pages 182–183, https://doi.org/10.1086/ahr/110.1.182 Published: 01 February 2005