Endangered Wildlife Trust

Illegal trade in leopard tortoises Stigmochelys pardalis is widespread, yet the origins of confiscated S. pardalis are often unknown. Accurate assignment to source populations is essential for informed conservation management and release. We expanded and tested a genetic reference database for assigning S. pardalis to their original wild populations in KwaZulu-Natal Province, South Africa, and Eswatini. We added 50 newly genotyped wild S. pardalis sampled across these regions to the database and then evaluated the updated database by sampling and assessing 50 S. pardalis of unknown geographic origin held at a rehabilitation centre in KwaZulu-Natal. Genetic assignment indicated that only a single S. pardalis at the rehabilitation-centre may have originated from KwaZulu-Natal, highlighting the extent of illegal trade, translocation, and possession of S. pardalis. Individuals of verified regional origin were subsequently rehabilitated and translocated for soft release at appropriate sites.

Giant pandas (Ailuropoda melanoleuca), a flagship endangered species under priority preservation in China, remain poorly understood in terms of their testicular physiology and the mechanisms underlying spermatogenesis. Testicular peritubular cells (TPTCs), a crucial somatic cell type surrounding seminiferous tubules, secrete growth factors such as GDNF and CSF1 and release inflammatory factors such as IL-6 and IL-1β, contributing to the testicular niche and immune homeostasis. The contraction of TPTCs also facilitates the transport of sperm towards the epididymis. Nonetheless, TPTCs tend to undergo replicative senescence in vitro, which is a hinderance to their in-depth study. Here, we generated an immortalized monoclonal cell line with TPTC identities from giant pandas via lentiviral transduction of SV40 large T antigen into the cells and the subsequent clonal isolation through limiting dilution. The generated cell line, designated PD-TPTCs, demonstrated unlimited proliferative capacity and has been cultured for over five months and passaged more than 50 times to date. Characterization of PD-TPTCs revealed stable expression of key TPTC markers including ACTA2, MYH11, CNN1, and AR. Moreover, PD-TPTCs could respond to ATP and forskolin (FSK) stimulation with a pro-inflammatory gene expression profile and increased steroidogenic activity, respectively, and they were also amenable to lipofection. As such, the generated PD-TPTC line represents a promising cellular model for future mechanistic studies on the testicular niche, spermatogenesis, and reproductive disorders in giant pandas, laying the foundation for the development of novel assisted reproductive technology (ART) in this endangered species.

Wetlands are relatively common and highly important biodiversity sites in southern Africa despite their prevailing dryland environment. Although there is very little known about its wetland sites, Angola is no exception to this regional picture and 11 sites have been recently proposed as Ramsar sites since Angola joined the Ramsar Convention in June 2021. Despite the absence of information on wetlands in the country, here we identify the most likely types of wetlands to be found in Angola, and their likely favored locations, based on global environmental datasets (climate, topography, hydrology, and ecosystems). Based on this regional analysis, we then identify the most likely ways in which local communities may make use of these different wetlands, following patterns of wetland use found elsewhere in southern Africa. This analysis shows that wetlands are relatively common in Angola as mountain peatlands, riparian and river valley wetlands, and coastal estuaries/lagoons, although they have hitherto been significantly underreported in the literature. A better appreciation of the role of wetlands in ecosystem service provision in Angola may lead to concerted efforts to map and characterize them and then protect them under relevant legislation.

ABSTRACT Despite their ecological significance, frogs receive far less public support and funding for research and conservation than that afforded to more charismatic animals. An emerging perspective suggests that conservation success is dependent on understanding the cognitive, emotional, and behavioural aspects that impact attitudes and behaviours towards biodiversity. This mixed-methods study attempted to shed light on how three communities in the vicinity of Nature’s Valley in the Western Cape province of South Africa, perceive, interact with, and consider the protection of frogs. Overall, the three communities slightly disliked frogs, however, geo-specific variability between demographic profiles, cultural beliefs and myths, and memorial background evidently strongly impact human-frog interaction and willingness to tolerate and protect them. This complex interplay between cultural and personal factors, as well as grouped bio-spherical values, led to the conclusion that to ensure the success of frog conservation efforts, a generalist approach to pro-frog behavioural change should be followed by focusing on pro-environmental behavior in general first rather than attempting to change a specific aspect of human-frog interaction from the outset.

Facultative scavenging can be observed across a large range of carnivorous mammals, but is an uncommon behavioural trait in cheetahs (Acinonyx jubatus). Very few incidents of cheetahs scavenging have been reported, with no explanation given as to why it may occur. In this paper we provide three more observations of cheetahs scavenging between 2019 and 2023 in three different protected areas in South Africa and Malawi. We suggest a common factor between these observations, namely that all adult individuals involved were temporarily housed in holding enclosures (bomas) during relocation processes and were provisioned with carrion as supplemental feed. The observed scavenging events could also have been influenced by the easy access of food in a situation where food acquisition was potentially difficult (i.e. old age, loss of hunting partner, mother with cubs). We hypothesise that these contributing factors, combined with the prior exposure of the adult individuals to eating carrion, could be a potential explanation for our observations of cheetahs scavenging. These observations provide a basis for further research into cheetah scavenging behaviour and the potential impacts of translocations that require prolonged holding periods on wildlife behaviour post-release. Understanding these behavioural shifts is crucial for cheetah conservation, as successful reintroduction efforts depend on the ability of cheetahs to adapt to new environments and food acquisition strategies.

The African Penguin Spheniscus demersus has been considered a threatened species since 1984 and, aside from a short period around 2000, its population has been in constant decline since at least the 1950s. By combining counts of the numbers of breeding pairs made at 26 colonies in South Africa and Namibia between 1979 and 2023 with Bayesian state–space models we reassess the species’ conservation status. The breeding population has declined by 77.8% (95% credible intervals: 71.8–84.6%) over the last 30 years (3 generations), from ∼44 300 breeding pairs in 1993 to ∼9 900 pairs in 2023. This falls just below the threshold for a global IUCN Red List status of Critically Endangered (CR) under criterion A2. However, the decline in Namibia exceeds that threshold at the national level (30-year decline = 82.4%, 78.2–86.2%) following the loss of ∼3 600 breeding pairs there in the last 5 years. In South Africa, the Western Cape population is now declining at <1.5% per annum, but the annual rate of decline in the Eastern Cape has worsened substantially, reaching 13% over the last 10 years. Overall, the global population has more than halved in the last decade and has fallen below 10 000 pairs for the first time. Moreover, “moving window reductions” over 3 generations using observed and projected population trajectories (up to the year 2033) indicate that the decline of the African Penguin population will exceed the 80% CR threshold (under criterion A4ab) with a high probability by 2028. Accordingly, we propose that the African Penguin should be transferred to the IUCN Red List category Critically Endangered.

In an effort to halt the global decline of large carnivores, reintroductions have become increasingly popular to establish satellite populations and reduce the risk of stochastic events. These artificial range expansions are typically formed by a small number of founders, which can lead to changes in population genetic structure. For instance, serial founder events can lead to neutral and even deleterious alleles reaching higher than expected frequencies along the front end of an expansion, referred to as gene surfing. One of the world’s most extensive range expansion programmes has been for endangered African wild dogs (Lycaon pictus). In this study, we examine the effect of continent-wide translocations on spatial genetic diversity, by determining what effect genetic surfing has on population structure in wild dogs, and measuring how long it will take for population structure to homogenize in the face of ongoing dispersal. We used a set of microsatellite loci to look at surfing alleles in five populations across southern Africa, and simulated the movement of these alleles forward in time under the current demographic scenario. We found that it would take about 150 generations for the expanding population to be 50% introgressed with genes from the free-roaming population. With the current rate of translocations, genetic differentiation in southern Africa will disappear, overturning the effects of genetic drift or surfing alleles. Understanding genetic patterns in expanding populations is of great interest to conservation, and we demonstrate that reintroduction programmes can help restore genetic diversity, and consequently adaptive potential, in recovering wildlife populations.

The south-western Indian Ocean (SWIO) is a region of global importance for marine mammal biodiversity, but our understanding of most of the species and populations found there is still rudimentary. The Indian Ocean Network for Cetacean Research (IndoCet) was formed in 2014 and is dedicated to the research of all cetacean species across the SWIO. Since 2019, there have been efforts to create a regional network for coordinated response to stranding events as well as training and capacity building in the SWIO region. The present analysis represents a first investigation of stranding data collected by various members and collaborators within the IndoCet network, covering over 14,800km of coastline belonging to nine countries/territories. Between 2000-2020, there were 397 stranding events, representing 1,232 individual animals, 17 genera and 27 species, belonging to six families: four balaenopterids, one balaenid, one physeterid, two kogiids, six ziphiids and 14 delphinids. Seven mass strandings were recorded: two were composed of three to 20 individuals and five composed of &gt;20 individuals. Spatial analysis of stranding events indicated that local spatio-temporal clusters (excessive number of events in time and geographic space) were present in all countries/territories, except for the Comoros. The only significant cluster was detected on the southwest coast of Mauritius, just west of the village of Souillac. The SWIO region predominantly comprises relatively poor countries/territories, but imminent Blue/Ocean economy developments are prevalent throughout the region. This study highlights the importance of establishing baselines upon which any future potential impact from anthropogenic developments in the region can be measured.

Abstract The proliferation in mesoherbivore numbers and their associated impact on the detrital ground cover were deemed responsible for the decline in fossorial herpetofaunal densities in Ndumo Game Reserve during the early 2000s. Subsequent increased poaching pressure and wildlife declines allowed the reassessment of the sand forest fossorial herpetofaunal community to test this assumption. We found no significant increase in the two most abundant soil‐living reptiles despite significant mesoherbivore declines. This may indicate that either fossorial herpetofaunal numbers increase very slowly, or that the initial two survey methodologies were incomparable. Alternatively, some unknown environmental factor may be suppressing populations or the survey results are not representative of the fossorial herpetofaunal community.

Abstract A dense Ecklonia radiata (Laminariales) kelp forest extending at least 35 km has been found between 45 and 60 m depth range within the mesophotic zone inside the iSimangaliso marine-protected area (MPA) at the iSimangaliso Wetland park World Heritage Site on the east coast of South Africa. This is the first visual confirmation of the occurrence of E. radiata beds in subtropical South Africa, in an area situated between the tropical and subtropical bioregions, in an area that spans the Natal and Delagoa bioregions of the south-western Indian Ocean, more than 350 km north of its previously documented South African range. The kelp was found to be present across the length of the MPA, but dense beds were present only in the southern Natal bioregion, with sparse occurrences observed elsewhere on soft-coral and sponge-dominated reefs in the upper mesophotic zone. The footage was collected in November 2020, May 2021 and November 2022 during remotely operated vehicle and drop camera surveys of the mesophotic zone inside the MPA. This discovery adds to the body of knowledge on the global distribution of Laminariales populations in deep tropical and subtropical settings and the diversity of habitats within South Africa's largest coastal MPA.