Fuling Center Hospital of Chongqing

Diabetic nephropathy (DN) represents a major complication of diabetes mellitus (DM) with limited early diagnostic biomarkers. This study investigated the clinical significance and molecular mechanism of long non-coding RNA (lncRNA) VCAN-AS1 in DN pathogenesis. The patients with diabetes mellitus, including 85 with DN and 66 without nephropathy, were enrolled, along with 62 healthy controls. Serum VCAN-AS1 levels were detected using qRT-PCR. Clinical parameters and renal injury markers were analysed for correlations with VCAN-AS1. Invitro, high glucose-treated human renal tubular cells (HK-2) underwent genetic interventions including VCAN-AS1 knockdown and miR-205-5p inhibition. Functional assessments encompassed cell viability, oxidative stress, fibrosis and inflammation. Dual-luciferase reporter assays validated molecular interactions. Serum VCAN-AS1 expression showed a progressive increase across healthy controls, diabetes mellitus patients and DN patients. It exhibited strong diagnostic efficacy for DN with an AUC of 0.879. Elevated VCAN-AS1 correlated with deteriorated renal function parameters. VCAN-AS1 was identified as an independent risk factor for diabetes mellitus progression to DN. High glucose exposure up-regulated VCAN-AS1 in HK-2 cells, promoting cellular injury phenotypes. Rescue experiments confirmed that miR-205-5p inhibition reversed the protective effects of VCAN-AS1 knockdown. Mechanistically, VCAN-AS1 directly bound miR-205-5p, suppressing its expression and consequently enhancing vascular endothelial growth factor A (VEGFA) expression. VCAN-AS1 serves as a potential diagnostic biomarker for DN and promotes disease pathogenesis through the miR-205-5p/VEGFA axis, highlighting its potential as a therapeutic target.

Abstract In practical engineering applications, single-channel information often suffers from weak directionality and limited representational capability. To address these challenges, this paper proposes a channel-weighted adaptive cross-domain method for fault diagnosis of rolling bearings under different operating conditions. Firstly, through multi-channel feature fusion, multi-channel samples of the same category are concatenated along the channel dimension to construct channel-representational data with multi-angle, multi-dimensional key features, thereby enhancing the sample's representational capability. Secondly, adaptive channel dynamic weighting mechanism is designed, which utilizes a weight generator to dynamically learn the importance coefficients of each channel. By fusing multi-channel features through weighting, the interference of high-difference channels is effectively suppressed, reducing the risk of negative transfer. Finally, to avoid the problem of loss accumulation in traditional multi-source cross-domain methods, a unified subdomain alignment loss is constructed in the manifold space of the multi-channel fused samples. With the help of A-distance, unsupervised inter-domain distribution adaptation is achieved, and the feature distributions of the source and target domains are aligned to overcome domain shift. Experiments on two sets of rolling bearing fault diagnosis tasks under different operating conditions show that the proposed method improves the average accuracy by 8.12% compared to classical multi-source transfer models, with a significant reduction in parameter count. This verifies method's advantages in structural simplicity, difference robustness, and engineering applicability.

Resistance to 5-fluorouracil-based chemotherapies presents a major clinical challenge in colorectal cancer (CRC) treatment, leading to elevated recurrence rates. Recent findings indicate that enhanced metabolic plasticity in cancer cells plays a crucial role in drug resistance development, though the exact mechanisms remain uncertain. In this study, we demonstrate that the lipophagy-triggered fatty acid oxidation (FAO) is involved in maintaining the adenosine triphosphate (ATP) levels upon drug administration. Using mass spectrometric imaging, we identified arachidonic acid (AA) as a critical lipid mediator of intracellular selective autophagy and energy balance in resistant cancer cells. The high level of arachidonate metabolism was associated with poor response to chemotherapy in clinical practice. Mechanistically, accumulated AA induced phosphorylation of PLINs via the LOXs/PKCζ pathway and stimulated the production of free fatty acid (FFA) driven by lipophagy. Importantly, we further certified that inhibition of AA-associated lipophagy specifically sensitize drug-resistant colorectal cancer cells to chemotherapy. This study offers insights into the mechanism of acquired chemoresistance, suggesting that targeting AA-associated lipophagy could be a potential therapeutic strategy to overcome drug resistance in colorectal cancer.

Background: Visceral fat plays a central role in cardiometabolic risk among people with type 2 diabetes mellitus (T2DM), yet its assessment in routine clinical practice remains largely dependent on imaging techniques or indirect anthropometric measures. Identifying accessible blood-based markers that reflect visceral adiposity may facilitate improved phenotyping in this population. This study aimed to investigate whether circulating coiled-coil domain-containing protein 3 (CCDC3) reflects visceral fat accumulation in adults with T2DM. Methods: Public RNA-sequencing datasets and human adipose tissue samples were analyzed to identify CCDC3 as a visceral fat-enriched secretory gene. In this cross-sectional study of 160 adults with T2DM undergoing dual-energy X-ray absorptiometry, plasma CCDC3 was measured by ELISA. Associations between plasma CCDC3 and visceral fat area (VFA) were examined using multivariable regression. Logistic regression models for abdominal obesity (VFA ≥ 100 cm2), with and without CCDC3, were evaluated using receiver operating characteristic (ROC) analysis, calibration curves, decision curve analysis (DCA), and Shapley additive explanations (SHAP). Results: Circulating CCDC3 levels were positively associated with VFA (β = 3.11, p < 0.001), independent of demographic and metabolic factors. Incorporating CCDC3 into the baseline model significantly improved discrimination of abdominal obesity (AUC 0.820 vs. 0.663; p = 0.009). Calibration curves and DCA supported better model fit and higher net clinical benefit with CCDC3. SHAP analysis showed that CCDC3 contributed the greatest incremental importance beyond waist circumference, sex, and age. Conclusions: Circulating CCDC3 may serve as a blood-based biomarker reflecting visceral adiposity in adults with T2DM and provides complementary information beyond traditional anthropometric measures.

ObjectivePostoperative pain is the most notable issue after cesarean section (CS). The contributing factors include hyperalgesia, anxiety, depression, sleep disorders, and inflammation. In this study, we explored the effects of esketamine on pain, hyperalgesia, depression, anxiety, sleep disorders, and inflammation after CS.MethodsThis randomized, blinded, controlled trial enrolled single-term pregnant women scheduled for elective CS. This trial was a single-center study conducted at Chongqing University Fuling Hospital. A simple randomization method was used. SPSS version 26.0 generated random numbers. The participants were randomly included in the esketamine group (group E: intravenous esketamine 0.5 mg/kg + sufentanil 4 μg/kg followed by patient-controlled intravenous analgesia with esketamine 0.5 mg/kg) or the control group (C: normal saline + sufentanil 4 μg/kg PCIA). The primary outcome was the maximum pain numerical rating scale (NRS) score within 24 h postoperatively. The secondary outcomes included pain NRS scores for moving incision, visceral, and rest incision pain at 0–6 h, 6–12 h and 12–24 h; pressure pain threshold and tolerance at 30 min and 24 h postoperatively; PCIA drug consumption, number of compressions, and dosage of rescue analgesics; time to first PCIA compression; serum C-reactive protein (CRP) at 24 h; incidence of drug-related side effects; and rates of anxiety, depression, and sleep disorders on postoperative day 2.ResultsNinety-eight women were randomly included in group E (n = 50) or C (n = 48). Group E showed significantly lower maximum NRS pain scores within 24 h (5 [4–5] vs. 6 [5–6], P < 0.0001) and relieved rest incision, visceral, and moving incision pain at all time points. The PCIA compression was significantly delayed and CRP levels, as well as the incidence of postoperative depression, anxiety, and sleep disorders, were lower in group E. There were no statistically significant differences in hyperalgesia or side effects between the groups.ConclusionIntravenous esketamine could effectively reduce postoperative pain, psychological disorders, and inflammation after CS.This study was registered in the Chinese Clinical Trial Registry with registration number ChiCTR2300078310.

To evaluate the efficacy and safety of different methods of managing the distal hernia sac in preventing seroma after laparoscopic transabdominal preperitoneal repair (TAPP) for large indirect inguinal hernias. A retrospective analysis was conducted on 163 male patients with unilateral large indirect inguinal hernias (hernia sac diameter > 5cm) who underwent TAPP at the Department of Gastrointestinal Surgery, Fuling Hospital of Chongqing University between January 2020 and January 2025. Based on the method of hernia sac management, patients were divided into three groups: Group A (n = 38) underwent complete dissection of the hernia sac; Group B (n = 61) underwent transection and abandonment of the distal sac; Group C (n = 64) underwent midline transection of the lateral hernia sac combined with lateral fixation of the residual stump. Perioperative indicators, postoperative VAS scores, and complication rates were compared among the groups. All 163 patients successfully completed the surgery. Operative time was significantly longer in Group A (94.16 ± 21.93min) compared to Groups B (80.59 ± 27.00min) and C (93.97 ± 23.49min) (P = 0.027), whereas no significant difference was detected between Groups B and C. The time required for hernia sac management also differed significantly across groups (P < 0.001), being longest in Group A, followed by Group C, and shortest in Group B. In terms of hospital stay, Group B (6 [5, 7] days) stayed significantly longer than both Group A and Group C (both 5 [4, 7] days) (P = 0.005). The incidence of seroma was significantly higher in Group B (18.03%) than in Group A (5.26%) and Group C (4.69%) (P = 0.035). Multivariable logistic regression further identified the surgical technique used in Group B as an independent risk factor for seroma (aOR: 5.47, 95% CI: 1.35-22.13, P = 0.017). The incidences of scrotal hematoma and ischemic orchitis were significantly higher in Group A than in the other two groups (P = 0.012 for both). There were no significant differences among the three groups in postoperative VAS scores, intraoperative blood loss, urinary retention, infection, recurrence, or chronic pain rates. In this study, midline transection of the lateral hernia sac combined with lateral stump fixation was associated with a lower incidence of seroma after TAPP for large indirect inguinal hernias, without a significant increase in the risks of postoperative pain, scrotal hematoma, ischemic orchitis, infection, recurrence, or other complications.

Lung adenocarcinoma (LUAD) is characterized by insidious onset and early metastatic propensity. It is urgent to identify novel therapeutic agents with potent antitumor efficacy and low toxic side effects. This study aims to investigate the mechanism by which compound mylabris capsules (CMC) regulates ferroptosis in LUAD and identify its active ingredients through integrated bioinformatics analysis and experimental validation. The TCGA and GEO datasets related to LUAD were downloaded, and LUAD target genes were identified based on differentially expressed genes (DEGs) and weighted gene co-expression network analysis. Ferroptosis-related genes (FRGs) were obtained from the Genecards and FerrDb V2 databases, and the intersection with LUAD target genes yielded the LUAD-FRGs intersection genes. A drug-active ingredient-target network was constructed to screen the active ingredients and targets of CMC. Common targets were obtained by taking the intersection of CMC targets and LUAD-FRGs intersection genes, and enrichment analysis was further performed. In vitro experiments were conducted to verify the effects of main active ingredients on A549 and H1650 cells. Single-cell RNA sequencing data were used to analyze the expression and distribution of common targets. Key genes were screened using Lasso regression, Boruta, and RFE algorithms, and the expression levels, diagnostic, and prognostic values of key genes were further analyzed. Molecular docking and molecular dynamics simulation were performed to validate the interactions between the main active ingredients and key genes. 2508 LUAD target genes were obtained after intersecting 6699 DEGs with 3829 key module genes. Further intersection of 1599 FRGs with LUAD target genes, 247 LUAD-FRGs intersection genes were identified. A total of 1074 CMC targets and the top 3 active ingredients (quercetin, kaempferol, and β-sitosterol) were screened out. The intersection of CMC targets and LUAD-FRGs intersection genes yielded 31 common targets. Enrichment analysis demonstrated their significant involvement in biological processes such as the cell cycle, HIF-1 signaling pathway, VEGF signaling pathway, cellular senescence, and p53 signaling pathway. In vitro experiments showed that quercetin, kaempferol, and β-sitosterol exerted inhibitory effects on A549 and H1650 cells. Single-cell RNA sequencing data analysis indicated that common targets were predominantly expressed in the tumor cell subset. Machine learning identified CA9, EZH2, NQO1, and TERT as key genes, with their expression levels significantly elevated in the Tumor group. Additionally, ROC and univariate COX regression analyses suggested that these key genes possessed good diagnostic and prognostic values. Molecular docking and molecular dynamics simulation confirmed strong binding ability and stability between the main active ingredients and key genes. CMC exerts antitumor effects in LUAD by regulating ferroptosis-related targets such as CA9, EZH2, NQO1, and TERT. This study reported for the first time that CMC alleviated LUAD by regulating ferroptosis.