Fundación Pethema

Abstract The vegetarian and vegan pet foods are on the rise due to societal concerns towards environmental sustainability and livestock welfare. The nutritional properties of these pet foods with plant-based (PB) protein sources may be different from conventional foods with animal-based (AB) protein sources. For example, plant protein sources can contain fibers and anti-nutrients like phytate and polyphenols that can bind with mineral ions and form insoluble complexes, which reduces the availability of minerals for intestinal absorption. and prevent their availability. Our objective was therefore to advance our understanding of mineral bioavailability in vegetarian and vegan pet foods. An inventory was made of commercial vegetarian and vegan dry extruded dog foods using Google as search engine. The form (entire or processed into concentrate or isolate) of the first protein source(s) to be listed were noted. Based on product availability in the web shops, fifteen vegetarian and vegan dry extruded foods were purchased. Seven popular conventional dog foods were selected as reference products. Foods were analyzed for phytate (myo-inositol hexaphosphate) contents. Foods were subjected to in vitro simulation of gastric and small intestinal digestion. Foods and their insoluble residues collected after digestion were analyzed for dry matter and mineral contents (Ca, K, Mg, Zn, Mn, Fe, Cu, S, Se) to allow calculation of mineral (in)accessibility. Differences in mineral accessibility (%) between AB and PB foods were evaluated by one-way ANOVA and, for Ca and Mg, with a Mann-Whitney U test. Correlations between phytate content (g/g) and mineral accessibility (%) and phytate content and inaccessible minerals (g/g) were evaluated. PB dog foods contained more phytate (average 0.61, range 0.21-1.31 g/100 g) than in AB foods (0.28, 0.11-0.50 g/100 g) (P=0.014). Foods differed in in vitro mineral accessibility with PB foods having lower Mn, Fe, Ca, K, Mg accessibility (P< 0.05) but higher Cu accessibility (P< 0.05) and similar accessibility values for Zn, S and Se (P >0.10) compared to AB foods. No significant correlations were observed between phytate content and mineral accessibility (P >0.05). Phytate content correlated only with inaccessible Mg content. The total Mg content in the food was, however, also significantly correlated with phytate content. In conclusion, mineral accessibility of commercial PB extruded dog foods is generally lower than those in conventional AB foods. Mineral contents in these foods require more attention when industry guidelines are to be followed. Phytate contents did not explain variation in mineral accessibility of foods, which suggests that other factors like mineral source, fibers and polyphenols may play a role.

Next-Generation Sequencing is needed for the accurate genetic risk stratification of acute myeloid leukemia according to European LeukemiaNet (ELN) guidelines. We validated and compared the 2022 ELN risk classification in a real-life cohort of 546 intensively and 379 non-intensively treated patients. Among fit patients, those aged ≥65 years old showed worse OS than younger regardless risk classification. Compared with the 2017 classification, 14.5% of fit patients changed the risk with the 2022 classification, increasing the high-risk group from 44.3% to 51.8%. 3.7% and 0.9% FLT3-ITD mutated patients were removed from the favorable and adverse 2017 categories respectively to 2022 intermediate risk group. We suggest that midostaurin therapy could be a predictor for 3 years OS (85.2% with vs. 54.8% without midostaurin, P = 0.04). Forty-seven (8.6%) patients from the 2017 intermediate group were assigned to the 2022 adverse-risk group as they harbored myelodysplasia (MDS)-related mutations. Patients with one MDS-related mutation did not reach median OS, while patients with ≥2 mutations had 13.6 months median OS (P = 0.002). Patients with TP53 ± complex karyotype or inv(3) had a dismal prognosis (7.1 months median OS). We validate the prognostic utility of the 2022 ELN classification in a real-life setting providing supportive evidences to improve risk stratification guidelines.

Children frequently develop fever after hematopoietic stem cell transplant (HSCT). Although the etiology of many febrile episodes (FEs) is not an infection, patients often receive broad-spectrum antibiotics in response. To improve the judicious use of antibiotics in pediatric HSCT patients, we performed a prospective cohort study of children receiving an HSCT in Clínica Imbanaco (Cali, Colombia) between September 2016 and December 2019. We assessed all FEs occurring during 3 periods (infusion, neutropenic and engraftment). We measured procalcitonin and C-reactive protein (CRP) sequentially during each FE and compared levels among patients with fever due to significant infection (FSI) versus fever not attributable to infection (FNI) in each transplant period. There were 166 FEs in 95 patients. FSI accounted for 12%, 42% and 42% of FE during infusion, neutropenic and engraftment periods, respectively. CRP had better discriminatory capacity for FSI versus FNI in the infusion period [area under the curve (AUC) 0.80 (95% confidence interval [CI], 0.62-0.96) for a CRP level of 50 mg/L]. Neither biomarker performed well in the neutropenic period. During the engraftment period, a CRP of 65 mg/L had an AUC of 0.81 (95% CI, 0.65-0.96), while a procalcitonin level of 0.25 ng/mL had an AUC of 0.83 (95% CI, 0.63-1.0). In contrast to procalcitonin, the CRP's pattern of change throughout the first 3 days of fever in each transplant period was different in FSI compared with FNI. Sequential measurement of biomarkers, especially CRP, may allow clinicians to more appropriately manage antibiotic use in pediatric HSCT units.

Indoor positioning and navigation systems are getting popular nowadays. There are different types of products in the way of accuracy, cost and power consumption in the field. Especially in the last couple of years, RSSI (Received Signal Strength Indicator) based positioning algorithms have studied but the results are not sufficient and there is no exact way decided to overcome this problem. In this paper, we will explain a method that combines Deep Learning and BLE (Bluetooth Low Energy) Fingerprinting method to get better accurate results.

Dalam rangka mewujudkan amanat nawacita dibutuhkan Peta Desa sebagai rujukan bagi Kementrian/Lembaga serta Pemerintah Daerah dalam program pembangunan. Peta desa adalah peta tematik bersifat dasar yang berisi unsur dan informasi batas wilayah, infrastruktur transportasi, toponim, perairan, sarana prasarana, penutup lahan dan penggunaan lahan yang disajikan dalam peta citra, peta sarana dan prasarana, serta peta penutup lahan dan penggunaan lahan. Hal tersebut sesuai dengan UU Nomor 6 Tahun 2016 tentang Desa, yang menjelaskan bahwa Desa merupakan subjek dari pembangunan. Peraturan Kepala BIG Nomor 3 Tahun 2016 tentang Spesifikasi Teknis Penyajian Peta Desa merupakan peraturan yang diterbitkan oleh Badan Informasi Geospasial untuk mengatur pembuatan sebuah Peta Desa. Dengan terbitnya aturan tersebut maka dilakukan analisa terhadap Peta Desa Beran yang digunakan sebagai media peningkatan status dari desa menjadi kelurahan. Analisa dilakukan terhadap ukuran muka peta, interval grid peta, spesifikasi tata letak, pewarnaan simbol peta, spesifikasi penulisan informasi peta, dan keefektifan simbol yang digunakan pada Peta Desa Beran, Kabupaten Ngawi. Hasil penelitian ini menunjukkan nilai ukuran muka peta dan susunan/spesifikasi tata letak informasi peta desa yang digunakan oleh Peta Desa Beran masih belum sesuai dengan Peraturan Kepala BIG Nomor 3 Tahun 2016 tentang Spesifikasi Teknis Penyajian Peta Desa. Terdapat pula tiga objek di lapangan yang simbolnya tidak diatur dalam Peraturan Kepala BIG Nomor 3 Tahun 2016 tentang Spesifikasi Teknis Penyajian Peta Desa, yaitu Yayasan, Panti, dan Koperasi Simpan Pinjam (KSP). Selain itu simbol yang ditampilkan pada Peta Desa Beran sebagai pengganti objek yang ada di lapangan terlalu banyak, dikarenakan tidak semua objek pada simbol tersebut dimiliki oleh Desa Beran.