Grains Research and Development Corporation

The role of nurse educators in academia requires balancing teaching, research, and administrative responsibilities, often with limited institutional recognition or support. These demands can influence job satisfaction, professional relationships and faculty retention. In Croatia, where nursing education is still developing within the higher education system, little is known about the experiences of nurse educators. This study explored job satisfaction and professional relationships of nurse educators employed full time in higher education institutions in Croatia. An exploratory descriptive qualitative study was conducted in 2024 among full-time nurse educators from 12 higher education institutions offering accredited nursing programs in Croatia. Data were collected through semi-structured online interviews. The interview guide included questions on job satisfaction and professional relationships. Data were analysed using reflexive thematic analysis. The study included 52 participants. Participants generally reported high job satisfaction, associated with autonomy in academic work, opportunities for professional development, and meaningful interaction with students. However, dissatisfaction stemmed from administrative overload, limited financial incentives, and uneven task distribution among colleagues. Institutional support and collegiality emerged as key factors sustaining motivation and professional identity, while many educators expressed a desire for closer integration of academic and clinical roles to strengthen both teaching relevance and clinical competence. This study highlighted the complex interplay between job satisfaction, professional expectations, and institutional support of nurse educators employed as full-time faculty. The findings emphasise the need for institutional and policy reforms to enhance the working conditions and recognition of nurse educators. Reducing administrative demands, providing fair financial and professional rewards, and establishing structured mentorship and development programs could foster career sustainability and faculty retention. Strengthening institutional support for academic nurses is essential for maintaining a competent and motivated teaching workforce, ultimately improving the quality and continuity of nursing education and practice in Croatia. Not applicable. This study was not a clinical trial.

Mlade djevojke, adolescentice u razdoblju puberteta izložene su brojnim izazovima, a jedan od njih je zasigurno maloljetna i neželjena trudnoća, na koju djevojke u toj razvojnoj dobi najčešće nisu spremne. Cilj rada je osvijestiti važnost edukacije, podrške i sustavnih promjena u rješavanju problema maloljetne i neželjene trudnoće. Maloljetna trudnoća se odnosi na trudnoću u kojoj trudnica nije punoljetna, što može varirati ovisno o državi, no obično podrazumijeva 18 godina. Neadekvatan, odnosno nedovoljno prisutan seksualni odgoj u školama postavlja se kao značajan problem kod maloljetne i neželjene trudnoće. Također, nedostupnost kontracepcije, što zbog financijskih razloga što zbog stigmatizacije, značajno pridonosi ovoj problematici. Nadalje, u društvima gdje su teme seksualnog zdravlja tabuizirane, očekivana je nedovoljna briga o reproduktivnom zdravlju žene. Važno je izbjeći svaki oblik stigmatizacije neželjene ili maloljetne trudnoće, kako osoba ne bi osjećala sram i dodatno narušila psihičko zdravlje. Naglasak prvenstveno treba biti na prevenciji, promociji seksualnog, zdravstvenog odgoja u školama, dostupnosti kontracepcije, te edukaciji mladih ljudi. Kada do neželjene, odnosno maloljetne trudnoće ipak dođe, ključna i prijeko potrebna je emocionalna, psihološka pomoć majci, koja se nosi sa složenom, novonastalom situacijom, te brojnim izazovima i emocijama u mladoj dobi.

Abstract Kochia [Bassia scoparia (L.) A.J. Scott] is an invasive species in the High Plains of the United States that poses formidable management challenges in agricultural systems, primarily due to its evolution of resistance to glyphosate. Resistance is due to a transposon-associated increase in 5-enolpyruvyl-3-shikimate phosphate synthase (EPSPS) gene copy number relative to the sensitive biotype. Factors behind the rapid spread of glyphosate-resistant biotypes are likely associated with certain aspects of B. scoparia biology, such as a protogynous flower morphology producing large amounts of pollen, that encourages outcrossing and favors high genetic diversity. Furthermore, its ability to tumble over long distances ensures a rapid spread of the resistance trait. Herein, we explore glyphosate resistance in B. scoparia in Colorado. There was no difference in EPSPS gene (Type I, Type II) and FAR1 copy numbers between parent and progeny B. scoparia populations across multiple years (2018, 2020, and 2022), suggesting stable inheritance of glyphosate resistance. Further, the inheritance of glyphosate resistance was investigated using three specific microsatellites or simple sequence repeat (SSR) markers viz. 2656, 2896, and 1792. SSR marker analysis revealed an outcrossing rate of 78% and a selfing rate of 22% in B. scoparia progeny. By investigating the complex interplay between B. scoparia’s biology and genetics, this study investigates the inheritance of glyphosate resistance in B. scoparia, estimates the outcrossing rate under field conditions, and underscores the importance of developing effective management strategies to mitigate its impact on agricultural ecosystems.

Environmental change requires more crop production per water use to meet the rising global food demands. However, improving crop intrinsic water use efficiency (iWUE) usually comes at the expense of carbon assimilation. Sorghum is a key crop in many vulnerable agricultural systems with higher tolerance to water stress (WS) than most widely planted crops. To investigate physiological controls on iWUE and its inheritance in sorghum, we screened 89 genotypes selected based on inherited haplotypes from an elite line or five exotics lines, containing a mix of geographical origins and dry versus milder climates, which included different aquaporin (AQP) alleles. We found significant variation among key highly heritable gas exchange and hydraulic traits, with some being significantly affected by variation in haplotypes among parental lines. Plants with a higher proportion of the non-stomatal component of iWUE still maintained iWUE under WS by maintaining photosynthetic capacity, independently of reduction in leaf hydraulic conductance. Haplotypes associated with two AQPs (SbPIP1.1 and SbTIP3.2) influenced iWUE and related traits. These findings expand the range of traits that bridge the trade-off between iWUE and productivity in C4 crops, and provide possible genetic regions that can be targeted for breeding.

Five grapevine viruses, including grapevine geminivirus A (GGVA), grapevine Syrah virus 1 (GSyV-1), grapevine Cabernet Sauvignon reovirus (GCSV), grapevine virus F (GVF) and grapevine Red Globe virus (GRGV) have been detected in four South Australian vineyards using metagenomic high throughput sequencing for the first time in Australia. Most of them were present in asymptomatic grapevines and some were present in grapevines with leafroll disease that were infected by several other viruses, including grapevine leafroll-associated viruses. Therefore, the association of GGVA, GSyV-1, GCSV, GVF and GRGV with disease in Australia is unknown.

Accelerating genetic gain in crop improvement is required to ensure improved yield and yield stability under increasingly challenging climatic conditions. This case study demonstrates the effective confluence of innovative breeding technologies within a collaborative breeding framework to develop and rapidly introgress imidazolinone Group 2 herbicide tolerance into an adapted Australian chickpea genetic background. A well-adapted, high-yielding desi cultivar PBA HatTrick was treated with ethyl methanesulfonate to generate mutations in the ACETOHYDROXYACID SYNTHASE 1 (CaAHAS1) gene. After 2 years of field screening with imidazolinone herbicide across >20 ha and controlled environment progeny screening, two selections were identified which exhibited putative herbicide tolerance. Both selections contained the same single amino acid substitution, from alanine to valine at position 205 (A205V) in the AHAS1 protein, and KASP™ markers were developed to discriminate between tolerant and intolerant genotypes. A pipeline combining conventional crossing and F2 production with accelerated single seed descent from F2:4 and marker-assisted selection at F2 rapidly introgressed the herbicide tolerance trait from one of the mutant selections, D15PAHI002, into PBA Seamer, a desi cultivar adapted to Australian cropping areas. Field evaluation of the derivatives of the D15PAHI002 × PBA Seamer cross was analyzed using a factor analytic mixed model statistical approach designed to accommodate low seed numbers resulting from accelerated single seed descent. To further accelerate trait introgression, field evaluation trials were undertaken concurrent with crop safety testing trials. In 2020, 4 years after the initial cross, an advanced line selection CBA2061, bearing acetohydroxyacid synthase (AHAS) inhibitor tolerance and agronomic and disease resistance traits comparable to parent PBA Seamer, was entered into Australian National Variety Trials as a precursor to cultivar registration. The combination of cross-institutional collaboration and the application of novel pre-breeding platforms and statistical technologies facilitated a 3-year saving compared to a traditional breeding approach. This breeding pipeline can be used as a model to accelerate genetic gain in other self-pollinating species, particularly food legumes.

Granular materials are comprised of solid, athermal grains. Whilst immune to thermal motion, these grains move and diffuse when they undergo shear deformation. Here we introduce this process of shear-induced diffusion with a focus on dense flows. The goal is to present the established scaling laws for continuum diffusivity and to relate them to the micro-mechanisms of a granular random walk. We then suggest how this knowledge may help advance our understanding of granular rheology and diffusion in other soft-materials.

Blackleg is a worldwide disease of canola (Brassica napus), caused by a complex of fungal species in the genus Leptosphaeria, that impacts canola production and seed quality. Demethylation inhibitor (DMI) fungicides that target sterol 14α-demethylase are an integral part of disease control. Here, we report six DMI-resistant isolates of Leptosphaeria maculans and two different types of genetic modification related to the resistance. Analysis of the regulatory region of the DMI target gene ERG11 (also known as CYP51) revealed a 275-bp insertion in two of the isolates and three long terminal repeat retrotransposons (5,263, 5,267, and 5,248 bp) inserted in the promoter region of three resistant isolates. Genetic approaches confirmed that these elements are responsible for DMI resistance in L. maculans and crosses show segregation consistent with a single locus. Reverse-transcription quantitative PCR assays demonstrated that the 275-bp insertion increases ERG11 gene expression, conferring DMI fungicide resistance both in vitro and in planta. Moreover, transformation of a susceptible isolate of L. maculans with ERG11 driven by a promoter containing the 275-bp insertion increased resistance to tebuconazole. A minimal shift of the values of concentration whereby 50% of the mycelial growth is inhibited in vitro was observed in resistant isolates containing long terminal repeat retrotransposons; nevertheless, these isolates were able to develop significant lesions on cotyledons from fungicide-treated seedlings. This is the first report of genetic modifications in L. maculans relating to DMI fungicide resistance.