Hochschule der Bildenden Künste Saar

Ascorbate peroxidase (APEX) is a proximity labeling enzyme used for subcellular proteomics at high spatial and temporal resolution. However, toxicity of its substrate hydrogen peroxide and background labeling by endogenous peroxidases limit its use to in vitro studies of specific cell types. To minimize toxicity and reduce non-specific background labeling we establish a more versatile in situ APEX activation (iAPEX) workflow by combining APEX2 with a D-amino acid oxidase to locally produce hydrogen peroxide. Using iAPEX, we profile the proteomes of a cellular microdomain, the primary cilium, in cell lines not readily accessible to conventional APEX labeling and identify unknown ciliary proteins. Our study validates common ciliary proteins across two distinct cell lines, while observed differences may reflect heterogeneity in primary cilia proteomes. Furthermore, iAPEX proximity labeling is applicable to a range of cellular compartments including mitochondria and lipid droplets and can be employed in Xenopus laevis, which provides a proof-of-concept for future in vivo applications.

This paper introduces a hybrid dual-path architecture that bridges the gap between interpretable lexicon-based methods and opaque LLMs by integrating a contextual encoder (RoBERTa-MLP) with a graph-based encoder (GAT-eMFD) that captures structured mor

Virtual reality (VR) technology provides clinicians, therapists, and researchers with new opportunities to observe, assess, and train behavior in realistic yet well-controlled environments. However, VR also comes with a number of challenges. For example, compared to more abstract experiments and tests on 2D computer screens, VR-based tasks are more complex to create, which can make it more expensive and time-consuming. One way to overcome these challenges is to create, standardize, and validate VR content and to make it openly available for researchers and clinicians. Here we introduce the OpenVirtualObjects (OVO), a set of 124 realistic 3D household objects that people encounter and use in their everyday lives. The objects were rated by 34 younger and 25 older adults for recognizability, familiarity, details (i.e., visual complexity), contact, and usage (i.e., frequency of usage in daily life). All participants also named and categorized the objects. We provide the data and the experiment- and analysis code online. With OVO, we hope to facilitate VR-based research and clinical applications. Easy and free availability of standardized and validated 3D objects can support systematic VR-based studies and the development of VR-based diagnostics and therapeutic tools.

To deepen the engagement of exhibition visitors with cultural heritage, we develop an interactive design approach to storytelling in which users can act both as co-curators and co-authors through a multitouch interface.

This chapter describes the importance and integration of innovation in today’s art and design education, leading to radical changes in the curricula of the universities of fine arts. Modularization replaces not only the old system of master classes but opens the individual choices of subjects to a system of projects. Adding to this, the installation of nonconsecutive curricula integrating science and art as well as co-operative curricula between classical universities and art institutions offer new horizons in both creative as traditional industries and sciences. Another step is the installation of In- and At-House-Institutes acting as professional links between education, management and politics. All of these elements that have been developed within the past decade are described on the example of the Saar University of Fine Arts (HBKsaar) which was the first Fine Art University in Germany to be fully modularized. Creativity has, at last, gained high importance as a key issue of industrial and economic development, and the professional education in this field will acknowledge the creative entrepreneurship in both arts and design.