Kazakh Research Institute of Agriculture and Plant growing

This study evaluates the effects of organo-mineral fertilization systems combined with biological preparations on the productivity and soil microbiological indicators of Golden Delicious apple trees in southeastern Kazakhstan. Using a randomized block design, six fertili-zation variants— including organic, bioorganic, and mineral treatments - were applied to irrigated light brown soils with low organic matter content. Results demonstrated that treatments with biohumus and BioSok Energy Plus significantly increased yields (39–64%,) sugar content (0.5–1.9%), dry matter (0.8–1.5%), and fruit shelf life compared to controls. Additionally, these treat-ments enhanced the abundance of beneficial soil microorganisms, indicating improved soil biological activity and structure. The findings suggest that integrating organic and bioorganic fertilizers with mineral nutrition promotes sustainable orchard productivity by boosting soil fertility and microbiocenosis, thereby supporting long-term soil health and sustainable horticultural practices in arid regions.

Diseases caused by Bipolaris sorokiniana, expressed as leaf spot blotch (SB) and common root rot (CRR), continue to limit spring wheat production, particularly in dry regions where yield losses may reach 35-40%. This study evaluated resistance to SB and CRR in fifty spring wheat genotypes at both seedling and adult plant stages and identified genetic sources of resistance using molecular markers linked to the Sb1 and Sb2 genes. Field trials were conducted in 2023 and 2024 in the Aktobe region under natural infection, artificial inoculation, and a fungicide-treated background. Based on leaf spot blotch severity quantified as the area under the disease progress curve (leaf AUDPC) under natural infection, nine genotypes displayed stable resistance across both years, while fungicide-treated plots revealed twenty-three resistant genotypes in 2023 and eighteen in 2024. Artificial inoculation identified five resistant lines in 2023 and one in 2024. Resistance to common root rot (CRR) was assessed independently based on subcrown internode (SCI) browning at the adult plant stage. Seedling assays confirmed consistent resistance in six genotypes, all of which carried Sb1, Sb2, or their combination. In total, Sb genes were detected in thirty-six of the fifty accessions, including genotypes from Kazakhstan, Russia, and several other countries. The presence of Sb1 or Sb2 was associated with reduced disease severity, particularly at the seedling stage. These findings identify valuable germplasm for breeding wheat with improved resistance to B. sorokiniana in Kazakhstan.

Soil contamination with heavy metals is a persistent challenge in the arid regions of Kazakhstan. This study evaluates the phytoremediation potential of sweet sorghum (Sorghum bicolor L.), a drought-tolerant crop with a well-developed root system, using a combination of in vitro and analytical approaches. In vitro culture of somatic cells revealed clear genotype-dependent differences in callus induction and morphogenesis, with Hybrid-2 and SAB-3 exhibiting the highest regenerative capacity and thus the greatest suitability for further biotechnological improvement and stress-tolerance selection. Analysis of metal distribution, based on atomic absorption spectroscopy (AAS), demonstrated that S. bicolor predominantly retained Pb, Cd, and Co in the root system. Cobalt accumulated to 12.7 ± 1.32 mg/kg under 1 MAC and 16.87 ± 2.78 mg/kg under 2 MAC, accounting for more than half of the metal absorbed by plants. Cadmium showed a similar root-dominant pattern, whereas lead exhibited the lowest mobility and remained almost entirely sequestered in roots, with translocation factors consistently below unity (TF < 1). Overall, these findings confirm the suitability of sweet sorghum as an environmentally sustainable species for the phytostabilization of Pb-, Cd-, and Co-contaminated soils in arid environments and highlight the value of genotype pre-selection under stress conditions for optimizing phytoremediation performance.

In the context of growing interest in functional nutrition, enriching food products with naturally active compounds is an important task. This research aimed to develop extruded cereal crackers enriched with concentrates from wild plants in Kazakhstan, such as hawthorn (Crataegus laevigata), chokeberry, rosehip, and sea buckthorn. The concentrates were obtained by ultrasound-assisted extraction and concentrated using vacuum evaporation. Wheat and buckwheat crispbreads were prepared by high-temperature extrusion. Afterwards, a plant-based syrup containing the concentrates was applied. This was a controlled experimental study. Samples were prepared using a fixed recipe and analyzed for bioflavonoid content and quality indicators. The bioactive composition was evaluated in the accredited laboratory of Nutritest LLP according to the methodology Р 4.1.1672-2003. The highest content of rutin (157.8 ± 7.9 mg/100 g) was observed in hawthorn samples, dihydroquercetin (181.93 ± 9.10 mg/100 g) in chokeberry samples, and chlorogenic acid (42.75 ± 2.14 mg/100 g) in sea buckthorn samples. All samples met physicochemical standards (moisture, acidity, ash content). The study confirmed the high antioxidant activity and potential of wild plants for the development of functional foods. These products have attractive sensory characteristics and are promising for use in preventive nutrition.

Kazakhstan, a fossil-fuel-dependent economy, faces growing pressure to reduce greenhouse gas emissions while maintaining industrial competitiveness. Carbon Capture, Utilization, and Storage (CCS/CCUS) technologies offer a viable pathway for decarbonizing hard-to-abate sectors, particularly in power generation, metallurgy, and oil and gas processing. This paper provides a comprehensive review of the state of CCS/CCUS technologies globally and examines their applicability within Kazakhstan. The study also explores long-term CO2 storage mechanisms and monitoring frameworks, with attention to carbon leakage risks and the importance of addressing methane emissions. A critical part of the analysis is dedicated to Kazakhstan’s Emissions Trading System, identifying its current limitations such as low carbon prices, and limited sectoral coverage, and outlining practical reforms to enhance its role in supporting CCS/CCUS and broader decarbonization efforts. The integration of CCS/CCUS with a strengthened ETS, combined with access to international climate finance instruments and voluntary carbon markets, is proposed as a key strategy for Kazakhstan’s transition to a low-carbon economy. By linking engineering innovation with targeted policy interventions, this study offers a dual-perspective contribution. It not only provides technical insights into CCS/CCUS technologies but also presents policy recommendations that are specifically tailored to Kazakhstan’s context. The findings reinforce the role of CCS/CCUS as a crucial component of national climate strategy and industrial transformation.

Germination is a biotechnological process that activates enzymatic reactions and alters the chemical content of grain, improving its nutritional and functional value. However, the duration of the germination process significantly affects grain composition and properties. Prolonged and uncontrolled germination can lead to undesirable changes, such as excessive enzymatic activity, microbial contamination of sprouted grains, and loss of dry matter. While short-term germination is preferable for producing various food products, including whole-grain porridge, this article analyzed the impact of short-term germination of triticale grain (for 18, 20, 22, and 24 h) on its biochemical indicators and functional-technological properties. This research established that changes in biochemical values accompany the germination of triticale grain. The most significant increase in protein content was observed after 20 h of germination (GT 20), which increased by 4.43%. In contrast, after 24 h (GT 24), a decrease of 3.27% was noted compared to the original content in the whole grain (WGT). The contents of fats, carbohydrates, and ash tended to decrease. Germination promoted an increase in the amount of essential amino acids and improved the amino acid profile, particularly during the 20 and 22 h germination intervals. At 24 h of germination, the highest increase in total phenolic compounds (28.6%) and antioxidant activity (20.8%) was recorded. B-group vitamins, thiamine, and riboflavin, were detected in the sprouts only after 22 and 24 h of germination. The highest thiamine content (0.17 mg/kg) was observed at the 22nd hour, and the highest riboflavin content (2.51 mg/kg) was observed at the 24th hour. Niacin content showed a steady increase throughout the germination period. The maximum magnesium (0.200%) and molybdenum (0.589%) contents were recorded after 24 h of germination, while the calcium content increased at all germination intervals. The functional and technological properties of sprouted triticale grain improved, while the pasting (gelatinization) properties tended to decrease. Thus, it has been established that short-term germination enhances the biochemical indicators and functional-technological properties of triticale grains, indicating their potential for use in the production of whole-grain porridge.

Winter wheat is considered one of the sources of increased grain production according to higher yield and better use of bioclimatic resources in Western Siberia. The objective of this study was to breeding evaluate and identify genetically distinct genotypes among an international collection of winter bread wheat accessions for potential use as parents and for the development of high-yielding varieties resistant to adverse environmental factors. Materials and methods. The breeding evaluation of 96 accessions of winter wheat generated from different breeding programs was performed in 2022 and 2023, using generally accepted agronomy techniques. All 96 accessions were genotyped using 55 KASP markers developed in Institute of Plant Biology and Biotechnology (Kazakhstan). Results. The accessions from Turkey had a longer growing period. The accessions from the USA were characterized by short stature (57.5 cm), more spikes on plants (2.42), and increased yield (271 g/m2). The samples from Russia and Bulgaria were distinguished by high levels of winter hardiness (66.7% and 69.9%, respectively). The average number of alleles was 2, and the effective number of alleles was 1.42. The analysis of the genotypic structure of the population determined the division of genotypes into 6 subclusters regardless of their origin. Shannon’s information index ranged from 0.058 to 0.693, with an average value of 0.40 and an expected heterozygosity of 0.25, which indicated a relatively high genetic diversity of the studied accessions. Conclusion. The results of this research can be successfully used in breeding to develop high-yielding winter wheat varieties, that are resistant to adverse environmental factors and have high grain quality. Acknowledgments. Field experiments were carried out with the support of the Russian Science Foundation (agreement No. 23-16-20006 dated April 20, 2023). EDN: LMBBIY

This study evaluated 290 recombinant inbred lines (RILs) of the nested association mapping (NAM) population from the UK. The population derived from 24 families, where a common parent was "Paragon," one of the UK's spring wheat cultivar standards. All genotypes were tested in two regions of Kazakhstan at the Kazakh Research Institute of Agriculture and Plant Industry (KRIAPI, Almaty region, Southeast Kazakhstan, 2019-2022 years) and Alexandr Barayev Scientific-Production Center for Grain Farming (SPCGF, Shortandy, Akmola region, Northern Kazakhstan, 2019-2022 years). The studied traits consisted of plant adaptation-related traits, including heading date (HD, days), seed maturation date (SMD, days), plant height (PH, cm), and peduncle length (PL, cm). In addition, the yield per m2 was analyzed in both regions. Based on a field evaluation of the population in northern and southeastern Kazakhstan and using 10,448 polymorphic SNP (single-nucleotide polymorphism) markers, the genome-wide association study (GWAS) allowed for detecting 74 QTLs in four studied agronomic traits (HD, SMD, PH, and PL). The literature survey suggested that 16 of the 74 QTLs identified in our study had also been detected in previous QTL mapping studies and GWASs for all studied traits. The results will be used for further studies related to the adaptation and productivity of wheat in breeding projects for higher grain productivity.

Soybean [Glycine max (L.) Merr.] is one of the important crops that are constantly increasing their cultivation area in Kazakhstan. It is particularly significant in the southeastern regions of the country, which are currently predominant areas for cultivating this crop. One negative trait reducing yield in these dry areas is pod dehiscence (PD). Therefore, it is essential to understand the genetic control of PD to breed new cultivars with high yield potential. In this study, we evaluated 273 soybean accessions from different regions of the world for PD resistance in the conditions of southeastern regions of Kazakhstan in 2019 and 2021. The field data for PD suggested that 12 accessions were susceptible to PD in both studied years, and 32 accessions, in one of the two studied years. The genotyping of the collection using a DNA marker for the Pdh1 gene, a major gene for PD, revealed that 244 accessions had the homozygous R (resistant) allele, 14 had the homozygous S (susceptible) allele, and 15 accessions showed heterozygosity. To identify additional quantitative trait loci (QTLs), we applied an association mapping study using a 6K SNP Illumina iSelect array. The results suggested that in addition to major QTL on chromosome 16, linked to the physical location of Pdh1, two minor QTLs were identified on chromosomes 10 and 13. Both minor QTLs for PD were associated with calmodulin-binding protein, which presumably plays an important role in regulating PD in dry areas. Thus, the current study provided additional insight into PD regulation in soybean. The identified QTLs for PD can be efficiently employed in breeding for high-yield soybean cultivars.