Laboratoire de Recherche et d’Analyses Médicales de la Fraternelle de la Gendarmerie Royale

This study intended to examine and assess the performance of raw and treated Eriobotrya Japonica seed waste for the adsorption-based removal of methylene blue dye from an aqueous solution. The effects of several factors, including pH, adsorbent dose, and initial concentration, on the elimination of this dye were examined. To optimize the removal process, a Box-Behnken design (BBD) based on response surface methodology (RSM) was employed to evaluate the influence of key variables, adsorbent dose, initial dye concentration, and pH, along with their interactions. The findings demonstrated that the statistical analysis reveals a high significance of the dye for raw and treated Eriobotrya Japonica seed waste, with extremely weak probability values (p < 0.0001). The optimal conditions achieved were the adsorbent dose = 21.21 mg, initial dye concentration = 7.54 mg/L, and pH = 10.92 for the raw waste and adsorbent dosage = 21.75 mg, initial dye concentration = 7.5 mg/L, and pH = 11.7 for the extracted waste. These conditions result in a dye removal efficiency of 99.48% and 99.88% for raw and treated Eriobotrya Japonica seed waste, respectively. The methylene blue adsorption kinetics on the adsorbent can be precisely represented by an effective pseudo-second-order equation. The Freundlich model showed a significantly better fit to the experimental results compared to the Langmuir model.

Nowadays, reverse osmosis (RO) desalination has become a highly effective and economical solution to address water scarcity worldwide. The membranes used in this type of separation are influenced by both pre-treatment operations and feed water quality, leading to fouling, a complex phenomenon responsible for reducing treatment performance and shortening membrane lifespan. In this study, an autopsy of a RO membrane from the Boujdour plant was performed, and a fouling prediction tool based on transmembrane pressure (TMP) was developed using MATLAB/Simulink (R2015a) with an artificial neural network (ANN) model. The impact of membrane fouling on treatment performance was also examined through one year of monitoring. A detailed analysis of the fouled membrane was conducted using SEM/EDS techniques to characterize the fouling on the membrane’s surface and cross-section. The results revealed significant fractures on the membrane surface, with fouling predominantly consisting of organic deposits (characterized by a high oxygen concentration of 39.69%) and inorganic fouling, including Si (7.99%), Al (2.79%), Mg (1.56%), Fe (1.27%), and smaller quantities of K (0.87%), S (0.36%), and Ca (0.12%). The ANN model for predicting transmembrane pressure was successfully developed, achieving a high R2 value of 92.077% and a low mean square error (MSE) of 0.005657. This predictive model demonstrates the ability to forecast future TMP cycles based on historical data. The research provides a detailed understanding of the types of fouling affecting RO membranes and contributes to the development of preventive strategies to mitigate membrane fouling.

Introduction: Cannabis sativa threshing residue is the plant material left after extracting resinous trichomes from the dried female plant. This study examines the phytochemical properties of C. sativa threshing residue and screens its vasorelaxant, antimicrobial, and immunomodulatory activities. Methods: The residue was extracted sequentially using hexane, dichloromethane, ethyl acetate, and ethanol. The four fractions were analyzed by gas chromatography-mass spectrometry. Phenolic and flavonoid contents were estimated using Folin-Ciocalteu reagent and aluminum chloride colorimetric methods. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay determined in vitro antioxidant activity. The vasorelaxant effect was explored using rat mesenteric arterial beds (MABs). Antimicrobial studies on Salmonella enterica, Escherichia coli, Staphylococcus aureus, and Bacillus cereus were conducted using microdilution tests. Immunomodulatory activity was determined using cell proliferation, antibody production, and complement tests. Results: Phytochemical analysis of the four fractions revealed many volatile bioactive compounds, including terpenes like phytol and squalene and cannabinoids like delta-9-tetrahydrocannabinol and cannabidiol (CBD). The fractions had significant polyphenol and flavonoid content. They dose-dependently dilated the MAB and demonstrated varying DPPH free radical scavenging activity. Immunomodulatory activity showed an immunosuppressive effect on cell proliferation and biological functions. The four bacterial strains studied were sensitive to the fractions but with different degrees. Conclusion: Cannabis sativa threshing residue demonstrated diverse pharmacological potential, suggesting its suitability for developing cannabis-based medications targeting hypertension-related damage, free radicals, and bacteria, expanding its potential applications beyond inflammation-related conditions

The stability, sensitivity, and volatility of essential oils are some of their most serious limitations, and nanoencapsulation has been considered one of the most effective techniques for solving these problems. This research aimed to investigate the incorporation of Salvia rosmarinus Speen and Cedrus atlantica Manetti (MEO) essential oil mixture in Gum Arabic (GA) and to evaluate nanoencapsulation’s ability to promote antifungal activity against two brown rot fungi responsible for wood decay Gloeophyllum trabeum and Poria placenta. The optimization of encapsulation efficiency was performed using response surface methodology (RSM) with two parameters: solid-to-solid (MEO/GA ratio) and solid-to-liquid (MEO/ethanol). The recovered powder characterization was followed by various techniques using a scanning electron microscope (SEM), X-ray diffractometry (XRD), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), and thermo-gravimetric analysis (TGA). The optimal nanoencapsulating conditions obtained from RSM were ratios of MEO/GA of 1:10 (w/w) and MEO/ethanol of 10% (v/v), which provided the greatest encapsulation efficiency (87%). The results of SEM, XRD, DLS, FTIR, and TGA showed that the encapsulation of MEO using GA modified particle form and molecular structure and increased thermal stability. An antifungal activity assay indicated that an effective concentration of MEO had an inhibitory effect on brown rot fungi. It had 50% of the maximal effect (EC50) value of 5.15 ± 0.88 µg/mL and 12.63 ± 0.65 µg/mL for G. trabeum and P. placenta, respectively. Therefore, this product has a great potential as a natural wood preservative for sustainable construction and green building.

The complex demographic history of human populations in North Africa has resulted in a high degree of genetic heterogeneity across the region. However, little is known about the pattern of these genetic variations in its current populations. The present study provides new data on the genetic background of Chaouia, an Arabic-speaking North African population in the western center of Morocco. A random sample of 150 unrelated healthy individuals from Chaouia was assessed using the AmpFLSTR Identifiler kit. The most polymorphic markers were D21S11 and D18S51, with 23 and 22 alleles, respectively. After Bonferroni's correction, two loci (TH01 and D18S51) deviated from Hardy-Weinberg equilibrium. The phylogeny analysis separated North African populations into northeastern and northwestern groups. The Chaouia population was clustered with northwestern Africans. It was the closest to the Berbers of Azrou. The Chaouia shared close genetic affinities with populations from North Africa, the Middle East, and Europe, particularly Iberians, and to a lesser extent with sub-Saharan populations. The pattern of genetic admixture varied across North African populations without a clear correlation between their geographic (northeastern or northwestern) or linguistic identities (Arab or Berber), however, genetic heterogeneity among Berbers was observed. These findings suggest that the diversity observed in North African populations extends geographical and linguistic boundaries. It is further linked to each population's unique and complex demographic history. Human North African population genetics seems to present an intriguing landscape for future studies in the region and its surrounding populations to trace the origins of the genetic heterogeneity observed in these populations.

Abstract This work presents the results obtained during the simulation of the radiative effects resulting after a total rupture of a tank filled to a certain level with commercial butane under a certain pressure. The radiative flux is estimated from fireball Bleve which has a geometrical form resembling that of a mushroom. The results are calculated based on a proposed mathematical model, and then compared with those obtained by the dynamic forecasting model, considering a spherical geometry for the fireball. The expected interest of this comparison is to highlight the radiative flux emitted by the cylindrical part of the mushroom shape attended in various experimental observations that are often ignored. This comparison is carried out taking into account the distance of the target from the center of the spherical part of the mushroom structure, throughout the lifetime of the phenomenon, from rupture to disappearance.KeywordsBleveFireballMushroom shapeDynamic modelView factorRadiative fluxFroude number

Eggs constitute an important part of the Moroccan diet. However, contaminated eggs can cause a serious public health problem if consumed undercooked, uncooked, or used in unpasteurized egg foodstuffs. This study was carried out to evaluate the microbial contents of eggs according to their sales sector in Morocco. For that, a total of 1770 eggs were collected from January to September 2021 from formal markets (refrigerated eggs from large shopping centers) and informal markets (eggs at ambient temperature from ambulatory sellers, street vendors, kiosks, and neighborhood stores) and transferred to the Avian Pathology Unit at Hassan II Agronomic and Veterinary Institute. The eggshells and their contents were tested separately; swabs of eggshells were used to inoculate Mac-Conkey agar, while the egg contents were cultured on Mac-Conkey and Mannitol salt agar, then standard microbiological tests were performed to identify the isolated organisms. The results showed that informal eggs were more contaminated (87%) than formal eggs (48) (p < 0.05). The bacteria isolated from the eggshells (informal and formal) were Enterobacter agglomerans (59% and 21%), Klebsiella spp. (24% and 4%), Enterobacter cloacae (17% and 8%), E. coli (9% and 1%), Serratia spp. (9% and1%), Pseudomonas aeruginosa (9% and 1%), Shigella spp. (5% and 0%), Salmonella enteritidis (0% and 2%), Proteus spp. (4% and 0%), Enterobacter sakazakii (2% and 0%), Rahnella aquatilis (1% and 0%), and Staphylococcus aureus (0% and 1%). For the egg-contents, the detected bacteria (informal and formal) were Enterobacter agglomerans (14% and 28%), Klebsiella spp. (7% and 6%), Staphylococcus aureus (6% and 1%), Enterobacter cloacae (4% and 4%), E. coli (4%, 1%), Shigella spp. (4%, 0%), Acinetobacter baumannii (3% and 1%), Salmonella enteritidis (2% and 0%), Serratia spp. (1% and 6%), Proteus spp. (1% and 3%), and Enterobacter sakazakii (1% and 0%). We conclude that eggs might be contaminated with several bacteria and can constitute a public health threat in Morocco.