Mane (France)

Résumé Créé au Théâtre de la ville au printemps 2025, Thérèse et Isabelle de Marie Fortuit est une adaptation du texte éponyme de Violette Leduc censuré en 1954 pour « obscénité énorme et précise ». Dans cet entretien réalisé à Paris le 3 juillet 2025, elle revient sur ses choix d’adaptation et commente ses documents de travail.

ABSTRACTCopper Radical Alcohol Oxidases (CRO-AlcOx), which have been recently discovered among fungal phytopathogens are attractive for the production of fragrant fatty aldehydes. To investigate the secretion of CRO-AlcOx by natural fungal strains, we undertook time-course analyses of the secretomes of threeColletotrichumspecies (C. graminicola, C. tabacumandC. destructivum) using proteomics. The addition of a copper-manganese-ethanol mixture toColletotrichumcultures unexpectedly induced the secretion of up to 400 proteins, 29-52% of which were carbohydrate-active enzymes (CAZymes), including a wide diversity of copper-containing oxidoreductases from the auxiliary activities (AA) class (AA1, AA3, AA5, AA7, AA9, AA11-AA13, AA16). Under these specific conditions, while a CRO-glyoxal oxidase from the AA5_1 subfamily was among the most abundantly secreted proteins, the targeted AA5_2 CRO-AlcOx were secreted at lower levels, suggesting heterologous expression as a more promising strategy for CRO-AlcOx production and utilization.C. tabacumandC. destructivumCRO-AlcOx were expressed inPichia pastorisand their preference toward both aromatic and aliphatic primary alcohols was assessed. The CRO-AlcOx fromC. destructivumwas further investigated in applied settings, revealing a full conversion of C6 and C8 alcohols into their corresponding fragrant aldehydes.IMPORTANCEIn the context of the industrial shift toward greener processes, the biocatalytic production of aldehydes is of utmost interest owing to their importance as intermediates in preparative chemistry and for their use as flavors and fragrances ingredients. In the search for new biocatalysts, CRO-AlcOx have the potential to become platform enzymes for the oxidation of alcohols to aldehydes. The use of crude fungal secretomes is often seen has an appealing approach by industries since alleviating various costs pertaining to biocatalysts production. However, the secretion of CRO-AlcOx by natural fungal strains has never been explored. This study showed thatColletotrichumspecies can secrete a broad diversity of copper-containing enzymes, but only little amount of CRO-AlcOx. Thus, recombinant expression remains the most promising approach. The potential of CRO-AlcOx as biocatalyst for flavor and fragrance applications was confirmed through the production of two new enzymes with activity on fatty alcohols.

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Il semble impossible de dire du mal d’un roman animé d’une telle générosité et d’un tel amour pour l’amour. Les quelques critiques lues ici et là éclatent d’ailleurs en comparaisons élogieuses et exotiques, comme celles de la rubrique du Temps (22 août 2015) : ce roman « se boit comme un jus de mangue » ou en adjectifs démesurés : « langue superbe », « rutilance », « bain chaud de couleurs et d’émotion », tant il est incontestable que Douna Loup a une écriture propre, sensuelle et invasive, q...

Abstract Series of differently substituted tricyclic ethers have been synthesized on a multi‐gramme scale from a common cis‐1,2‐dimethyl norborane structure. Olfactory evaluation of these compounds is presented and reveals mainly woody aspects as the predominant notes. Copyright © 2014 John Wiley & Sons, Ltd.