Nederlandse Vereniging voor Kindergeneeskunde

Elizabeth Casson Trust Research Grant_2022 (UK); Health Research Board (Ireland) & HSC Public Health Agency (Grant ESI-2021-001) through Evidence Synthesis/Cochrane Ireland REGISTRATION: Protocol DOI 10.1002/14651858.CD015371.

Third party use of charging infrastructure for electric buses on major passenger transport hubs offers potential to make better use of existing electricity grid capacity, especially given the current lack of grid capacity. This requires flexibility in the charging operation, which is hampered due to lacking ownership of this opportunity to share charging infrastructure. This research aims to develop understanding of the perception of drivers and barriers for stakeholder organizations (SOs) to initiate collaboration on flexible use of this infrastructure. A survey under 141 respondents was applied to collect these perceptions on the drivers and barriers, the most logical initiator and motives behind these choices. Results indicate that presence of sufficient benefits, fitting organizational culture and entrepreneurial freedom are main drivers for initiation of collaboration. No specific barriers stood out. Furthermore, public parties – specifically the transport authority – are indicated as most logical initiator. The paper shows that challenge for initiating collaboration lies in the unclarity around the mandate to take initiative. Although the presence of mandate is not seen as main driver for one’s own organization, it is seen as reason why other organizations should be the initiator. The lacking overall mandate for collaboration causes inertia, and thereby is the reason why the fire is not lit yet. Fortunately, this challenge of ownerless opportunities in complex SO-environments can be overcome, but it does require a new way of looking at how infrastructure is applied and managed.

The defined vascularization of complex and intricate tissue constructs remains an unmet need in tissue engineering and regenerative medicine. While large constructs require vasculature for oxygen, nutrient supply, and waste clearance, their incorporation within biofabricated tissues is essential for developmental and disease modeling studies. There is, therefore, a critical demand to establish reproducible and organized vascular networks within in vitro models to ensure experimental robustness and quantitative interpretability. Current micropatterning and biofabrication strategies are limited in emulating native geometrical complexity, throughput, and resolution, while self-assembly approaches rely on inherently random network formation. Here, laser-induced forward transfer (LIFT) is utilized, offering high spatial resolution for deterministic micropatterning of cells with high viability. A unique droplet quality assessment framework is established through a multiparametric study to objectively identify a printability window, assigning a single-indexed score per printing condition. Within the optimal transfer regime, control over droplet concentration is demonstrated. The impact of pattern density on early vascular morphogenesis is explored, highlighting the effect of geometrical design on network formation. Finally, these findings are leveraged for the spatially controlled assembly of multicellular vascular patterns, offering a reproducible strategy for high-resolution micropatterning and addressing a key limitation in the biofabrication of physiologically relevant tissue models.

To study large-scale convective flows (fluid motion in a thin layer), it is possible, for initial studies, to consider the Stokes approximation when integrating the Oberbeck–Boussinesq equation. The convective derivative in the momentum transfer equations and in the heat conduction equation is in this case assumed to be identically equal to zero. The paper discusses several approaches to constructing exact solutions for slow (creeping) flows of a non-uniformly heated fluid. Formulas for three-dimensional flows in the Lin–Sidorov–Aristov class are given for steady flows. The hydrodynamic fields are described by polynomials. Exact solutions are given for the velocity field nonlinearly depending on two spatial coordinates (longitudinal, or horizontal) with coefficients of nonlinear forms, which depend on the third coordinate. The study shows how it is possible to automate the computation of unknown coefficients for the formation of hydrodynamic fields (velocities and temperatures).

З формуванням в Україні ринкових умов лікування тварин та надання ветеринарно-санітарних послуг все більше стають прерогативою приватної ветеринарної медицини. Незважаючи на умови жорсткої конкуренції, в якій працюють комерційні заклади та лікарі-підприємці, ринок ветеринарних послуг весь час зростає. Проте ця сфера залишається менш дослідженою і поширеною порівняно з іншими галузями. Отже, започаткування бізнесу у сфері надання ветеринарних послуг, навіть при наявності конкуренції, є перспективним напрямом підприємницької діяльності, це зумовлює актуальність проведеного дослідження. Метою роботи є здійснення аналізу тенденцій розвитку ветеринарних послуг в різних регіонах України та визначення областей, які можуть бути потенційно привабливими для започаткування ветеринарного бізнесу. Для цього проведено аналіз кількості закладів ветеринарної медицини у регіонах України, виділено, п’ять регіонів, де спостерігається стрімкий розвиток сфери ветеринарних послуг. Ці регіони розглядаються як найбільш перспективні для підприємництва. Крім того, проведено прогноз розвитку ветеринарного бізнесу в трьох регіонах за допомогою різних методів короткострокового прогнозування, зокрема із застосуванням моделей Хольта – Вінтерса, Брауна та методу ковзного середнього. Виявлено найбільш застосовні підходи для прогнозування на два роки. Отримані оцінки прогнозу підтвердили його адекватність. Проведений аналіз підтвердив потенціал започаткування бізнесу у сфері надання ветеринарних послуг і дозволив визначити найбільш перспективні регіони для започаткування ветеринарного бізнесу. Майбутні наукові дослідження можуть бути спрямовані на моделювання розвитку ветеринарних послуг з урахуванням специфіки обраних регіонів та напрямків ветеринарної медицини. Поглиблене вивчення динаміки цієї галузі дозволить виявити та використати нові можливості для розвитку бізнесу в сфері ветеринарної медицини в Україні.

This article deals with the role and effective use of innovative technologies in teaching English, and also provides links to Internet resources that are effectively used in the classroom

Protein lysine lactylation (Kla) has been proved to be closely related to inflammatory diseases, but its role in periodontitis (PD) is unclear. Therefore, this study aimed to establish the global profiling of Kla in PD models in rats. Clinical periodontal samples were collected, the inflammatory state of tissues was verified by H&E staining, and lactate content was detected by a lactic acid kit. Kla levels were detected by immunohistochemistry (IHC) and Western blot. Subsequently, the rat model of PD was developed and its reliability verified by micro-CT and H&E staining. Mass spectrometry analysis was conducted to explore the expression profile of proteins and Kla in periodontal tissues. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed, and a protein-protein interaction (PPI) network was constructed. The lactylation in RAW264.7 cells was confirmed by IHC, immunofluorescence and Western blot. The relative expression levels of inflammatory factors IL-1β, IL-6, TNF-α, macrophage polarization-related factors CD86, iNOS, Arg1, and CD206 in RAW264.7 cells were detected by real time-quantitative polymerase chain reaction (RT-qPCR). We observed substantial inflammatory cell infiltration in the PD tissues, and the lactate content and lactylation levels were significantly increased. The expression profiles of protein and Kla were obtained by mass spectrometry based on the established rat model of PD. Kla was confirmed in vitro and in vivo. After inhibiting the "writer" of lactylation P300 in RAW264.7 cells, the lactylation levels decreased, and the expression of inflammatory factors IL-1β, IL-6, and TNF-α increased. Meanwhile, the levels of CD86 and iNOS increased, and Arg1 and CD206 decreased. Kla may play an important role in PD, regulating the release of inflammatory factors and polarization of macrophages.