Public Risk Management Association

Cooked meat products, particularly ham, are widely consumed, and reducing nitrite levels has become a priority due to health concerns and regulatory pressure. This study evaluated the microbiological safety, technological performance, physicochemical properties, and sensory attributes of whole cooked ham formulated with reduced nitrite (from 150 to 80ppm) during shelf life. Microbiological analyses were conducted every 15days, including total viable counts (TVC), lactic acid bacteria (LAB), Enterobacteriaceae, Staphylococcus aureus and Escherichia coli. TVC and LAB remained below the safety threshold (<104CFU/g), while all other parameters were below detection limits. Sulphite reducing Clostridium spores, Salmonella spp. and Listeria monocytogenes were absent from all samples. Challenge testing with L. monocytogenes and Clostridium sporogenes was performed to assess the product's ability to inhibit pathogen growth under simulated storage conditions (up 35 and 90days, respectively) and temperature abuse conditions (8°C). The reduced-nitrite ham formulation effectively inhibited the growth of C. sporogenes and delayed the growth of L. monocytogenes. Technological assessments included colour measurements, water retention capacity, and texture profile analysis (TPA), with no significant differences observed between the standard and nitrite reduced formulations (P>0.05). Physicochemical parameters such as pH (6.0-6.2), water activity (aw, 0.9669-0.9482), and residual nitrite content (4 to 1mg/kg) were evaluated at 0, 45 and 90days. These findings demonstrate that reducing nitrite levels to 80ppm can ensure the product safety and quality, as evidenced by stable physicochemical properties and the preservation of sensory characteristics such as appearance, odour, texture, and flavour.

O objetivo deste estudo é analisar os efeitos da pandemia de Covid‑19 (2020–2022) sobre a saúde mental de profissionais de enfermagem. Trata‑se de uma revisão integrativa da literatura, baseada em artigos científicos indexados na base Literatura Latino‑Americana e do Caribe em Ciências da Saúde (LILACS), acessada por meio da Biblioteca Virtual em Saúde. Foram incluídos estudos publicados em português, entre 2020 e 2022, que abordaram a saúde mental de profissionais de enfermagem no contexto da pandemia de Covid‑19. A amostra final totalizou cinco artigos, que compuseram o corpus da revisão. Os resultados evidenciam alta frequência de distúrbios psíquicos menores e transtornos mentais comuns, piora da qualidade de vida, sobrecarga de trabalho, medo de contágio, precariedade das condições de trabalho e insuficiente apoio institucional. Tais fatores repercutiram diretamente na saúde mental da equipe de enfermagem durante o período analisado. Conclui‑se que a pandemia expôs e intensificou fragilidades estruturais já existentes na organização do trabalho em enfermagem, contribuindo para o sofrimento psíquico desses profissionais. Embora os estudos incluídos se refiram à fase inicial e mais crítica da Covid‑19, seus achados permanecem relevantes no cenário pós‑pandêmico, subsidiando o planejamento de estratégias de promoção, prevenção e cuidado em saúde mental voltadas aos profissionais de enfermagem.

Objetivo: Descrever as percepções e expectativas de gestantes sobre as práticas da Atenção Primária à Saúde no pré-natal. Método: Estudo descritivo, qualitativo, realizado em Unidades Básicas de Saúde de um município do Pernambuco, com 12 gestantes acompanhadas pela Estratégia Saúde da Família. A coleta ocorreu por meio de entrevistas semiestruturadas e a análise seguiu a técnica de análise de conteúdo de Bardin. Resultados: As gestantes eram majoritariamente adultas jovens, com baixa renda e inserção laboral fora do domicílio. Destacou-se o papel da enfermagem na escuta e orientação, e do Agente Comunitário de Saúde na adesão ao cuidado, além de fragilidades estruturais. Conclusão: As gestantes valorizam acolhimento e escuta ativa, reforçando a necessidade de cuidado humanizado.

Vocational education and training are essential for the integration of refugee youth into Greek society. Therefore, it seems that there is an urgent need for the Greek Ministry of Education and Religion and for the Ministry of Labour (herewith, Greek Ministries of Education and Labour) to redesign and adapt their strategies and practices to address the specific learning needs of refugee youth. The aim of this study is to explore the types of policies that the Ministries of Education and Labour should design, reform, and implement to increase the number of students attending vocational education and training in Greece. The researchers adopted a qualitative approach, using semi-structured interviews and questionnaires. Ten participants took part in the study: seven Refugee Education Coordinators based in camps across Greece, two experts from the European Centre for the Development of Vocational Training (CEDEFOP), and one academic specializing in adult education and serving as an EPALE Ambassador. The findings indicate that both Ministries of Education and Labour need to collaborate more closely in redesigning and implementing targeted policies for refugee students. These should include expanding reception classes in EPAS (vocational school) and EPAL (vocational high school) schools, reducing bureaucratic procedures, ensuring adequate staffing with trained teachers, and adopting simpler administrative processes.

Technick univerzita Liberec, Fakulta prodovdn-humanitn a pedagogick, Katedra primrn a preprimrn pedagogikyTradin esk frazmy tvo vznamnou soust kulturnho vdom a odrej zpsoby, jimi rzn generace strukturovaly zkuenost a hodnoty.Akoli dnes v jazyce mladch generac asto ustupuj, jejich interpretace zstv dleitm prostedkem rozvoje jazykovho i kognitivnho vdom

Inclusion is an educational approach that values diversity and ensures equal rights and opportunities for all individuals, regardless of their background or abilities. The continuous professional development of educators plays a pivotal role in advancing this approach, as it enables them to strengthen their competencies, acquire new knowledge, adapt to contemporary pedagogical practices, and implement effective inclusion strategies in the classroom. The present study examines the willingness of primary education teachers in the Attica region of Greece to engage in professional training, with a particular focus on gender differences. It also explores variations in knowledge of the legal framework supporting inclusive education between special education and general education teachers. The study involved 304 educators, and data was collected through a structured questionnaire designed in accordance with current research literature. The sample was predominantly female (89.1%), reflecting the demographic composition of primary education teachers in the region. The findings revealed that general education teachers demonstrated a moderate level of knowledge regarding the legal framework supporting inclusive education, whereas special education teachers exhibited a higher level of understanding. Moreover, male educators appeared more willing to learn from other professionals to enhance their inclusive practice, while female educators showed a greater tendency to critically reflect on their beliefs about students with special educational needs.

Cílem článku je popsat současnou situaci v přípravných třídách a informovat o návrzích k její proměně. Každoročně je do přípravných tříd zařazován vysoký počet dětí. Skupina poslanců připravila v roce 2024 návrh pro snížení počtu dětí s odkladem školní docházky zaměřený na práci učitelů v předškolní třídě, na práci pedagogicko-psychologických poraden i na práci učitelů první třídy.

Il presente contributo propone alcune attività scolastiche di educazione alla pluralità basate sull’utilizzo dei canti di tradizione orale nello studio della storia. L’articolo ne mette in luce l’utilizzo quali preziose fonti per la lettura dei fatti storici da diverse prospettive, con particolare attenzione al punto di vista delle classi subalterne, spesso escluso dalle narrazioni canoniche.

Abstract This study aims to explore the impact of lean-green practices on sustainable supply chain performance, with a focus on the moderating effects of top management engagement, organizational change management, collaborative synergy, and policy initiatives. The study's population consisted of 2,194 songket artisan groups, as documented by the Bali Provincial Trade and Industry Office. Utilizing the Slovin formula, a sample of 584 artisan groups was selected from nine regencies and one municipality in Bali Province. Proportionate Stratified Random Sampling was employed to choose the sample units, ensuring proportionality in the selection process. Data collection was conducted through a questionnaire utilizing a 1–5 Likert scale. Data analysis was performed using Structural Equation Modeling (SEM) with Partial Least Squares (PLS) analysis. The findings suggest that lean practices have a positive influence on green practices, which in turn significantly enhance sustainable supply chain performance. Green practices serve as a mediator in the relationship between lean practices and sustainability outcomes. Furthermore, organizational change management and collaborative synergy amplify these positive effects, while top management engagement and policy initiatives do not exhibit significant moderating roles. These results underscore the importance of integrating lean and green strategies, supported by effective organizational and collaborative mechanisms, to enhance supply chain sustainability in SMEs, providing valuable insights for both theoretical and practical applications in sustainable operations management.

Targeted insertion (TIN) of transgenic trait cassettes has the potential to reduce timeline and cost for GM product development and commercialization. Precise genome engineering has made remarkable progress over the last several decades, particularly with the use of site-directed nucleases as core editing machinery. However, there are still many critical factors that can impact TIN efficiency including insertion site selection, nuclease optimization and expression, donor vector design, gene delivery, and stable event regeneration. Here, we develop workflows for target site sequence identification and gRNA screening for CRISPR-Cas12a system and demonstrate its successful application for TIN in maize with donor sequences up to 10 kilobase pairs (kb) in size. We first prioritize genomic regions for inserting transgenes in silico using bioinformatics tools and then test gRNA performance using a leaf protoplast transient assay. Despite its known low efficiency, we identify homology-directed repair (HDR) as the preferential pathway for directing targeted insertions of large sequences in immature embryos and demonstrate double-junction integrations at a rate of up to 4%. We further apply a molecular analysis workflow using large amplicon TaqMan assays and nanopore sequencing for streamlined identification and characterization of high-quality insertion events with intact large inserts. Analysis of TIN events across generations suggests that efficiency bottlenecks are not limited to donor targeted integration; attrition in efficiency also results from partial or additional donor insertion, chimerism, and close linkage with undesired sequence insertions such as those encoding the editing machinery. This work represents a major step forward in realizing the potential of precise genome engineering in maize for basic research and biotech trait development applications.