Temple University Ambler

<strong class="journal-contentHeaderColor">Abstract.</strong> Accurate assessment of leaf functional traits is crucial for a diverse range of applications from crop phenotyping to parameterizing global climate models. Leaf reflectance spectroscopy offers a promising avenue to advance ecological and of robust hyperspectral models for predicting leaf photosynthetic capacity and associated traits from reflectance data has been hindered by limited data availability across species and environments. Here we introduce the Global Spectra-Trait Initiative (GSTI), a collaborative repository of paired leaf hyperspectral and gas exchange measurements from diverse ecosystems. The GSTI repository currently encompasses over 7500 observations from 397 species and 41 sites gathered from 36 published and unpublished studies, thereby offering a key resource for developing and validating hyperspectral models of leaf photosynthetic agricultural research by complementing traditional, time-consuming gas exchange measurements. However, the development capacity. The GSTI database is developed on GitHub (<a href="https://github.com/plantphys/gsti" target="_blank" rel="noopener">https://github.com/plantphys/gsti</a>) and published to ESS-dive <a href="https://data.ess-dive.lbl.gov/datasets/doi:10.15485/2530733" target="_blank" rel="noopener">https://data.ess-dive.lbl.gov/datasets/doi:10.15485/2530733</a>, Lamour et al., 2025). It includes gas exchange data, derived photosynthetic parameters, and key leaf traits often associated with traditional gas exchange measurements such as leaf mass per area and leaf elemental composition. By providing a standardized repository for data sharing and analysis, we present a critical step towards creating hyperspectral models for predicting photosynthetic traits and associated leaf traits for terrestrial plants.

Stormwater control measures (SCMs) such as retention basins, bioswales, and bioinfiltration systems are used to reduce peak flows and remove pollutants from stormwater in temperate urban landscapes. However, the application of de-icing salts to roadways can substantially increase the salinity of stormwater basin media (i.e., engineered soil), likely impacting the physical properties of these soils. Further, SCM soils can become moderately compacted, potentially altering the extent and effects of salinization on soil physical properties. Although many studies have documented the high salinity of roadside soils in winter, the effects of salinity on soil hydraulic properties is not well understood, especially in the context of urban stormwater basins. Here, we compared the water retention properties (spanning pressure potentials of -10 to -1,000,000 hPa) of salinity-affected stormwater media (1-100 dS m-1, using Na+ and Mg2+ salts) that was either uncompacted or compacted. The effects of salinity on both matric and osmotic potential included shifts in the plant-available range with the magnitude depending on a combination of salt type and concentration. We attribute these changes to salinity inducing shifts in both surface tension and pore size distributions. Further, compaction increased the severity of salinization under low salinity conditions but not high. Climate change may increase the number and intensity of snow events in many temperate urban regions, which may increase salt loads to stormwater control measures, exacerbating the aforementioned effects.

Introduction: Few therapies have produced significant improvement in cardiac function after ischemic cardiac injury (ICI). The activation of local inflammatory responses is critical to cardiac repair after ICI. Our previous studies showed that cortical bone derived stem cells (CBSCs) possess can enhance repair after ICI. Beneficial effects of CBSCs appear to be mediated by paracrine factors including extracellular vesicles (EVs). This study explored if and how these EVs enhance cardiac repair by modulating the ICI immune response. Hypothesis: CBSCs derived extracellular vesicles (CBSC-EV) modulate immune response after ICI. Methods and Results: Both CBSCs and CBSC-EV were transplanted into mice after myocardial infarction (MI). CBSCs and CBSC-EV treated animals had better ICI repair compared with Saline, with reduced scar size, attenuated structural remodeling, improved cardiac function, and reduced cell apoptosis. These effects were linked to alteration of immune response. Plasma analysis of CBSCs and CBSC-EV treated animals showed significantly lower level of pro-inflammatory cytokines such as TNFα 24 hours after MI. CBSCs and CBSC-EV treatment induced significant polarization from CD86+ M1 macrophages towards CD206+ M2 macrophages phenotype 5 days post-MI, with subsequent reduction of CD8+ T cells and increase of CD4+ T cells, especially the FoxP3+ Treg population, from 7 days to 14 days post-MI. RNA sequencing analysis revealed that CBSC-EV contained a distinct transcriptome compared with endothelial progenitor cells and cardiosphere-derived cells. Gene Ontology analysis suggested the differentially expressed genes in CBSC-EV significantly enriched in immune cell receptor binding. MiR-182 and miR-183, which ranked top of the most significantly upregulated genes in CBSC-EV, attenuated M1 macrophage polarization after LPS treatment and promote CD25+ FoxP3+ Treg differentiation in vitro. Conclusions: CBSC-EV enhance cardiac repair by modulating the immune response after injury and highlight the molecular bases of into the beneficial effects of cell-free therapy after ICI.

Climate models predict that, in the coming decades, many arid regions will experience increasingly hot conditions and will be affected more frequently by drought. These regions are also experiencing rapid vegetation change, notably invasion by exotic grasses. Invasive grasses spread rapidly into native desert ecosystems due, in particular, to interannual variability in precipitation and periodic fires. The resultant destruction of non-fire-adapted native shrub and grass communities and of the inherent soil resource heterogeneity can yield invader-dominated grasslands. Moreover, recurrent droughts are expected to cause widespread physiological stress and mortality of both invasive and native plants, as well as the loss of soil resources. However, the magnitude of these effects may differ between invasive and native grasses, especially under warmer conditions, rendering the trajectory of vegetated communities uncertain. Using the Biosphere 2 facility in the Sonoran Desert, we evaluated the viability of these hypothesized relationships by simulating combinations of drought and elevated temperature (+5°C) and assessing the ecophysiological and mortality responses of both a dominant invasive grass (Pennisetum ciliare or buffelgrass) and a dominant native grass (Heteropogan contortus or tanglehead). While both grasses survived protracted drought at ambient temperatures by inducing dormancy, drought under warmed conditions exceeded the tolerance limits of the native species, resulting in greater and more rapid mortality than exhibited by the invasive. Thus, two major drivers of global environmental change, biological invasion and climate change, can be expected to synergistically accelerate ecosystem degradation unless large-scale interventions are enacted.

Como a tecnologia da informação amplia a assimetria de poder entre os Estados contemporâneos? Com o objetivo de responder ao questionamento, o artigo descreve o processo de utilização do ciberespaço para consecução dos objetivos estratégicos da Federação Russa em seu entorno regional, durante o conflito desencadeado com a Ucrânia (2014-2015). A partir da análise de relatórios de empresas especializadas em segurança cibernética e instituições governamentais, aplicamos as técnicas qualitativas da dependência da trajetória e rastreamento de processos para explicitar a complexidade das operações conjuntas entre as forças especiais russas e hackers civis, bem como a sofisticação das principais ameaças e armas utilizadas nos ataques cibernéticos. Desse modo, identificamos o mecanismo responsável por conectar os níveis estratégico, tático e operacional militar ao verificarmos o processo de ação simbiótica entre os atores envolvidos no conflito. As evidências coletadas indicam como a guerra híbrida empregada pela Federação Russa incorporou a dimensão cibernética como peça chave para a desestabilização de territórios e consecução de interesses em seu entorno estratégico.

Spanish-speaking families in the United States must often overcome multiple challenges to support their young children’s early language development (e.g., language and cultural barriers, financial stress, limited learning resources, etc.). These challenges highlight the need for early language interventions tailored to the needs of Spanish-speaking families and developed in collaboration with them. For diverse populations, early language interventions which are both translated into the relevant languageandculturally responsive are the most effective for improving child outcomes. However, few interventions meet both criteria, demonstrating a need for materials that are accessible across both language and culture. The current study describes the five-phase process of creating a linguistically and culturally relevant Spanish adaptation of Duet, an early language intervention. The adaptation of the Duet intervention modules involved multiple language experts, including Spanish-speaking developmental psychologists, a translation company, and Spanish-speaking caregivers of infants and toddlers. Fourteen caregivers were recruited to participate in two, 3-h focus groups. Input from caregivers was a particularly important step in the adaptation process, as caregivers hold knowledge about everyday experiences with their children. Through this process, the authors aim to shed light onto the importance of collaborating with the community and present a possible framework for others who are adapting interventions.