Tianjin Conservatory of Music

Radiation enteritis is a common complication in patients undergoing abdominal radiotherapy. Current management strategies face significant limitations: clinical agents like amifostine are hindered by systemic side effects and demanding administration; direct supplementation with radioprotective metabolites such as propionate suffers from low bioavailability and transient action; and conventional probiotics lack targeted therapeutic output. To address these challenges, we engineered Escherichia coli Nissle 1917 to function as a living therapeutic that continuously produces and delivers propionate directly in the gut. This propionate-engineered probiotic achieved a production yield of 181.33 ± 4.27mg/L in vitro. In a mouse model of abdominal irradiation, this engineered bacterium alleviated radiation-induced intestinal damage by continuously releasing propionate and enhancing intestinal epithelial barrier function. Multi-omics analysis revealed that the engineered bacterium could restore intestinal microbiota homeostasis, enhancing the abundance of advantageous bacteria with radioprotective properties (e.g., Dubosiella, Akkermansia). Moreover, it modulated intestinal microbiota metabolism, influencing the metabolism of ascorbic acid, aldoses, and other metabolites. Additionally, it protected the intestinal mucosal barrier from radiation-induced damage, which was associated with the modulation of the SOCS1/JAK2/STAT3 signaling pathway. This study introduces a novel biological therapy to mitigate the side effects of radiotherapy and could open new avenues for preventing and treating radiation-induced intestinal injury.

ABSTRACT Interfacial cracking is the main reason for the premature spallation of thermal barrier coatings (TBCs), which is directly related to the thermally grown oxide (TGO) growth and top coat (TC) sintering and creep. To elucidate the interfacial cracking behaviors, the interface cracking model of TBCs was developed. The effects of TGO growth, TC sintering, and creep on the interfacial crack evolution were studied. Results showed that significant increases in the tensile stress (13.4%) and shear stress (18.4%) at the interface were caused by sintering. The high tensile stress led the crack to nucleate in the valley region and spread along the TC/TGO interface towards the peak region. Sintering resulted in a 28.6% reduction in the time required for crack initiation. Furthermore, a large TC creep rate relaxed the interfacial stress significantly so as to postpone the time to reach the critical stress, while the high TGO growth rate accelerated interfacial cracking. Crack occurred after 90, 25, and 10 thermal cycles and propagated for 0.0028, 0.0345, and 0.0399 mm after 100 thermal cycles under the three different TGO growth rates, respectively. In addition, the TC creep rate and the TGO growth rate influenced the transition location of the interface fracture type (I and II).

Acute leukemias of ambiguous lineage (ALAL) is a rare type of hematologic malignancies with poor outcomes. Currently, the treatment for this type of leukemia lack standardized protocols and exhibit significant heterogeneity, necessitating the exploration of novel, targeted approaches. We retrospectively analyzed the clinical characteristics, efficacy, and safety of 13 newly diagnosed Philadelphia chromosome (Ph)-negative ALAL patients between May 2022 and March 2025, who received induction therapy with the mini-CVD regimen (cyclophosphamide, vincristine, dexamethasone) combined with venetoclax and azacitidine. The cohort comprised 8 males and 5 females, with a median age of 53 years (range, 28–73 years). Among this cohort, 92.3% of patients (12/13) achieved complete remission (CR). Three patients underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT) during CR1, and one patient received salvage allo-HSCT following relapse. With a median follow-up of 9.0 months (range 1.2–35.4 months), the overall survival rate was 84.6% (11/13). One patient died due to primary resistance with sustained disease progression, and another succumbed to post-transplantation complications. Of the 12 patients who achieved CR, 2 (15.4%) experienced relapsed, but both attained a second CR after salvage therapy. During induction therapy, the overall incidence of infection was 76.9% (10/13), with the majority being pulmonary infections (5/10). Notably, there was no death during induction therapy. Our data demonstrated that this regimen showed a high CR rate, with manageable toxicity, offering a promising therapeutic approach for this rare and challenging leukemia subtype.

With the advancement of China's "dual carbon" goals, ESG ratings have become a core reference for green credit decision-making. However, high-carbon enterprises may engage in "greenwashing" by strategically disclosing information to embellish their environmental performance for financing purposes, creating a "brown-greenwashing" paradox that challenges the effectiveness of transition finance. To examine this issue, this study innovatively constructs a "greenwashing index" based on data from high-carbon industries such as coal power and steel in China's A-share market from 2015 to 2023. It employs a benchmark regression model for empirical analysis. The study found that ESG ratings have a significant positive impact on the scale of green credit. Still, this effect is significantly negatively moderated by carbon emission intensity, confirming the "greenwashing" behavior of high-emission enterprises. Further analysis reveals that, compared to private enterprises, state-owned enterprises are more likely to obtain green credit through ESG ratings, highlighting the resource bias associated with ownership. This study provides empirical evidence for identifying and curbing "brown washing" and offers targeted recommendations for improving ESG ratings and optimizing credit policies.

Platycodonis Radix is a well-known traditional Chinese medicine (TCM) for the treatment of respiratory disease. However, its mechanism underlying the traditional efficacy remains unclear and warrants further investigation. The present study aims to elucidate the effective substance and molecular mechanisms. HPLC-QTOF-MS/MS and GC-MS were used to identify the chemical compositions; network pharmacology was adopted to predict the effective substance and possible mechanism. Meanwhile, an invitro experiment was employed to validate the pharmacological mechanisms. In total, 51 nonvolatile and 20 volatile compounds were tentatively characterized in this study. Among them, 16 active ingredients can act on 323 action targets and 162 signaling pathways to relax bronchial smooth muscle and inhibit airway inflammation and remodeling. Especially, four main predicted active compounds, platycodin D (PD), PE, PD3, and deapio platycodin D (DPD), exerted anti-inflammatory effects by reducing the secretion of NO, PGE2, IL-6, and IL-17 in LPS-induced RAW 264.7 cells. Furthermore, platycodin D could inhibit the protein expression of HSP90, TRAF6, phospho-NF-κB p65, and phospho-ERK1/2. This study analyzed the chemical composition of Platycodonis Radix, clarified that the main active ingredients were triterpenoid saponins, and confirmed the IL-17 signaling pathway as its anti-inflammatory mechanism, which laid the foundation for more in-depth mechanistic research and clinical application.

Safeguarding intangible heritage music increasingly depends on endtoend digital value chains that can faithfully model, generate, track, and remunerate culturally embedded musical knowledge. This paper proposes a generative AI framework that jointly (i) learns a multilayer, ethnomusicologyaware representation across raw audio, symbolic structure, and contextual metadata, (ii) constrains a controllable transformerdiffusion pipeline to preserve modality, microtonal tuning, ornamentation practice, and rhythmic grammar, and (iii) embeds blockchainanchored provenance and smartcontract royalty logic inside the creative pipeline. Using 1,842 recordings (126.3 hours) spanning Southeast Asian gamelan, Chinese Buddhist chant, and Andean panpipe repertoires, we compare our system against an archiveonly baseline and an unconditioned generative baseline. Conditioned generation reduces average modal deviation from canonical tunings from 12.34.1 cents to 4.91.8 cents, decreases rhythmic dynamictimewarping distance by 44.5%, and raises expert authenticity ratings from 3.120.61 to 4.470.28 (ICC(2,k)=0.82). On the valuechain layer, median royalty settlement time drops from 23.7 days to 1.92 days, the Theil inequality index falls from 0.218 to 0.071, and Jains fairness index rises from 0.63 to 0.89. Listenerside evaluation shows higher longtail coverage (+31.4%), improved NDCG@20 (0.3820.497), and a 26% reduction in the hazard of early session abandonment. The findings demonstrate that culturally bounded, rightssensitive generative pipelines can simultaneously enhance preservation fidelity, creative reuse, and equitable community remuneration.

BackgroundTerminalia chebula Retz, known as the King of Tibet, is considered a functional food in China, celebrated for its antioxidant, immune-modulating, antibacterial, and anti-inflammatory properties. Chebulinic acid, derived from aqueous extracts of Terminalia chebula Retz, is known for its anti-inflammatory properties. However, its potential as an anti-Helicobacter pylori (HP) agent has not been fully explored.MethodsHerein, we extracted the main compound from Terminalia chebula Retz using a semi-preparative liquid chromatography (LC) system and identified compound 5 as chebulinic acid through Ultra-high performance liquid chromatography-MS/MS (UPLC–MS/MS) and Nuclear Magnetic Resonance (NMR). To evaluate its role, we conducted minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays, scanning electron microscope (SEM) imaging, inhibiting kinetics curves, urea fast test, cell counting kit-8 (CCK-8) assay, western blot analysis, griess reagent system, and molecular docking.ResultsOur results showed that chebulinic acid effectively inhibited the growth of the HP strain ATCC 700392, damaged the HP structure, and exhibited selective antimicrobial activity without affecting normal epithelial cells GES-1. Importantly, it suppressed the expression of Cytotoxin-associated gene A (Cag A) protein, a crucial factor in HP infection. Molecular docking analysis predicted a strong affinity (−9.7 kcal/mol) between chebulinic acid and Cag A protein.ConclusionOverall, our findings suggest that chebulinic acid acts as an anti-adhesive agent, disrupting the adhesion of HP to host cells, which is a critical step in HP infection. It also suppresses the Cag A protein. These results highlight the potential of chebulinic acid against HP infections.

This paper explores the legal controversies and regulatory issues of forced technology transfer (FTT) in international trade, analyzing its stipulations under international law, the balance between intellectual property protection and technology transfer obligations, interpretations of relevant provisions in WTO and other international agreements, and the differences and conflicts in China's legal definitions of technology transfer. Through empirical analyses of WTO dispute cases such as DS603, DS611, and DS615, the study reveals the complexity and diversity of technology transfer in international trade, and proposes specific measures and recommendations for building a fair legal framework for technology transfer. These measures aim to strengthen intellectual property protection while promoting the free flow and development of technology, providing a balanced legal solution for technology circulation in international trade.