Universidad de Morón

Background: Fetuin (Ft) is the most abundant protein in fetal bovine serum (FBS) and is considered one of its essential components. This acidic glycoprotein plays a key role in cell adhesion and proliferation and is vital for maintaining in vitro cultures of animal and human cells, tissues, and organs. FBS is a natural source for Ft purification. However, the high demand for FBS as a standard reagent in cell culture severely limits its availability for use as a raw material for protein purification. Furthermore, the industrial production of FBS results in a significant amount of contaminated FBS. This contaminated fraction can thus be utilized for Ft recovery. Methods: In this work, we present a novel method for Ft recovery from FBS using a single chromatographic step based on anion exchange chromatography under acidic conditions. Results: Optimal adsorption conditions for Ft were studied using response surface methodology (RSM), which suggested a buffer pH of 4.2 and an FBS dilution of 40%. However, increasing the pH to 5 resulted in a 28% increase in Ft recovery, although with a slight reduction in Ft purity to 88%. A scale-up to half a liter of FBS was performed using a 400 mL column. A single-step elution with 0.3 M NaCl was employed, yielding an Ft recovery of 90% with a purity greater than 82%. Conclusions: The purified Ft demonstrated biological activity as a growth promoter in MDBK cell culture when utilized in a serum-free culture medium.

Mathematical modeling of virus dynamics is key to depicting the evolutionary pathways that lead to virus emergence, transmission, and persistence. Typically, viruses are populations of closely related genomes that continuously change their configuration and adapt to environmental selection pressures. In this work, we revisit this idea by considering viruses as active particles that dynamically shape their ecological niche. To this end, we adapt the Kinetic Theory of Active Particles to model virus interactions, allowing payoffs to co-evolve as a function of the populations configuration. We deduce the system of ordinary equations corresponding to the replicator dynamics with frequency-dependent payoffs. Then we obtain a nonlinear integro-differential equation describing the dynamics for a continuum of strategies by passing to the limit in the replicator system when the number of equations grows. Finally, we present some examples of virus dynamics.

<title>Abstract</title> Achieving room-temperature superconductivity in hydrides typically requires prohibitive pressures exceeding 150 GPa to stabilize the hydrogen clathrate structure. Here, we propose a non-equilibrium pathway to stabilize the superconducting phase of Yttrium-Hafnium Hydride (YHf₂H₂₄) at ambient pressure using a resonant acoustic drive. By targeting the hydrogen sublattice with a 45 GHz acoustic standing wave—derived from mass-scaling the acoustic modes of H₂₄ cages—we induce a parametric renormalization of the electron-phonon coupling constant (λ). Theoretical modeling based on the modified Allen-Dynes equation predicts a critical temperature T_c ≈ 298 K (25°C) with a drive amplitude of 7.5%, enabling operation at standard room temperature without any cooling requirements. Furthermore, we calculate the charge transport limits of this dynamically driven state. With a massive superconducting gap Δ ≈ 45 meV (f_gap ≈ 21.8 THz), a single nanoribbon interconnect supports a conservative data rate of 10.93 Tbps, outperforming PCIe 7.0 copper standards by a factor of &gt;80 while eliminating Joule heating. This 'Living Cable' architecture offers a scalable solution for exascale computing and consumer electronics without cryogenic cooling. To ensure robustness, we include detailed derivations, comparisons to recent ternary hydride advances, and discussions on potential experimental challenges, making this prediction consistent within current theoretical frameworks.

Modern nursing has evolved into a profession, and its contribution to healthcare is unquestionable. In Argentina, despite favourable changes, the nursing profession has yet to produce research knowledge that has an impact on health. In this document, the Asociación de Escuelas Universitarias de Enfermería de la República Argentina (AEUERA) provides recommendations for the development and implementation of policies to foster the development of health research by nursing in Argentina. The analysis of relevant evidence, academic discussion, and consensus among the participants of the AEUERA research commission led to the formulation of principles and recommendations. Five outlined principles underpin 20 recommendations. These principles represent the values that support a rigorous research practice, grounded in a robust scientific background and tailored to population needs. For Argentine nurses to investigate priority health problems, AEUERA recommends: 1) to guarantee access to scientific training programs with the same standards as other professions within the Argentine scientific system, 2) to guarantee access to doctoral scholarship programs, 3) to create job positions, protected by regulation, for nurse scientists, 3) to provide funding to conduct studies guided by both local and global health priorities. The implementation of these recommendations requires a sustained education and health policy to promote research training for this professional group. AEUERA urges decision-makers to consider these recommendations in their strategic planning for the sector.

Toxic cyanobacterial blooms, primarily caused by Microcystis aeruginosa, are increasing globally due to climate change. These harmful organisms have adapted to high temperature and UV radiation (UVR), posing a significant threat to drinking water. While UVBR can damage cyanobacteria, they may avoid it with antioxidant defense mechanisms. Microcystins (MCs), cyclic hepatotoxic heptapeptides produced by cyanobacteria, can harm aquatic and terrestrial organisms, but their role in antioxidant protection remains unclear. This study investigated the relationship between gene expression and MC content in Microcystis aeruginosa cells pre-adapted to elevated temperatures (29°C) exposed to natural UVR for several days. We have demonstrated a strong resilience in M. aeruginosa to high doses of UVBR, largely attributed to its enhanced antioxidant capacity following pre-exposure to elevated temperatures, as confirmed by previous studies. Moreover, our findings suggest that MCs may act as scavengers as part of the UVR protection mechanisms. This is evidenced by a temporal lack of correlation between the abundance of mcy transcripts and the cellular toxin content, where toxin quotas were lower despite increased mcy transcription. These findings are particularly relevant to the ecophysiological role of MCs, suggesting its potential involvement in increased blooms of toxic M. aeruginosa under conditions of climate change and eutrophication. This information is crucial for effective water treatment planning.

Introduction. ADHD is one of the most common neurodevelopmental disorders, affecting millions of children and adults worldwide. The significance of ADHD extends far beyond its prevalence. It impacts multiple areas of life, including academic achievement, job performance, relationships, and mental health. Significant challenges remain in terms of diagnosis and treatment. Methods. A search for information was conducted from January to June 2025 in the databases SciELO, Scopus, PubMed/MedLine, Google Scholar, Cochrane Library, as well as Clinical Keys services. From the resulting documents, those written in the last 10 years were selected; this time range was prioritized; some systematic reviews covered broader periods, such as the last 5 years, the last 10 years, or even did not apply a specific date limit. Conclusions. Attention-Deficit Hyperactivity Disorder (ADHD) represents a multifactorial challenge that transcends the clinical context to involve neurobiological, psychosocial, and cultural dimensions. Empowering patients and their families through psychoeducation strengthens understanding of the disorder, especially in vulnerable populations such as children, adolescents, and young adults. Investing in research, therapeutic innovation, and equitable public policies will be key to transforming ADHD care into a real opportunity to improve the quality of life for those who suffer from it.

This study aimed to evaluate the effect of gamma irradiation (0.6, 1.2, and 1.8 KGy doses) on rotavirus inactivation (RVA) and the quality of Esmerald fresh blueberries. Dosimetry measurements revealed absorbed doses of 0.72, 1.46, and 2.16 KGy. The D10 value for RVA inactivation was determined to be 3.03 ± 0.03 KGy, exceeding the maximum 2.5 KGy dose permitted for fresh fruit, indicating that gamma irradiation alone is insufficient for effective RVA inactivation without significantly impacting blueberry quality. On the other hand, the absorbed doses (0.72, 1.46, and 2.16 KGy) did not affect most of the physicochemical parameters or the nutritional profile. However, a dose-dependent decrease in mechanical properties and enzyme activity was observed. Therefore, achieving effective RVA inactivation in fresh blueberries could require a combined approach, integrating gamma irradiation, ideally at doses up to 1.46 KGy, with complementary methods. This combined strategy could offer a promising intervention for the inactivation of RVA in fresh produce. PRACTICAL APPLICATION: Blueberries, due to their specific characteristics, are particularly susceptible to viral contamination. Conventional sanitation methods, such as washing, are inadequate as they can affect the texture, integrity, and appearance of the fruit. Gamma irradiation is a non-thermal preservation method that improves food safety and extends shelf life while causing minimal alterations to the natural attributes of fresh produce. According to our results, gamma irradiation showed potential to inactivate RVA in blueberries. However, to achieve complete inactivation, a dose higher than the allowed dose (2.5 KGy) is required. Furthermore, our study revealed that irradiation treatments with doses lower than 2.5 KGy had a negative impact on the mechanical properties of blueberries, while they had a limited impact on the inactivation of the enzyme. In this regard, further studies are needed to explore combined strategies that effectively inactivate RVA and enzymes without compromising the delicate quality of fresh produce.