University of Isfahan

Cardiac MRI (CMRI) is crucial for assessing cardiovascular structure and function, yet manual analysis is time-consuming and subjective. This paper introduces CMRI Insight, an open-source, Python-based GUI tool designed for automated cardiac segmentation and motion tracking like CardioTrackNet in CMRI. Leveraging deep learning models like CardSegNet and MECardNet, it enables accurate delineation of the left ventricle, right ventricle, and myocardium with mean Dice scores of 95.8% for the left ventricle, 94.2% for the right ventricle, and 95.0% for the myocardium. The tool offers 3D mesh reconstruction, strain analysis, bull’s-eye visualization, and support for custom models. CMRI Insight enhances efficiency and reproducibility in cardiac imaging research and clinical evaluation, providing a user-friendly platform for comprehensive CMRI analysis.

In this study, it was attempted to answer the question: “ How does the concept of destination dupe expand or complicate current theories of destination substitutability?” using a case study approach. To this end, a professional research team traveled more than 700 km into the depths of the Lut Desert over a 5-year period, based on recorded GPS tracks. The team was accompanied by native guides and researchers as part of the “Lut Desert Mars Hike Tour Package,” in order to visit terrestrial analogs of Martian phenomena in the Lut Desert. The recently coined term “destination dupe,” as an extension of the concept of destination substitutability, refers to the tourists’ conscious fantasy, irrespective of the limitations associated with destination substitutability, where an example of the destination does not exist in the real world or lack a terrestrial equivalent. This gap has been addressed in the literature by focusing on distinctions between destination substitutability and destination dupe as extensively as possible.

Activating transcription factor 6 (ATF6) is a part of the unfolded protein response (UPR) system, which plays an important role in regulating endoplasmic reticulum stress. The overexpression of ATF6 has been reported in various malignancies; however, its expression and functional significance in B-cell precursor acute lymphoblastic leukemia (BCP-ALL) are poorly understood. Bone marrow samples from 69 pediatric patients withALL (48 newly diagnosed, 21 relapsed) and peripheral blood/bone marrow samples from 29 nonleukemic healthy children (controls) were collected. To investigate the effect of ATF6 inhibition on the viability of BCP-ALL derived cell lines, NALM-6 and SUP-B15 cells were treated with the ATF6 inhibitor Ceapin-A7 both alone and in combination with doxorubicin. Apoptosis was quantitatively assessed using flow cytometry. Theexpression levels of ATF6, BCL-2 family members, andATF6 target geneswere determined by qRT-PCR, and theprotein levels ofATF6, caspase-3, PARP, cell cycle regulatorsby Western blot analysis. Our results revealed that theATF6 expression was higher in pediatric patients with relapsed ALL than in newly diagnosed patients. Furthermore, inhibition of ATF6 with Ceapin-A7 enhanced doxorubicin-induced apoptosis in the BCP-ALL cell lines NALM-6 and SUP-B15. Upon inhibition of ATF6, both BCP-ALL cell lines were arrested at the G1 phase of the cell cycle. Combined treatment altered the expression of apoptosis-related and cell cycle regulatory genes and proteins, and ATF6 target genes were upregulated by Ceapin-A7 alone but attenuated in the combined treatmentgroup. ATF6 inhibition combined with doxorubicin represents a promising therapeuticstrategy for enhancing apoptosis in BCP-ALL cells.

[This retracts the article DOI: 10.1039/D2NA00394E.].