Venus Remedies (India)

This study aimed to assess the nephrotoxicity associated with VRP-034 (novel formulation of polymyxin B [PMB]) compared to marketed PMB in a three-dimensional (3D) kidney-on-a-chip model. To model the human kidney proximal tubule for analysis, tubular structures were established using 23 triple-channel chips seeded with RPTEC/hTERT1 cells. These cells were exposed to VRP-034 or PMB at seven concentrations (1-200 µM) over 12, 24, and 48 h. A suite of novel kidney injury biomarkers, cell health, and inflammatory markers were quantitatively assessed in the effluent. Additionally, caspase and cytochrome C levels were measured, and cell viability was evaluated using calcein AM and ethidium homodimer-1 (EthD-1). Exposure to marketed PMB resulted in significantly elevated levels (P < 0.05) of four key biomarkers (KIM-1, cystatin C, clusterin, and OPN) compared to VRP-034, particularly at clinically relevant concentrations of ≥10 µM. At 25 µM, all biomarkers demonstrated a significant increase (P < 0.05) with marketed PMB exposure compared to VRP-034. Inflammatory markers (interleukin-6 and interleukin-8) increased significantly (P < 0.05) with marketed PMB at concentrations of ≥5 µM, relative to VRP-034. VRP-034 displayed superior cell health outcomes, exhibiting lower lactate dehydrogenase release, while ATP levels remained comparable. Morphological analysis revealed that marketed PMB induced more severe damage, disrupting tubular integrity. Both treatments activated cytochrome C, caspase-3, caspase-8, caspase-9, and caspase-12 in a concentration-dependent manner; however, caspase activation was significantly reduced (P < 0.05) with VRP-034. This study demonstrates that VRP-034 significantly reduces nephrotoxicity compared to marketed PMB within a 3D microphysiological system, suggesting its potential to enable the use of full therapeutic doses of PMB with an improved safety profile, addressing the need for less nephrotoxic polymyxin antibiotics.

O objetivo deste relato é compartilhar uma experiência sobre a prática de Consultoria Interna em Gestão de Pessoas, desenvolvida em uma Instituição do Poder Judiciário Estadual. Aborda processos capazes de auxiliar no diagnóstico de necessidades e subsidiar os gestores na condução de ações relativas ao trabalho de acompanhamento funcional de Servidores públicos e gerenciamento de Equipes, bem como propor iniciativas voltadas ao desenvolvimento pessoal e profissional dos Colaboradores, difundindo atenção particular às questões pertinentes à comunicação e liderança, trabalho em equipe, gestão de conflitos, criatividade e inovação, dentre outros aspectos importantes que interferem no ambiente laboral e, consequentemente, no desempenho funcional e resultados da organização. Trata-se de estudo descritivo, realizado a partir dos dados institucionais, bem como pela análise, observação e reflexão da experiência de profissionais que integram a equipe da Unidade Organizacional responsável pelo trabalho de Consultoria Interna. Durante os últimos cinco anos, foram apreciados mais de mil casos no referido Programa, que apontam os desafios e possibilidades relativas à tomada de decisões, facilitação de processos e desenvolvimento de equipes. Convém destacar os esforços pertinentes à promoção de mudanças que influenciam a política, organização, procedimentos e métodos no contexto da Gestão de Pessoas. Os resultados mostram que a prática da Consultoria Interna propicia senso de maior proximidade e ajuda a solucionar problemas complexos no ambiente de trabalho, de forma criativa e colaborativa, fortalecendo e otimizando a Gestão de pessoas e o bem-estar na esfera do Serviço Público e, consequentemente, a eficácia da prestação jurisdicional.

The COVID-19 outbreak has brought about major health crises globally and the persistent nature of long COVID has put a significant strain on hospitals as a result of scarce healthcare resources. Heart problems are discovered to be the most prevalent among persons who have recovered from COVID-19 among several long-lasting effects (Gupta et al., 2021) . There are so many cases of what is generally occurring with post-COVID patients. This work tries to find out the proper machine learning methods to predict the post-COVID complexities which can provide the highest accuracy. The true objective of this endeavor is to conduct additional machine learning-based research in this area of medical science and to develop an accurate machine-learning model. In the case of COVID patients, there are a lot of outcomes in a post-COVID condition. Proper problem definition, data acquisition, Model selection, evaluation of training and testing models, etc., are also significant obstacles to constructing a decent prediction model. There were proposed machine learning models according to previous research which model is combined with multiple models. This research might contribute to making further approaches to predicting the complexities of other diseases like COVID-19.

IgE-mediated hypersensitivity reactions (HRs) to local anaesthetics are extremely uncommon. One of the most widely used local anaesthetics for minor dental and other surgical procedures is lidocaine. The incidence of anaphylactic reaction by Inj. Lidocaine among global population is &amp;#60;1%. The first anaphylactic reaction reported in 1902. A 69-year-old female patient shifted to community hospital emergency department. She presented with complaints of vertigo, anxiety, tachypnoea, hypoxia, and sudden onset of breathlessness. History revealed on administration of Inj. Lidocaine a local anaesthesia to her before knee surgery developed above-mentioned symptoms after 15 minutes. After receiving her from OT patient vitals observed as pulse rate-97bpm, GRBS-140mg/dl, blood pressure-170/70mmHg, spo95% with 0. On emergency basis treated her with anti-histamines (Inj. Chlorpheniramine), steroids (Inj. Hydrocortisone-400mg, Inj.solumedrol-1gm), oxygen support of 7 to 8 lit/min, after 4 days she recovered completely and discharged. We used a scale (Naranjo scale) to evaluate the severity of the adverse drug reaction as per scale, score was five that indicates probability of happening adverse reaction. We conclude the case as anaphylactic reaction to lidocaine could have been cause of the event. It is important to collect patients complete past medical history and their allergic history. Also by giving importance to drug sensitivity testing at least to the list of drugs that are reported to show allergic reactions or anaphylactic reactions in post marketing surveillance. Optimised management protocols can save both the patient life and dilemma faced by physicians.

Despite the crucial role of Polymyxin-B in treating life-threatening gram-negative infections, its clinical utility is limited due to the risk of acute kidney injury. In response, a novel formulation of polymyxin-B is being developed to mitigate drug-induced kidney injury. In this study, we have assessed the toxicity of four variants of that novel formulation (VRP034_F21-F24) in comparison with standard polymyxin-B using kidney injury biomarkers in rats. Sprague-Dawley rats were subcutaneously administered either polymyxin-B (control) or one of the four polymyxin-B formulations at a dose of 25 mg/kg/day (HED: 4 mg/kg/day) in four divided doses for two days. Serum samples were collected at baseline and at the end of day 2 for the determination of serum biomarkers. Necropsy was done on day 2 and kidney was collected for histopathological evaluation. In the control group, statistically significant increase (p < 0.0001) in all biomarkers was observed on day 2 as compared to baseline values [urea: 311%; creatinine: 700%; KIM-1: 180%; cystatin-C: 66%] and 50% of the animals died (one after the 7th dose and two after the 8th dose) before scheduled necropsy. In contrast, animals treated with novel formulations did not show a significant increase across any of the biomarkers and no mortality was observed. Histopathology of the control group kidney confirmed necrotic changes in tissues with congestion and vacuolization, whereas only minor tubular damage was noted in two formulation groups (VRP034_F21, F24) and no appreciable damage was detected in the other two groups (VRP034_F22-23). The novel formulation of polymyxin-B tested in this study significantly reduced the risk of polymyxin-induced kidney injury in rats.

A healthy female infant was born from a twin pregnancy with an isolated congenital lower extremity malformation. Aside from prenatally diagnosed polyhydramnios, the infant had normal prenatal and postnatal diagnostic workup. She underwent transfemoral amputation and healed uneventfully. Congenital limb anomalies may be the result of an unidentified amniotic band, thromboembolic event or twin–twin transfusion syndrome, though in this case, prenatal screening did not indicate any evidence of a limb anomaly and postnatal workup was negative.

We present an unusual case of a 27 year-old-female with undiagnosed mental illness who presented with pricking type of tenderness in and around umbilicus and lower left quadrant of left breast. X-ray revealed the presence of sewing needles on those areas. The foreign body was inserted deliberately by the patient herself on trying to mimic the FNAC procedure performed on her a day before. Under C-arm guidance both the needles were located and extracted. Insertion of foreign body via abdominal wall is the first published report to our knowledge. Performing an invasive procedure in front of a mentally challenged individual requires precaution as a clinician, is an important lesson learned. Birat Journal of Health SciencesVol.2/No.3/Issue 4/Sep- Dec 2017, Page: 303-305

Self-assembly: motivation for constructing ensemble of nanostructures Self-assembly is the autonomous organization of components into patterns or structures. It is ubiquitously present in nature where complex and functional structures are generated from simple building blocks. At the moment, self-assembly has become the most extensively used phenomenon for creating ensemble of nanostructures. Many self-assembling systems have already been designed and investigated. Organic polymers, carbo hydrates, nucleic acids, proteins and small molecules are being used for the construction of selfassembling nanosystems towards various applications ranging from devices to delivery systems [1]. Peptides, in particular, have gained a considerable attention as templates for self-assembly, owing to their inherent biocompatibility, unique physical and chemical properties, easy tunability and ease of functionalization [2]. Moreover, their ubiquitous presence in the biological world makes the peptide-based nanostructures biocompatible and less toxic as compared to their inorganic and metallic counterparts [3]. As detailed structural and functional information is available for an almost endless variety of peptides and proteins, their use as building blocks is very striking. For example, peptides can be designed to adopt well-defined helical and β-hairpin/sheet-like secondary structures that can be utilized as topologically defined building blocks. The chemical synthesis of peptides is rapid and allows a near infinite palette of structure and functions if noncoded residues are used in addition to naturally occurring amino acids.