Automatic White Blood Cells Counting Using OPENCV

The aim of this work was to determine the role of intraperitoneally-administered vitamin E and selenium on the biochemical and haematological parameters in the blood of rats. Thirty-two adult male Wistar rats were used in this study. All rats were randomly divided into four groups. The first group was used as the control. The second group was intraperitoneally administered with vitamin E (+/-(-)alpha-tocopheroryl acetate, 10 mg day-1), the third group with Se (Na2SeO3 0.2 mg over a day), and the fourth group with vitamin E and Se (vitamin E 10 mg + Na2SeO3 0.2 mg over a day). This administration was done for 5 weeks. Blood samples were taken from animals at the end of the dosage period and biochemical parameters in serum samples and haematological parameters in total blood were determined. The levels of total cholesterol (p < 0.01) and number of white blood cell (p < 0.001) in blood were significantly higher in the vitamin E group than in the control group. The levels of ALP, total cholesterol (p < 0.01) and number of white blood cells (p < 0.01) in blood were significantly higher in the selenium group than in the controls. The levels of glucose (p < 0.05), ALP (p < 0.01), total cholesterol (p < 0.001) and number of white blood cells (p < 0.01) were higher in the vitamin E and selenium combined group than in the controls. Other parameters considered within this trial (ALT, LDH, creatinine, albumin, total protein, amylase, creatine kinase, HDL triglycerides, total lipid, sodium, chloride, uric acids, red blood cell, haemoglobin, packed cell volume, MCV, MCH, MCHC) did not show statistically significant differences between the control and injected groups. The results indicated that blood glucose and total cholesterol levels, ALP activity and white blood cell counts were significantly increased by intraperitoneal administration of vitamin E and selenium in rats.

Dengue hemorrhagic fever (DHF) is a public health problem in Indonesia whose number of cases continues to increase and its spread is increasingly widespread.Tests used to diagnose dengue fever include a white blood cell count test.White blood cells are cells that play a role in the body's defense system. The reason is, white blood cells help fight infections in the body, such as viruses, bacteria, parasites and fungi. In patients infected with dengue fever, the white blood cell count is usually normal but then decreases (leukopenia) and neutropenia becomes dominant. Thisresearch was conducted from March to April 2023.The aim of this study was to measure the number of white blood cells in dengue fever patients.This type of research is descriptive. The research was carried out using an automatic analysis method (blood analysis) in the laboratory ofKlinik Dr.Trisna Garut, using samples from 30 dengue fever patients who were tested. In this study, the number of white blood cells in dengue fever patients at Klinik Dr.Trisna Garut was 14 (46.7%).Wich is Normal And the white blood cell count results of dengue fever patients at Klinik Dr.Tisna Garut General Hospital decreased by 16 (54.3%).

The Effect of Uteroferrin and Recombinant GM-CSF on Hematopoietic Parameters in Normal Female Pigs (Sus scrofa)

BackgroundElevated total white blood cell (WBC) count is associated with an increased risk of coronary heart disease and death. Aerobic exercise is associated with lower total WBC, neutrophil, and monocyte counts. However, no studies have evaluated the effect of the amount of aerobic exercise (dose) on total WBC and WBC subfraction counts.PurposeTo examine the effects of 3 different doses of aerobic exercise on changes in total WBC and WBC subfraction counts and independent effects of changes in fitness, adiposity, markers of inflammation (IL-6, TNF-α, C-reactive protein), fasting glucose metabolism, and adiponectin.MethodsData from 390 sedentary, overweight/obese postmenopausal women from the DREW study were used in these analyses. Women were randomized to a non-exercise control group or one of 3 exercise groups: energy expenditure of 4, 8, or 12 kcal kg−1⋅week−1 (KKW) for 6 months at an intensity of 50% VO2peak.ResultsA dose-dependent decrease in total WBC counts (trend P = 0.002) was observed with a significant decrease in the 12KKW group (−163.1±140.0 cells/µL; mean±95%CI) compared with the control (138.6±144.7 cells/µL). A similar response was seen in the neutrophil subfraction (trend P = 0.001) with a significant decrease in the 12KKW group (−152.6±115.1 cells/µL) compared with both the control and 4KKW groups (96.4±119.0 and 21.9±95.3 cells/µL, respectively) and in the 8KKW group (−102.4±125.0 cells/µL) compared with the control. When divided into high/low baseline WBC categories (median split), a dose-dependent decrease in both total WBCs (P = 0.003) and neutrophils (P<0.001) was observed in women with high baseline WBC counts. The effects of exercise dose on total WBC and neutrophil counts persisted after accounting for significant independent effects of change in waist circumference and IL-6.ConclusionAerobic exercise training reduces total WBC and neutrophil counts, in a dose-dependent manner, in overweight/obese postmenopausal women and is especially beneficial for those with systemic low grade inflammation.Clinical Trials Identifier: NCT00011193

The acute effects of dihydrocapsaicin (DHC) and capsaicin (CAP) on the number of white blood cells (WBCs), neutrophils, eosinophils, basophils, monocytes, lymphocytes, T lymphocytes, B lymphocytes and NK cells, and serum corticosterone levels were studied in rats. Male 7-wk-old SD rats were divided into DHC (3.0 mg/kg BW), CAP (3.0 mg/kg BW) and control (CON) groups. The number of total WBCs was 1.30-1.42 times significantly higher in the DHC group than in the CON group at 6-12 h. The number of neutrophils was 1.62 times significantly higher in the DHC group than in the CON group at 12 h. The number of total WBCs and neutrophils, however, showed no significant changes between the CAP and CON groups. The number of lymphocytes was 0.61 and 0.70 times significantly lower in the DHC and CAP groups than in the CON group at 3 h. The number of T lymphocytes and B lymphocytes was 0.74 and 0.54 times lower in the DHC group than in the CON group, respectively. CAP, however, did not significantly change the number of T lymphocytes or B lymphocytes. No significant changes in the number of NK cells were observed among the three groups. CAP and DHC did not change the number of monocytes, eosinophils or basophils. No significant changes of the serum corticosterone levels were observed among the three groups. In conclusion, capsaicinoids decreased the number of acquired immunity cells, and increased the number of total WBCs and neutrophils without changing the number of monocytes, eosinophils or basophils. The magnitude of these effects was relatively higher in DHC than in CAP.

Thirteen patients with chronic granulocytic leukemia were given 28 separate irradiations to the spleen for the treatment of their disease. The radiation was given as a single dose that ranged between 100 and 700 rads. The changes in the circulating white blood cell (WBC) count could be represented by the expression $N=B_{0}+S_{0}(e^{-\beta t})$ where N is the number of WBC per mm3 of blood at day t; S0 is the number of WBC, at time zero, originating from the spleen; and B0 is the number of WBC stemming from nonirradiated parts of the body, mostly bone marrow. The disappearance rate β, determined by an indirect method, indicated that S0 cells were leaving the blood with a half-time of 4.5 days. The number of S0 cells could be calculated a posteriori by the expression $S_{0}=(N_{0}-N)/(1-e^{-\beta t})$, where N0 is the number of WBC before spleen irradiation and N the number of WBC at t days after irradiation.

Objective To investigate the relationship between vitamin D, fibrinogen, white blood cell count and hypertension, find out the correlation of vitamin D and fibrinogen. Methods Oct.2010 to Oct.2012 hospitalised 326 hypertensive patients and 218 healthy cases were involved. To analysis the differences of fibrinogen, vitamin D and the number of white blood cells status. Then research the correlation of vitamin D and fibrinogen in hypertension group. Results Compared with the hypertension group and the non-high-blood group, vitamin D, fibrinogen, the number of white blood cells, lymphocytes, neutrophils and lipids, the difference was statistically significant (P &lt; 0.05). To analysis the multiple factors regression, for hypertension, vitamin D is protective factor (B value = 0.186, OR value = 0.83, 95% CI0.795–0.869), but neutrophils, fibrinogen, low density lipoprotein are risk factors (B value were 0.761, 0.173, 0.987, OR value were 2.141, 1.189, 2.128, 95% CI1.445–3.1731, 1.14 1.908, 1.84 3.971). Hypertension group vitamin D and fibrinogen have negative correlation (r = 0.152, P = 0.011). Conclusions The vitamin D level of hypertension group was higher and the fibrinogen level was lower than the non-hypertensive group. For hypertension, vitamin D is protective factor, while neutrophils and fibrinogen are risk factors. Vitamin D and fibrinogen were negatively correlated in hypertension patients.

The purpose of this study is to see how low-power red laser helium-neon and diode lasers with wavelengths of 632.8 nm and 650 nm, respectively, affect some human blood sample factors like viscosity HCT, Platelets PLT, White blood cells WBC, white blood lymphocytes LYM, neutrophilic white blood cells NEUT, red blood cells RBC, and Erythrocyte Sedimentation Rate ESR. Blood samples were collected from healthy people and placed in EDTA-contained tubes, blood tests were performed on control samples in a blood analyzer, and the samples were divided into two parts and split into two equal tubes to be irradiated with diode and helium-neon lasers for 15 minutes. Using a blood analyzer, measurements were taken immediately after irradiation. The results of this analysis indicated a decrease in the number of white blood cells (WBC), red blood cells (RBC), and blood viscosity (HCT), as well as a significant rise in the number of neutrophils (NEUT) and platelets (PLT) and increase in the Erythrocyte Sedimentation Rate ESR after exposure to both helium-neon and diode lasers. This suggests that It is possible to make recommendations, a low-power laser could be used to stimulate blood cells so a laser could be used to treat blood viscosity. Because of the increasing concentrations of free intracellular Calcium ions and the action of laser on the plasma composition, it is acceptable to suggest that laser irradiation can lower the number of white blood cells (WBC), red blood cells (RBC), and the reason of that is because of a mechanical change in the blood such as a change in the pores of the surfaces and their fixation.

The recent study is done in the animal house of college of Education,Biological sciences Department .This is study aimed to know three concentration of honey with their effects on some of the blood pictures representing (PCV, Hb, RBCs, WBCs)by drawing 2-3 ml from the white rats eye angle at 9 o’clock in the morning .Rats are divided into four groups. The first is control group,The second group is feeding with honey at concentration 50%,The third with honey at concentration 75%,The fourth with honey at concentration 100%, all this groups feeding twice in 24 hours .They divided into groups, the first feeds with honey for one week,the second feeds for two weeks. The results were as following :In first week as showed in the first table for count of white blood cells,It is noticed there are no significant differences,In the second week, as illustrated in first table for white blood cells, It is observed there are no differences in number of white blood cells .In the first week,as showed in the second table. It is noticed increasing in Hemoglobin level for treatment of concentration 100%,In second week, it is noticed there is no increase of hemoglobin level in all treatment .In the first week, in the third table there is increasing in counts of the Red blood corpuscles. At concentration 100%.In the first week,as showed in the fourth table It is noticed there is no significant in size of PCV in the first and the second week.

In December 2019, a novel coronavirus was identified in Wuhan City, Hubei Province, China and later the disease was named coronavirus disease 2019 (COVID-19). On March 11, 2020, the World Health Organization (WHO) officially announced that COVID-19 had reached global pandemic status. This article summarized the understanding of the etiology, pathogenesis, epidemiology, clinical characteristics, diagnosis, treatment, rehabilitation, and prevention and control measures of COVID-19 based on the available data and anti-epidemic experience in China.

The purpose of this experiment was to investigate the impact of laser radiation on certain blood characteristics of students. Blood samples were collected from healthy individuals who were normal students, under standard laboratory conditions. A total of twenty healthy were obtained. Each sample was divided into three parts, with one part serving as a control (untreated) and the other two parts being exposed to laser radiation with power levels of 0.3mW and 0.9mW. The blood samples were irradiated using a continuous helium-neon laser with a wavelength of 632.8 nm for a duration of 40 minutes. Blood analysis was conducted using a complete blood count (CBC) device, and the results of the exposed samples were compared to the control samples. After 40 minutes, the CBC analysis showed changes in the components and characteristics of the blood. The number of white blood cells significantly increased with higher power levels, and there was also an increase in the number of platelets. Random fluctuations in the sedimentation rate of hemoglobin and red blood cells were observed. However, statistical analysis of the average counts of white blood cells, red blood cells, and platelets for all groups indicated that the observed increases were not statistically significant (p &gt; 0.05). This emphasizes the importance of low-power lasers in various biomedical applications for treating different illnesses. The study concluded that laser radiation altered the biological components of the irradiated samples.

Effects of compound lobelia oral liquid on acute pharyngitis rabbits model

A leukocyte and erythrocyte adhesiveness/aggregation test to reveal the presence of smoldering inflammation and risk factors for atherosclerosis

Lintah laut (hirudinea: Zeylanicobdella arugamensis) merupakan ektoparasit jenis yang dapat menginfeksi dan menghambat pertumbuhan ikan budidaya. Tujuan dari penelitian ini untuk mengetahui tingkat infeksi lintah laut serta gambaran darah ikan kerapu hibrida (Epinephelus sp.) dan ikan kakap putih (Lates calcarifer) melalui metode kohabitasi. Penelitian ini menggunakan 94 ekor ikan kerapu hibrida (61 ekor ukuran kecil: 14,48±1,14 cm dan 33 ekor ukuran besar: 37,18±18,46 cm ) dan 76 ekor ikan kakap putih (46 ekor ukuran kecil: 13,7±1,04 cm dan 30 ekor ukuran besar: 31,87±5,78 cm). Uji kohabitasi dilakukan dengan menempatkan Z. arugamensis ukuran 1-2,5 cm (206-392 individu/perlakuan) ke dalam bak fiber volume 100 L yang telah diisi ikan kakap putih, kerapu hibrida cantik dan kombinasi kedua ikan tersebut. Perlakuan kontrol menggunakan ikan kakap putih dan kerapu hibrida cantik tanpa penambahan Z. arugamensis. Intensitas Z. arugamensis per ikan dihitung setelah 2 minggu kohabitasi. Hasil penelitian menunjukkan bahwa intensitas dan prevalensi lintah laut pada ikan kerapu cantik (intensitas: 18-59 lintah/ikan ukuran kecil dan 50-313 lintah/ikan ukuran besar, dengan prevalensi 90-100%) lebih tinggi dibandingkan dengan ikan kakap putih (intensitas: 1-8 lintah/ikan ukuran kecil dan 3-12 lintah/ikan ukuran besar, dengan prevalensi 41-100%). Profil darah ikan kerapu hibrida yang terinfeksi Z. arugamensis menunjukkan jumlah sel darah putih, ukuran rata-rata sel darah merah (MCV) dan jumlah rata-rata hemoglobin di dalam sel darah merah (MCH) lebih tinggi dibandingkan dengan jumlah sel yang sama pada ikan kerapu hibrida yang tidak terinfeksi Z. arugamensis. Namun profil darah ikan kakap putih hasil kohabitasi dengan Z. arugamensis dengan kakap putih dari kelompok kontrol hampir sama. Hasil tersebut menunjukkan ikan kerapu hibrida lebih rentan terhadap infeksi Z. arugamensis dibandingkan dengan ikan kakap putih.Sea leech (Hirudinea: Zeylanicobdella arugamensis) is a type of ectoparasites that can infect and inhibit the growth of cultivated fish. The purpose of this study was to determine the infection rate of marine leeches and the blood profile of hybrid grouper (Epinephelus sp.) and barramundi (Lates calcarifer) through the cohabitation method. This study used 94 hybrid groupers (61 small size with TL: 14.48±1.14 cm and 33 large size with TL: 37.18±18.46 cm) and 76 barramundi (46 small size with TL: 13 .7±1.04 cm and 30 large size with TL: 31.87±5.78 cm). The cohabitation test was carried out by placing Z. arugamensis with total length of 1-2.5 cm (206-392 individuals/treatment) into a 100 L volume fiber tank filled with barramundi, hybrid grouper and a combination of the two fish. The control treatment used barramundi and hybrid grouper without the addition of Z. arugamensis. The intensity and prevalence of Z. arugamensis per fish was calculated after 2 weeks of cohabitation. The results showed that the intensity and prevalence of marine leeches in hybrid grouper (intensity: 18-59 leeches/small size fish and 50-313 leeches/large size fish, with a prevalence of 90-100%) were higher than the barramundi (intensity : 1-8 leeches/small size fish and 3-12 leeches/large size fish, with a prevalence of 41-100%). The blood profile of hybrid grouper infected with Z. arugamensis showed that the number of white blood cells (WBC), the mean corpuscular volume (MCV) and the mean corpuscular hemoglobin (MCH) were higher than the same number of cells in hybrid grouper that was not infected with Z. arugamensis. However, the blood profile of barramundi from cohabitation with Z. arugamensis and barramundi from the control group were almost the same. These results indicated that hybrid grouper was more susceptible to Z. arugamensis infection compared to barramundi.

The objective of this study was to determine whether exposure to ozone causes inflammatory or functional changes in the upper or lower airways of asthmatic and nonasthmatic individuals. Ten asthmatic and eight nonasthmatic subjects were exposed to clean air, 120 ppb ozone, or 240 ppb ozone for 90 min during intermittent moderate exercise using a head dome exposure system. Pulmonary function tests, posterior rhinomanometry, and nasal lavage were performed before and after exposure. Leukocyte counts and chemotactic factors leukotriene B4 (LTB4), platelet-activating factor (PAF), and interleukin-8 (IL-8) were analyzed from nasal lavage fluid. In subjects with asthma, a significant increase (p < 0.05) in the number of white blood cells in lavage fluid was detected both immediately and 24 h after exposure to 240 ppb ozone, as was a significant increase in epithelial cells immediately after exposure (p < 0.05). No significant cellular changes were seen in nonasthmatic subjects. A significant correlation was observed between IL-8 and white blood cells counts after exposure to 240 ppb ozone (r = 0.76) in asthmatic subjects. No significant changes in pulmonary or nasal function or biochemical mediators were found in either the asthmatic or nonasthmatic subjects. These data indicate that asthmatic individuals are more sensitive to the acute inflammatory effects of ozone than nonasthmatic individuals.