Splenic Irradiation in Chronic Granulocytic Leukemia: Changes in Leukocyte Values

The acute effects of dihydrocapsaicin (DHC) and capsaicin (CAP) on the number of white blood cells (WBCs), neutrophils, eosinophils, basophils, monocytes, lymphocytes, T lymphocytes, B lymphocytes and NK cells, and serum corticosterone levels were studied in rats. Male 7-wk-old SD rats were divided into DHC (3.0 mg/kg BW), CAP (3.0 mg/kg BW) and control (CON) groups. The number of total WBCs was 1.30-1.42 times significantly higher in the DHC group than in the CON group at 6-12 h. The number of neutrophils was 1.62 times significantly higher in the DHC group than in the CON group at 12 h. The number of total WBCs and neutrophils, however, showed no significant changes between the CAP and CON groups. The number of lymphocytes was 0.61 and 0.70 times significantly lower in the DHC and CAP groups than in the CON group at 3 h. The number of T lymphocytes and B lymphocytes was 0.74 and 0.54 times lower in the DHC group than in the CON group, respectively. CAP, however, did not significantly change the number of T lymphocytes or B lymphocytes. No significant changes in the number of NK cells were observed among the three groups. CAP and DHC did not change the number of monocytes, eosinophils or basophils. No significant changes of the serum corticosterone levels were observed among the three groups. In conclusion, capsaicinoids decreased the number of acquired immunity cells, and increased the number of total WBCs and neutrophils without changing the number of monocytes, eosinophils or basophils. The magnitude of these effects was relatively higher in DHC than in CAP.

The Effect of Uteroferrin and Recombinant GM-CSF on Hematopoietic Parameters in Normal Female Pigs (Sus scrofa)

Dengue hemorrhagic fever (DHF) is a public health problem in Indonesia whose number of cases continues to increase and its spread is increasingly widespread.Tests used to diagnose dengue fever include a white blood cell count test.White blood cells are cells that play a role in the body's defense system. The reason is, white blood cells help fight infections in the body, such as viruses, bacteria, parasites and fungi. In patients infected with dengue fever, the white blood cell count is usually normal but then decreases (leukopenia) and neutropenia becomes dominant. Thisresearch was conducted from March to April 2023.The aim of this study was to measure the number of white blood cells in dengue fever patients.This type of research is descriptive. The research was carried out using an automatic analysis method (blood analysis) in the laboratory ofKlinik Dr.Trisna Garut, using samples from 30 dengue fever patients who were tested. In this study, the number of white blood cells in dengue fever patients at Klinik Dr.Trisna Garut was 14 (46.7%).Wich is Normal And the white blood cell count results of dengue fever patients at Klinik Dr.Tisna Garut General Hospital decreased by 16 (54.3%).

The aim of this work was to determine the role of intraperitoneally-administered vitamin E and selenium on the biochemical and haematological parameters in the blood of rats. Thirty-two adult male Wistar rats were used in this study. All rats were randomly divided into four groups. The first group was used as the control. The second group was intraperitoneally administered with vitamin E (+/-(-)alpha-tocopheroryl acetate, 10 mg day-1), the third group with Se (Na2SeO3 0.2 mg over a day), and the fourth group with vitamin E and Se (vitamin E 10 mg + Na2SeO3 0.2 mg over a day). This administration was done for 5 weeks. Blood samples were taken from animals at the end of the dosage period and biochemical parameters in serum samples and haematological parameters in total blood were determined. The levels of total cholesterol (p < 0.01) and number of white blood cell (p < 0.001) in blood were significantly higher in the vitamin E group than in the control group. The levels of ALP, total cholesterol (p < 0.01) and number of white blood cells (p < 0.01) in blood were significantly higher in the selenium group than in the controls. The levels of glucose (p < 0.05), ALP (p < 0.01), total cholesterol (p < 0.001) and number of white blood cells (p < 0.01) were higher in the vitamin E and selenium combined group than in the controls. Other parameters considered within this trial (ALT, LDH, creatinine, albumin, total protein, amylase, creatine kinase, HDL triglycerides, total lipid, sodium, chloride, uric acids, red blood cell, haemoglobin, packed cell volume, MCV, MCH, MCHC) did not show statistically significant differences between the control and injected groups. The results indicated that blood glucose and total cholesterol levels, ALP activity and white blood cell counts were significantly increased by intraperitoneal administration of vitamin E and selenium in rats.

BackgroundElevated total white blood cell (WBC) count is associated with an increased risk of coronary heart disease and death. Aerobic exercise is associated with lower total WBC, neutrophil, and monocyte counts. However, no studies have evaluated the effect of the amount of aerobic exercise (dose) on total WBC and WBC subfraction counts.PurposeTo examine the effects of 3 different doses of aerobic exercise on changes in total WBC and WBC subfraction counts and independent effects of changes in fitness, adiposity, markers of inflammation (IL-6, TNF-α, C-reactive protein), fasting glucose metabolism, and adiponectin.MethodsData from 390 sedentary, overweight/obese postmenopausal women from the DREW study were used in these analyses. Women were randomized to a non-exercise control group or one of 3 exercise groups: energy expenditure of 4, 8, or 12 kcal kg−1⋅week−1 (KKW) for 6 months at an intensity of 50% VO2peak.ResultsA dose-dependent decrease in total WBC counts (trend P = 0.002) was observed with a significant decrease in the 12KKW group (−163.1±140.0 cells/µL; mean±95%CI) compared with the control (138.6±144.7 cells/µL). A similar response was seen in the neutrophil subfraction (trend P = 0.001) with a significant decrease in the 12KKW group (−152.6±115.1 cells/µL) compared with both the control and 4KKW groups (96.4±119.0 and 21.9±95.3 cells/µL, respectively) and in the 8KKW group (−102.4±125.0 cells/µL) compared with the control. When divided into high/low baseline WBC categories (median split), a dose-dependent decrease in both total WBCs (P = 0.003) and neutrophils (P<0.001) was observed in women with high baseline WBC counts. The effects of exercise dose on total WBC and neutrophil counts persisted after accounting for significant independent effects of change in waist circumference and IL-6.ConclusionAerobic exercise training reduces total WBC and neutrophil counts, in a dose-dependent manner, in overweight/obese postmenopausal women and is especially beneficial for those with systemic low grade inflammation.Clinical Trials Identifier: NCT00011193

Summary.Account of 24 cases of acute leukemia in children.The acute leukemia in children as a rule is a myeloblast‐leuke‐mia (in at least 21 of 24 cases).The initial symptoms are diffuse, – fatigue, fever, lack of appetite and pallor. In most cases (23 of 24) there is found either splenic or glandular enlargement and besides, in 13 cases also enlargement of the liver. These symptoms are considered as specially valuable in an early diagnosis.3/4 of the cases commence in the first 5 years of life.Anemia is a frequent symptom in an early stage of the disease, but in some cases it may be absent for weeks.The red blood corpuscles show no characteristics. Index varies between 0.7 and 1.8. In 20 out of 23 cases index lies between 0.8 and 1.2.Clinical bleeding‐tendency is found in 50 % of the patients in the more advanced stages of the disease. No connection between the bleeding‐tendency and the number of thrombocytes has been demonstrated.The number of white blood cells varies within wide limits, from 1300 to 185 000 per mm3 of blood. It also varies highly from time to time in the individual patient. On admission to the department, 21 of 24 patients show values below 20 000 white blood cells per mm3 of blood, 17 below 10 000. 2 patients showed above 30 000 white blood cells per mm3 of blood.Eoentgen picture of the bones have been taken in 17 patients. 5 show certain changes similar to those that sometimes are found in acute leukemia.In 18 cases it has been made successful sternal puncture with findings of bone marrow rich in cells, with up to 99.4 % of myeloblasts. The pathological cellular type has in all cases been demonstrated in blood‐smear preparations, in 17 of 18 cases however, they have been recognised as myeloblasts only after successful sternal puncture having ascertained the diagnosis.

Total white blood cell counts and LPS-induced TNFα production by monocytes of pregnant, pseudopregnant and cyclic rats

An open-label, randomized trial of the effects of macrophage versus granulocyte colony-stimulating factor on natural killer cell activity, lymphokine activated killer cell activity, interleukin-2 production, and leukocyte count in patients after chemotherapy for ovarian cancer

Objective To investigate the relationship between vitamin D, fibrinogen, white blood cell count and hypertension, find out the correlation of vitamin D and fibrinogen. Methods Oct.2010 to Oct.2012 hospitalised 326 hypertensive patients and 218 healthy cases were involved. To analysis the differences of fibrinogen, vitamin D and the number of white blood cells status. Then research the correlation of vitamin D and fibrinogen in hypertension group. Results Compared with the hypertension group and the non-high-blood group, vitamin D, fibrinogen, the number of white blood cells, lymphocytes, neutrophils and lipids, the difference was statistically significant (P &lt; 0.05). To analysis the multiple factors regression, for hypertension, vitamin D is protective factor (B value = 0.186, OR value = 0.83, 95% CI0.795–0.869), but neutrophils, fibrinogen, low density lipoprotein are risk factors (B value were 0.761, 0.173, 0.987, OR value were 2.141, 1.189, 2.128, 95% CI1.445–3.1731, 1.14 1.908, 1.84 3.971). Hypertension group vitamin D and fibrinogen have negative correlation (r = 0.152, P = 0.011). Conclusions The vitamin D level of hypertension group was higher and the fibrinogen level was lower than the non-hypertensive group. For hypertension, vitamin D is protective factor, while neutrophils and fibrinogen are risk factors. Vitamin D and fibrinogen were negatively correlated in hypertension patients.

Counting the number of white blood cells (WBCs) is a crucial procedure in medical laboratories for diagnosing various diseases. However, manual counting can be time-consuming and susceptible to errors. To overcome this, a research study has proposed an automated approach for WBC counting in sampled images using OpenCV, an open-source computer vision library. The authors developed an algorithm that segments the WBCs from the background by utilizing preprocessing techniques, followed by edge detection (canny edge detection) to identify the cells' boundaries. The number of cells is counted by implementing a simple circular Hough transform method. For this, the authors approached and collected datasets from ALL-IDB team for sampled images to test the proposed method. The proposed method has achieved high accuracy rates and outperformed manual counting in terms of speed and efficiency. The developed approach has the potential to be integrated into existing medical laboratory workflows, automating the WBC counting process and improving the diagnosis and treatment of various diseases.

The recent study is done in the animal house of college of Education,Biological sciences Department .This is study aimed to know three concentration of honey with their effects on some of the blood pictures representing (PCV, Hb, RBCs, WBCs)by drawing 2-3 ml from the white rats eye angle at 9 o’clock in the morning .Rats are divided into four groups. The first is control group,The second group is feeding with honey at concentration 50%,The third with honey at concentration 75%,The fourth with honey at concentration 100%, all this groups feeding twice in 24 hours .They divided into groups, the first feeds with honey for one week,the second feeds for two weeks. The results were as following :In first week as showed in the first table for count of white blood cells,It is noticed there are no significant differences,In the second week, as illustrated in first table for white blood cells, It is observed there are no differences in number of white blood cells .In the first week,as showed in the second table. It is noticed increasing in Hemoglobin level for treatment of concentration 100%,In second week, it is noticed there is no increase of hemoglobin level in all treatment .In the first week, in the third table there is increasing in counts of the Red blood corpuscles. At concentration 100%.In the first week,as showed in the fourth table It is noticed there is no significant in size of PCV in the first and the second week.

Since blood cells count is the most important indicator of animals' physiological status, we investigated the effects of age, litter size, and multiple paternity on the total number of white blood cells, the number of their individual types (lymphocytes, neutrophils, eosinophils, monocytes), and the ratio of neutrophils to lymphocytes in domestic cat during early postnatal ontogenesis. The study was conducted on kittens living in outdoor conditions, aged from birth to 6 months. The number of white blood cells was evaluated using a hemoanalyzer, the leukocyte formula and the proportion of cell types were determined manually from blood smears. Age significantly affected the number of leukocytes in the first 3 months of kittens' age. The number and proportion of neutrophils were the highest after birth and gradually decreased during the first month. Lymphocytes number, on the contrary, increased during this period. Monocytes and eosinophils increased in number in the first 2 months. The litter size affected the number of leukocytes and neutrophils in the first 2 months of life, their number being significantly higher in kittens from the small litters than from the large ones. In kittens from the litters with multiple paternity, the number of leukocytes and the proportion of neutrophils was higher than in litters from a single male. Thus, age, litter size and type of paternity may affect the hematological indices in domestic cats, which must be taken into account during the estimation of the health status of kittens in domestic and wild cats.

The purpose of this study is to see how low-power red laser helium-neon and diode lasers with wavelengths of 632.8 nm and 650 nm, respectively, affect some human blood sample factors like viscosity HCT, Platelets PLT, White blood cells WBC, white blood lymphocytes LYM, neutrophilic white blood cells NEUT, red blood cells RBC, and Erythrocyte Sedimentation Rate ESR. Blood samples were collected from healthy people and placed in EDTA-contained tubes, blood tests were performed on control samples in a blood analyzer, and the samples were divided into two parts and split into two equal tubes to be irradiated with diode and helium-neon lasers for 15 minutes. Using a blood analyzer, measurements were taken immediately after irradiation. The results of this analysis indicated a decrease in the number of white blood cells (WBC), red blood cells (RBC), and blood viscosity (HCT), as well as a significant rise in the number of neutrophils (NEUT) and platelets (PLT) and increase in the Erythrocyte Sedimentation Rate ESR after exposure to both helium-neon and diode lasers. This suggests that It is possible to make recommendations, a low-power laser could be used to stimulate blood cells so a laser could be used to treat blood viscosity. Because of the increasing concentrations of free intracellular Calcium ions and the action of laser on the plasma composition, it is acceptable to suggest that laser irradiation can lower the number of white blood cells (WBC), red blood cells (RBC), and the reason of that is because of a mechanical change in the blood such as a change in the pores of the surfaces and their fixation.

The aim of this review is to assist non-hematologist effective means of interpreting complete blood cell (CBC) counts results especially when encountered with abnormal result for intellectually rewarding practice and to recognize when a subspecialty consultation is reasonable and when it may be circumvented. A complete blood cell count is routine hematology tests in medicine useful for the differential diagnosis of anemia and other medical conditions. A good percentage of CBC results are reported as abnormal, therefore it is in every clinician’s interest to have some understanding of the specific test basics as well as a structured action plan when confronted with abnormal results. It is a series of tests used to evaluate the composition and concentration of the cellular components of blood, which consists: red blood cell (RBC) counts, red cell indices, hematocrit, hemoglobin concentration, white blood cell (WBC) count, classification of white blood cells (WBC differential) and platelet count. CBC provides valuable information about the blood and to some extent the bone marrow, which is the blood-forming tissue. CBC can be use for the following purposes: as a preoperative test to ensure both adequate oxygen carrying capacity and hemostasis, to identify acute and chronic illness, bleeding tendencies, and white blood cell disorders such as leukemia, to monitor a medical condition and changes in the body system caused by medical treatments, to determine the effects of chemotherapy and radiation therapy on blood cell production. CBC can be performed manually using visual examination or automation by fluorescence flow cytometry and impedance. In conclusion, CBC and peripheral blood examination are one of the most commonly ordered tests that provides important information about the kinds and numbers of cells in the blood, abnormalities in any of these types of blood cells may indicate the presence of important medical disorders.

High Thrombopoietin Production by Hematopoietic Cells Induces a Fatal Myeloproliferative Syndrome in Mice