Fiber motion-insensitive fiber-based polarization-sensitive optical coherence tomography for optic axis determination

We present an improved method of polarization sensitive optical coherence tomography that enables measurement and imaging of backscattered intensity, birefringence, and fast optic axis orientation simultaneously with only one single A-scan per transverse measurement location. While intensity and birefringence data are obtained in a conventional way, the optic axis orientation is determined from the phase difference recorded in two orthogonal polarization channels. We report on accuracy and precision of the method by measuring birefringence and optic axis orientation of well defined polarization states in a technical object and present maps of birefringence and, what we believe for the first time, of optic axis orientation in biological tissue.

Polarization-sensitive optical coherence tomography (PS-OCT) extends OCT by analyzing the polarization states of backscattered light to quantify tissue birefringence. However, conventional implementations require polarization-diverse detection and are therefore incompatible with most commercial OCT systems. As a result, PS-OCT has largely remained restricted to specialized research groups, limiting its broader scientific and clinical use. Here, we present a modular PS-OCT framework that integrates with a standard spectral-domain OCT platform through a detachable rotating achromatic half-wave plate in the sample arm. This waveplate modulates both incident and reflected polarization states. Three or more repeated measurements at distinct waveplate orientations enable reconstruction of the sample's round-trip Jones matrix and the corresponding polarization properties. To mitigate random phase variations between repeated measurements, we introduce a retarder-constrained phase optimization strategy. We validate the framework with imaging of birefringent phantoms and the human retina in vivo, demonstrating reliable reconstruction of retardance and optic axis orientation. This approach requires only minimal hardware modification and is readily deployable on mainstream OCT systems. Lowering technical barriers paves the way for rapid and widespread deployment of PS-OCT across diverse biomedical applications in both research and clinical environments.

The Casimir force is calculated in the configuration of two parallel plates separated by an anisotropic media. The result exhibits a dependence on the orientation of the optical axis of the intervening media. It is possible that the Casimir force switches its direction as the optical axis orientation varies. The greatest magnitude of the force could be achieved at any optical axis orientation, depending on the dielectric properties of the plates and the intervening media. A comparison between the relativistic and nonrelativistic result revealed that the nonrelativistic approximation could significantly underestimate the attraction or overestimate the repulsion. This error was even greater when the optical axis of the intervening media was perpendicular to the surface of the plates. The nonrelativistic approximation might even fail to predict the trends of the Casimir force at small distances.

In this work, a Polarization Sensitive Optical Coherence Tomography (PS-OCT) system is described with the ability of navigation in neurosurgery. Of great importance in neurosurgery is guiding Deep Brain Stimulation (DBS) needle probe which needs precise and detailed navigation within the brain tissue. PS-OCT is a powerful imaging tool that can be used to obtain detailed information within the tissue at different depths based on the low coherent light interferometry and birefringence properties of the tissue by exploiting polarimetric methods. It is noteworthy to mention that PS-OCT exhibits great resolution and sensitivity based on the tissue birefringence related parameters (retardation and optic axis orientation) especially for the precise placement of DBS probe in the subthalamic nucleus (STN), which is entirely surrounded by myelinated fiber bundles that are highly birefringent. In general, images obtained using this system can provide a spatial map of the anisotropic structure of the tissue.

Polarization sensitive optical coherence tomography (PS-OCT) is a functional extension of OCT. In addition to imaging based on tissue reflectivity, PS-OCT also enables depth-resolved mapping of sample polarization properties such as phase-retardation, birefringent axis orientation, Stokes vectors, and degree of polarization uniformity (DOPU). In this study, PS-OCT was used to investigate the polarization properties of melanin. In-vitro measurements in samples with varying melanin concentrations revealed polarization scrambling, i.e. depolarization of backscattered light. Polarization scrambling in the PS-OCT images was more pronounced for higher melanin concentrations and correlated with the concentration of the melanin granules in the phantoms. Moreover, in-vivo PS-OCT was performed in the retinas of normal subjects and individuals with albinism. Unlike in the normal eye, polarization scrambling in the retinal pigment epithelium (RPE) was less pronounced or even not observable in PS-OCT images of albinos. These results indicate that the depolarizing appearance of pigmented structures like, for instance, the RPE is likely to be caused by the melanin granules contained in these cells.

We present fiber-based polarization-sensitive swept-source optical coherence tomography (SS-OCT) based on continuous source polarization modulation. The light source is a frequency swept laser centered at 1.31 microm with a scanning rate of 20 kHz. The incident polarization is modulated by a resonant electro-optic modulator at 33.3 MHz, which is one-third of the data acquisition frequency. The zeroth- and first-order harmonic components of the OCT signals with respect to the polarization modulation frequency have the polarimetric information of the sample. By algebraic and matrix calculations of the signals, this system can measure the depth-resolved Jones matrices of the sample with a single wavelength scan. The phase fluctuations of the starting trigger of wavelength scan and the polarization modulation are cancelled by monitoring the OCT phase of a calibration mirror inserted into the sample arm. We demonstrate the potential of the system by the measurement of chicken breast muscle and the volumetric measurement of an in vivo human anterior eye segment. The phase retardation image shows an additional contrast in the fibrous tissue such as the collagen fiber in the trabecular meshwork and sclera.

Assessing tissue birefringence with imaging modality polarization-sensitive optical coherence tomography (PS-OCT) could improve the characterization of in vivo tissue pathology. Among the birefringent components, collagen may provide invaluable clinical information because of its alteration in disorders ranging from myocardial infarction to arthritis. But the features required of clinical imaging modality in these areas usually include the ability to assess the parameter of interest rapidly and without extensive data analysis, the characteristics that single-detector PS-OCT demonstrates. But beyond detecting organized collagen, which has been previously demonstrated and confirmed with the appropriate histological techniques, additional information can potentially be gained with PS-OCT, including collagen type, form versus intrinsic birefringence, the collagen angle, and the presence of multiple birefringence materials. In part I, we apply the simple but powerful fast-Fourier transform (FFT) to both PS-OCT mathematical modeling and in vitro bovine meniscus for improved PS-OCT data analysis. The FFT analysis yields, in a rapid, straightforward, and easily interpreted manner, information on the presence of multiple birefringent materials, distinguishing the true anatomical structure from patterns in image resulting from alterations in the polarization state and identifying the tissue/phantom optical axes. Therefore the use of the FFT analysis of PS-OCT data provides information on tissue composition beyond identifying the presence of organized collagen in real time and directly from the image without extensive mathematical manipulation or data analysis. In part II, Helistat phantoms (collagen type I) are analyzed with the ultimate goal of improved tissue characterization. This study, along with the data in part I, advance the insights gained from PS-OCT images beyond simply determining the presence or absence of birefringence.

Polarization maintaining (PM) fibers can be used for distributed force/pressure sensing in which the birefringence axis of the PM fiber should preferably be oriented 45° from the direction of the force/pressure for the maximum sensitivity. However, it is a challenge to achieve such 45° axis orientation for a long length of PM fiber in practice. In this paper, we report the development of what we believe the first equipment and process for making PM fiber based sensing tapes, capable of automatically adjusting the fiber axis orientation 45° with respect to the tape surface. In particular, we develop a machine vision system with the ability of continuously determining fiber axis orientation in real time as the fiber passes by and feeding back the orientation information to a fiber rotation apparatus to automatically adjust its orientation before fixing the fiber on a transparent PET tape with UV epoxy. We show the results of a successfully fabricated 70-m-long PM fiber sensing tape achieving an axis orientation accuracy of 45 ± 3° throughout the whole length of the tape, which is further validated with a distributed polarization crosstalk analyzer (DPXA). Finally, we demonstrate distributed transversal load sensing with 14 force applying weights randomly distributed along the sensing tape using the DPXA, with a polarization crosstalk measurement uniformity of 0.62 dB (standard deviation) using the same applied weight of 100 grams. The same sensing tape can also be used for pressure sensing with properly designed fixtures.

To identify and quantify subretinal fibrosis in eyes with advanced neovascular age-related macular degeneration (nAMD) using polarization-sensitive optical coherence tomography (PS-OCT). Eyes of patients with subretinal fibrosis secondary to nAMD were included in this case series. All patients underwent a complete ophthalmic examination to clearly identify advanced nAMD lesions with fibrosis. Examinations of PS-OCT were performed using a novel system with an integrated eye tracker. Areas of fibrosis in PS-OCT, automatically segmented using a custom-built algorithm, were compared with conventional imaging modalities including spectral-domain OCT, fluorescein angiography, and color fundus photography in their potential to visualize fibrosis in nAMD. Fifteen eyes of 15 consecutive patients were included. In polarization-sensitive OCT B-scans, a distinct "column-like" pattern was observed in averaged axis orientation images. En face analysis provided a precise mapping of the fibrotic scar component. Fibrous tissue was selectively identified by PS-OCT based on birefringence in all lesions, whereas in SD-OCT, subretinal hyperreflective material (SHRM) could not be further classified into scar tissue, fibrovascular material, or other AMD-specific material. Based on simultaneous polarization analyses in PS-OCT, the level of RPE alteration could be evaluated as well, showing thinning and loss of RPE associated with subretinal fibrosis. Using PS-OCT, subretinal fibrosis can be identified as an intrinsically birefringent structure and can be segmented based solely on tissue-specific contrast. Polarization-sensitive OCT offers a unique method to identify clinically relevant components of SHRM (i.e., neovascular tissue versus fibrous tissue) and therefore allows for an optimized disease management and evaluation of therapeutic strategies.

Osteoarthritis is a prevalent medical condition that presents a diagnostic and therapeutic challenge to physicians today because of the inability to assess the integrity of the articular cartilage early in the disease. Polarization sensitive optical coherence tomography (PS-OCT) is a high resolution, non-contact imaging modality that provides cross-sectional images with additional information regarding the integrity of the collagen matrix. Using PS-OCT to image provides information regarding thickness of the articular cartilage and gives an index of biochemical changes based on alterations in optical properties (i.e. birefringence) of the tissue. We demonstrate initial experiments performed on specimens collected following total knee replacement surgery. Articular cartilage was imaged using a 1310 nm PS-OCT system where both intensity and phase images were acquired. PS-OCT images were compared with histology, and the changes in tissue optical properties were characterized. Analysis of the intensity images demonstrates differences between healthy and diseased cartilage surface and thickness. Phase maps of the tissue demonstrated distinct differences between healthy and diseased tissue. PS-OCT was able to image a gradual loss of birefringence as the tissue became more diseased. In this way, determining the rate of change of the phase provides a quantitative measure of pathology. Thus, imaging and evaluation of osteoarthritis using PS-OCT can be a useful means of quantitative assessment of the disease.

Polarization sensitive optical coherence tomography (PS-OCT) was used to measure and image retardation and birefringent axis orientation of in vitro human cornea. We used a two-channel PS-OCT system employing a phse sensitive recording of the interferometric signals in two orthogonal polarization channels. Using an algorithm based on a Hilbert transform, it is possible to calculate the retardation and the slow axis orientation of the sample with only one A-scan per transversal measurement location. While the retardation information is encoded in the amplitude ratio of the two interferometric signals, the axis orientation is encoded entirely in their phase difference. We present maps of retardation and slow axis distribution of normal and pathologic human cornea in longitudinal cross sections and en face images, obtained at the back sruface of the cornea. The results can be explained by a birefringence model based on stacked collagen fibril lamellae of different orientations.

We present a novel time-domain polarization sensitive optical coherence tomography configuration operating at 830 nm, equipped with multichannel acousto-optic deflectors (AOD)s. The system can be used to simultaneously acquire interference information from multiple polarization-sensitive channels and to enable measurement and imaging of backscattered intensity, birefringence, and fast optic axis orientation. The system is employed here to demonstrate polarization sensitive imaging of a thermally damaged muscle tissue.

A polarization sensitive optical coherence tomography (PS-OCT) instrument was used to investigate the retinal pigment epithelium (RPE). The instrument uses the polarization properties of light to record backscattered intensity, retardation and fast axis orientation simultaneously and needs only one measurement per sample location to retrieve these parameters. The polarization state of light backscattered from within the RPE was found to be random. This can be observed in PS-OCT images by random retardation and axis orientation values within the RPE layer. In diseased eyes where the normal retinal structure is corrupted (e.g. RPE atrophy, RPE detachment) the localization of the RPE within OCT images which do not provide polarization information (standard OCT) is rather difficult. Since the RPE is the only structure within the retina to cause this polarization scrambling, PS-OCT can be used for contrast enhancement and enables the exact localization of the RPE in these pathologies. Therefore it is possible to determine if the RPE is still preserved in regions of interest. Furthermore, in patients with RPE atrophy an enhanced penetration depth into the choroid and even into the sclera was observed. Because of birefringence introduced by the sclera the border between choroid and sclera could easily be determined.

Polarization-sensitive optical coherence tomography (PS-OCT) for retinal imaging has been developed with time-domain or spectral-domain OCT using broadband light sources at 800-nm band. Recent studies suggested that a 1 μm band could achieve higher penetration for imaging the posterior segment of the eye. In this presentation, we demonstrate fiber-based swept-source PS-OCT at 1 μm wavelength. Birefringence of the posterior eye segment is measured with a single depth scan by using the method of continuous polarization modulation. To our knowledge, this is the first time to show the phase retardation image of the posterior eye segment at 1 μm wavelength.

Previous studies have demonstrated that optical coherence tomography (OCT) could be used to delineate alterations in the microstructure of cartilage, and have suggested that changes in the polarization state of light as detected by OCT could provide information on the birefringence properties of articular cartilage as influenced by disease. In this study we have used both OCT and polarization sensitive optical coherence tomography (PS-OCT) technologies to evaluate normal and abnormal bovine articular cartilage according to established structural, organizational, and birefringent characteristics of degenerative joint disease (DJD) in order to determine if this technology can be used to differentiate various stages of DJD as a minimally invasive imaging tool. Fresh bovine femoral-tibial joints were obtained from an abattoir, and 45 cartilage specimens were harvested from 8 tibial plateaus. Whole ex vivo specimens of normal and degenerative articular cartilage were imaged by both OCT and PS-OCT, then fixed and processed for histological evaluation. OCT/PS-OCT images and corresponding histology sections of each specimen were scored according to a modified Mankin structural grading scale and compared. OCT and PS-OCT imaging allowed structural evaluation of intact articular cartilage along a 6 mm surface length to a depth of 2 mm with a transverse resolution of 12 microm and an axial resolution of 10 microm. The OCT and PS-OCT images demonstrated characteristic alterations in the structure of articular cartilage with a high correlation to histological evaluation (kappa = 0.776). The OCT images were able to demonstrate early to advanced structural changes of articular cartilage while the optical phase retardation images obtained by PS-OCT imaging were able to discriminate areas where disorganization of the cartilage matrix was present, however, these characteristics are much different than those reported where OCT images alone were used to characterize tissue birefringence. No evidence of differences in OCT or PS-OCT images were detected between specimens of similar structural characteristics where proteoglycan was judged present or absent by safranin-O Fast Green staining. The combined use of OCT and PS-OCT technologies to obtain images from a single system is able to demonstrate and discriminate between characteristics of very early stages of surface irregularities not previously reported for OCT imaging, to deep clefts and collagen matrix disorganization for tissue at depths of up to 2 mm with good correlation to histology. PS-OCT and accumulated optical phase retardation images of articular cartilage as constructed from alterations in Stokes vector parameters appear to give a valuable but different assessment of alterations in tissue birefringence and organization than have been reported for OCT images obtained with the use of polarized or non-polarized light sources. This is the first time that alterations in the polarization state of light reflected from within the tissue have been demonstrated to be consistent with changes observed in the orientation and organization of the collagen matrix in advanced stages of DJD. The degree of phase transformation of light reflected from within the tissue as determined by PS-OCT imaging does not appear to be altered by the presence or absence of proteoglycan.