Solução Ambiental Assessoria e Consultoria

O estudo teve como objetivo avaliar a ocorrência sazonal do pulgão Myzus persicae e da formiga Solenopsis sp. na cultura de berinjela, cultivar Napoli, desde o transplante até o final do desenvolvimento da cultura, na região de Ilha Solteira – SP. O experimento foi conduzido na Fazenda de Ensino, Pesquisa e Extensão da FEIS/UNESP, utilizando 180 mudas transplantadas para o campo. As avaliações foram realizadas semanalmente, a partir de 33 dias após o transplante, com amostragem aleatória de 20 plantas por semana. Cada planta foi dividida em terços (superior, médio e inferior) para contagem de pulgões e formigas, sendo os pulgões quantificados em áreas padronizadas da face inferior das folhas e as formigas contadas em folhas e ramos. Os resultados mostraram que a maior população de Myzus persicae ocorreu entre 54 e 89 dias após o transplante, com pico aos 68 dias (média de 9,7 pulgões por 4 cm²), predominando no terço inferior das plantas. As condições climáticas nesse período variaram entre 19 e 29°C e 47 a 91% de umidade relativa. Para Solenopsis sp., os maiores picos populacionais ocorreram entre 54 e 75 dias e entre 126 e 141 dias após o transplante, com maior densidade aos 126 dias (média de 54 formigas por planta), também concentradas no terço inferior. No início do ciclo da cultura, as formigas atacaram os ponteiros e formaram ninhos no colo das plantas, abrindo galerias no caule e nos ramos. Na fase reprodutiva, atacaram os frutos, causando deformações e prejuízos comerciais. Observou-se ainda correlação entre a presença das formigas e as excreções açucaradas (honeydew) produzidas pelos pulgões, que servem de alimento para as formigas. Conclui-se que tanto o pulgão quanto a formiga apresentam maior incidência no terço inferior das plantas, com períodos críticos distintos ao longo do ciclo da cultura, e que existe associação ecológica entre as duas espécies, potencializando os danos à berinjela.

Bone regeneration remains a clinical challenge, particularly in critical-size defects, motivating the investigation of biomaterials and adjuvant therapies that may support tissue repair. This experimental study evaluated bone healing in critical-size calvarial defects created in rats, using different combinations of regenerative strategies, including heterologous fibrin biopolymer gel, bovine cortical bone biological membrane, and photobiomodulation. Standardized 5.0 mm calvarial defects were surgically created in sixty Wistar rats, which were randomly allocated into six experimental groups according to the filling material and the application or absence of photobiomodulation. The treatments included clot alone, fibrin biopolymer gel, biological membrane, photobiomodulation, or their respective combinations. Animals were euthanized at 14 or 42 days, and bone repair was evaluated by histomorphometric analysis. At 14 days, differences in the extent of newly formed bone were observed among the experimental groups, with higher bone formation values detected in groups receiving combined treatments and lower values in groups treated with fewer regenerative components. At 42 days, all groups showed progression of bone repair, with greater bone formation observed in groups in which a biological membrane was used, regardless of photobiomodulation. Overall, the findings indicate that the association of different regenerative approaches was related to variations in bone repair patterns over time, suggesting that photobiomodulation, when applied in combination with biomaterials, may be associated with differences in early bone healing, without implying a direct causal effect.

The present study aimed to evaluate the cardiopulmonary risks associated with electronic cigarette use in young people aged 15 to 24, through an integrative review of the literature. The research was conducted in databases such as SciELO, PubMed, Lilacs, and VHL (Virtual Health Library), using portuguese and english descriptors and the PICO strategy, with inclusion and exclusion criteria based on the PRISMA protocol. A total of 457 articles were initially identified, of which 17 met the established criteria. The results demonstrated that electronic cigarette use is associated with significant cardiovascular and pulmonary changes. Among the cardiovascular risks, the following stand out: elevated blood pressure, increased sympathetic activity, oxidative stress, inflammation, endothelial dysfunction, arterial stiffness, atherosclerosis, arrhythmias, angina, and acute myocardial infarction. Regarding pulmonary risks, a higher incidence of spontaneous pneumothorax, exacerbation of asthma and bronchitis, chronic obstructive pulmonary disease (COPD), and E-cigarette or Vaping product use-Associated Lung Injury (EVALI) were observed. It is concluded that electronic cigarettes represent a significant risk factor for the health of young people, potentially leading to chronic and life-threatening conditions. Thus, the intensification of preventive, educational, and regulatory measures is recommended, as well as the development of longitudinal studies that evaluate the long-term effects of using these devices.

A pandemia de COVID-19 afetou de forma diferenciada populações ao redor do mundo, tendo seus impactos associados a diversos fatores econômicos e sociais. Um dos efeitos da pandemia foi a redução das taxas de natalidade, um fenômeno identificado por todo o mundo e chamado de baby bust. Foram utilizados dados públicos de natalidade e do Google Mobility Reports, além da tipologia urbana x rural do IBGE para comparar os efeitos do baby bust entre os municípios com diferentes graus de urbanização no estado do Espírito Santo por meio de análises de variância. Nos municípios classificados como “Urbano”, o número médio de nascimento por ano durante o período pré-isolacional (antes de 2020) foi de 39.712, durante o período transicional (ano de 2020) foi de 37.723 e durante o período pós-isolacional (após 2020) foi de 36.165, sendo que a diferença entre os períodos foi significativa, diferentemente do observado entre os municípios com menor grau de urbanização. Os motivos desta diferença são discutidos em detalhes levando em conta aspectos particulares do cenário pandêmico brasileiro, chamando atenção para a importância do planejamento de políticas públicas que levem em consideração as diferenças sociais e econômicas entre os municípios do país para, desta forma, mitigar os efeitos de futuras pandemias nas taxas de natalidade.

Disclosure: Y.D. Moura: None. M.F. Fernandes Martins: None. B.G. Garcia: None. S. Bertolini: None. G.U. Durão: None. K.C. Queiroz Barreto: None. L. Oliveira Risso da Costa: None. A. Cardoso Rodrigues: None. R. Rodrigues Barroso de Souza: None. L.F. Siqueira Ribeiro: None. V. Cândido Bianqui: None. A. Carvalho de Oliveira Guilherme: None.INTRODUCTION Idiopathic short stature (ISS), as defined by the Brazilian Society of Pediatrics, refers to a height more than 2 standard deviations (SD) below the mean for age, sex, and population, with no apparent organic causes, normal body proportions, and adequate nutrition. According to Pfizer, major risk factors include familial short stature (FSS) and constitutional delay of growth and puberty (CDGP). Clinical signs involve height below the average for age and sex and a predicted final height lower than that of the parents. Diagnosis is based on exclusion criteria, requiring laboratory tests and imaging for bone assessment. Among therapeutic approaches, aromatase inhibitors (AIs) have emerged as an option for boys with ISS. These drugs inhibit the conversion of androgens to estrogens, delaying epiphyseal maturation and extending bone growth. AI use can increase adult height without compromising bone health or pubertal development. Compared to GnRH analogs, which induce temporary hypogonadism, AIs offer a better safety profile, allowing normal pubertal progression. Thus, they represent a promising strategy to optimize growth in this population. METHODOLOGY A systematic search was conducted in PubMed, Cochrane Library, and Scopus to identify studies on AI use in the treatment of ISS in children and adolescents. Included studies comprised randomized clinical trials, observational studies, and systematic reviews published in the last 5 to 10 years in Portuguese, English, or Spanish. Inclusion criteria covered studies evaluating final height, growth velocity, bone maturation, and adverse effects. Research with a high risk of bias or insufficient samples was excluded. RESULTS Studies indicate that AI use, alone or combined with growth hormone (GH), can enhance final height potential and lean body mass with a favorable safety profile. The inhibition of estrogen biosynthesis prolongs longitudinal growth due to its role in bone maturation and epiphyseal closure. Vitamin D deficiency, commonly observed in ISS patients, exacerbates bone fragility, increasing fracture risk. Calcitriol supplementation has shown improvements in bone parameters and reductions in elevated PTH levels. However, improper vitamin D use may lead to complications such as vascular calcification and hyperphosphatemia. Strict monitoring of calcium, phosphorus, and PTH levels is essential to prevent adverse effects. CONCLUSIONISS diagnosis requires excluding other pathologies. AIs have proven effective in delaying bone maturation and promoting growth without impairing pubertal development. However, individualized treatment and careful monitoring of bone parameters are crucial to optimizing outcomes and ensuring long-term safety. Treatment with vitamin C must be strictly controlled, as excess can lead to complications, therefore, new studies are necessary to prove its effectiveness.Presentation: Saturday, July 12, 2025

Cannabidiol (CBD) is a non-psychotropic compound found in plants of the Cannabis genus, extensively studied for its therapeutic potential. Research has shown that CBD possesses anti-inflammatory, antioxidant, and regenerative properties, and may contribute to the recovery of neural and bone tissues. In light of the aging population and the resulting rise in neurodegenerative and osteodegenerative conditions, exploring novel therapeutic strategies that promote cellular regeneration is increasingly important. This review aims to compile and critically analyze key studies published in recent decades regarding the effects of CBD on the regeneration of the central and peripheral nervous systems, as well as bone tissue. Findings from in vivo studies indicate that CBD can attenuate inflammatory responses, inhibit oxidative stress, and modulate cellular pathways involved in tissue repair, thereby supporting neuronal and bone regeneration. Moreover, evidence suggests that CBD may protect cells from structural damage, enhancing the functional recovery of affected tissues. Despite scientific advances highlighting cannabidiol as a promising agent for bone and nerve regeneration, its therapeutic application still faces significant limitations. The primary challenge lies in the lack of robust clinical trials in humans, as most existing evidence is derived from in vitro and in vivo studies, making it difficult to confirm its efficacy and safety in clinical contexts. Additionally, CBD’s low bioavailability—due to first-pass hepatic metabolism—hinders dose standardization and reduces the predictability of therapeutic outcomes. Compounding these issues are regulatory constraints and the persistent social stigma surrounding cannabis-derived compounds, which further impede their integration and acceptance in regenerative medicine. Therefore, future research is essential to validate the therapeutic benefits of CBD and to establish its clinical applicability in treating neurological and bone disorders.

Introdução: A osteoartrite de joelho é uma doença degenerativa crônica caracterizada pela destruição da cartilagem, dor progressiva, rigidez e limitação funcional. Sua prevalência aumenta com a idade e está associada a elevado impacto socioeconômico, incluindo incapacidade e aposentadorias precoces. O manejo adequado é essencial para reduzir sintomas e melhorar a qualidade de vida. Métodos: Foi realizada uma revisão sistemática de diretrizes clínicas nacionais e internacionais, obtidas em bases como MEDLINE, PubMed, NICE, International Guideline Library e Scopus, sem restrição temporal. Foram incluídas diretrizes em inglês, português e espanhol, com informações sobre diagnóstico e tratamento da osteoartrite de joelho. A qualidade metodológica foi avaliada pelo instrumento AGREE II, considerando escopo, rigor, clareza, aplicabilidade e independência editorial. Resultados: Foram analisadas 14 diretrizes publicadas entre 2011 e 2022, provenientes de países como Canadá, Brasil, Espanha, Estados Unidos, China e Itália. Observou-se consenso na ênfase ao tratamento não farmacológico, principalmente exercícios físicos regulares supervisionados, associados à perda de peso em pacientes obesos. Em relação ao tratamento farmacológico, destacam-se anti-inflamatórios não esteroidais tópicos como primeira opção e uso criterioso de AINEs orais e corticosteroides intra-articulares para alívio temporário da dor. O ácido hialurônico apresenta benefício mais prolongado, embora de início tardio. Terapias emergentes, como plasma rico em plaquetas (PRP), mostram potencial, mas carecem de maior evidência. Intervenções cirúrgicas, como osteotomia e artroplastia total do joelho, são reservadas para casos refratários, sendo a cirurgia artroscópica de benefício limitado e controverso. Conclusão: As diretrizes reforçam que o tratamento da osteoartrite de joelho deve priorizar intervenções não farmacológicas, com destaque para exercícios físicos e controle de peso. Abordagens farmacológicas e cirúrgicas devem ser individualizadas e utilizadas de forma escalonada. Novas terapias biológicas, como PRP, representam perspectivas futuras promissoras, mas ainda não substituem os tratamentos convencionais.

Abstract This study investigated the impact of heat stress on serum proteomics, laying performance, and egg quality in aged laying hens. Forty-eight 75-week-old Lohmann White hens were divided into two groups and subjected to either a comfort environment (26 °C) or heat stress (35 °C) for 28 days. Serum samples were collected on days 1 and 28 for proteomic analysis, while egg production and quality parameters were assessed daily throughout the experiment. Heat stress reduced egg production (11%), egg weight (16%), shell strength (30%), shell thickness (13.5%), and albumen height (12%). Proteomic analysis revealed the expression of proteins that bind to the heat shock proteins Hsp70 and Hsc70, exclusively in heat-stressed laying hens. Proteins related to coagulation rate were upregulated and when compared with field observations may indicate possible Disseminated Intravascular Coagulation (DIC). Apolipoprotein A and apovitelenin-1 were associated with decreased egg production and quality under heat stress.

Endothelial inflammation and atherosclerosis remain leading drivers of cardiovascular disease, yet the post-transcriptional regulators orchestrating these events are not yet completely understood. In this review, we analyse recent preclinical and clinical studies to dissect microRNA (miRNA)-mediated control of vascular endothelial biology. We describe how miR-181b-5p and miR-223 modulate NLRP3 inflammasome activation and pyroptosis, how miR-615-5p, miR-138, and miR-133a coordinate endothelial nitric oxide synthase (eNOS) activity and nitric oxide bioavailability, and how miR-33a/b, miR-150, and miR-342-3p influence lipid efflux and foam-cell formation in atherogenesis. We also discuss miRNA signatures that correlate with endothelial dysfunction in human cohorts. By integrating mechanistic pathways with emerging biomarker data, this study underscores the relevance of miRNAs as both diagnostic and potential targets in vascular diseases.