White cell retention compared with platelet retention in the filterometer: controls and abnormal states

The Effect of Uteroferrin and Recombinant GM-CSF on Hematopoietic Parameters in Normal Female Pigs (Sus scrofa)

The acute effects of dihydrocapsaicin (DHC) and capsaicin (CAP) on the number of white blood cells (WBCs), neutrophils, eosinophils, basophils, monocytes, lymphocytes, T lymphocytes, B lymphocytes and NK cells, and serum corticosterone levels were studied in rats. Male 7-wk-old SD rats were divided into DHC (3.0 mg/kg BW), CAP (3.0 mg/kg BW) and control (CON) groups. The number of total WBCs was 1.30-1.42 times significantly higher in the DHC group than in the CON group at 6-12 h. The number of neutrophils was 1.62 times significantly higher in the DHC group than in the CON group at 12 h. The number of total WBCs and neutrophils, however, showed no significant changes between the CAP and CON groups. The number of lymphocytes was 0.61 and 0.70 times significantly lower in the DHC and CAP groups than in the CON group at 3 h. The number of T lymphocytes and B lymphocytes was 0.74 and 0.54 times lower in the DHC group than in the CON group, respectively. CAP, however, did not significantly change the number of T lymphocytes or B lymphocytes. No significant changes in the number of NK cells were observed among the three groups. CAP and DHC did not change the number of monocytes, eosinophils or basophils. No significant changes of the serum corticosterone levels were observed among the three groups. In conclusion, capsaicinoids decreased the number of acquired immunity cells, and increased the number of total WBCs and neutrophils without changing the number of monocytes, eosinophils or basophils. The magnitude of these effects was relatively higher in DHC than in CAP.

The aim of this work was to determine the role of intraperitoneally-administered vitamin E and selenium on the biochemical and haematological parameters in the blood of rats. Thirty-two adult male Wistar rats were used in this study. All rats were randomly divided into four groups. The first group was used as the control. The second group was intraperitoneally administered with vitamin E (+/-(-)alpha-tocopheroryl acetate, 10 mg day-1), the third group with Se (Na2SeO3 0.2 mg over a day), and the fourth group with vitamin E and Se (vitamin E 10 mg + Na2SeO3 0.2 mg over a day). This administration was done for 5 weeks. Blood samples were taken from animals at the end of the dosage period and biochemical parameters in serum samples and haematological parameters in total blood were determined. The levels of total cholesterol (p < 0.01) and number of white blood cell (p < 0.001) in blood were significantly higher in the vitamin E group than in the control group. The levels of ALP, total cholesterol (p < 0.01) and number of white blood cells (p < 0.01) in blood were significantly higher in the selenium group than in the controls. The levels of glucose (p < 0.05), ALP (p < 0.01), total cholesterol (p < 0.001) and number of white blood cells (p < 0.01) were higher in the vitamin E and selenium combined group than in the controls. Other parameters considered within this trial (ALT, LDH, creatinine, albumin, total protein, amylase, creatine kinase, HDL triglycerides, total lipid, sodium, chloride, uric acids, red blood cell, haemoglobin, packed cell volume, MCV, MCH, MCHC) did not show statistically significant differences between the control and injected groups. The results indicated that blood glucose and total cholesterol levels, ALP activity and white blood cell counts were significantly increased by intraperitoneal administration of vitamin E and selenium in rats.

Dengue hemorrhagic fever (DHF) is a public health problem in Indonesia whose number of cases continues to increase and its spread is increasingly widespread.Tests used to diagnose dengue fever include a white blood cell count test.White blood cells are cells that play a role in the body's defense system. The reason is, white blood cells help fight infections in the body, such as viruses, bacteria, parasites and fungi. In patients infected with dengue fever, the white blood cell count is usually normal but then decreases (leukopenia) and neutropenia becomes dominant. Thisresearch was conducted from March to April 2023.The aim of this study was to measure the number of white blood cells in dengue fever patients.This type of research is descriptive. The research was carried out using an automatic analysis method (blood analysis) in the laboratory ofKlinik Dr.Trisna Garut, using samples from 30 dengue fever patients who were tested. In this study, the number of white blood cells in dengue fever patients at Klinik Dr.Trisna Garut was 14 (46.7%).Wich is Normal And the white blood cell count results of dengue fever patients at Klinik Dr.Tisna Garut General Hospital decreased by 16 (54.3%).

Thirteen patients with chronic granulocytic leukemia were given 28 separate irradiations to the spleen for the treatment of their disease. The radiation was given as a single dose that ranged between 100 and 700 rads. The changes in the circulating white blood cell (WBC) count could be represented by the expression $N=B_{0}+S_{0}(e^{-\beta t})$ where N is the number of WBC per mm3 of blood at day t; S0 is the number of WBC, at time zero, originating from the spleen; and B0 is the number of WBC stemming from nonirradiated parts of the body, mostly bone marrow. The disappearance rate β, determined by an indirect method, indicated that S0 cells were leaving the blood with a half-time of 4.5 days. The number of S0 cells could be calculated a posteriori by the expression $S_{0}=(N_{0}-N)/(1-e^{-\beta t})$, where N0 is the number of WBC before spleen irradiation and N the number of WBC at t days after irradiation.

The purpose of this study is to see how low-power red laser helium-neon and diode lasers with wavelengths of 632.8 nm and 650 nm, respectively, affect some human blood sample factors like viscosity HCT, Platelets PLT, White blood cells WBC, white blood lymphocytes LYM, neutrophilic white blood cells NEUT, red blood cells RBC, and Erythrocyte Sedimentation Rate ESR. Blood samples were collected from healthy people and placed in EDTA-contained tubes, blood tests were performed on control samples in a blood analyzer, and the samples were divided into two parts and split into two equal tubes to be irradiated with diode and helium-neon lasers for 15 minutes. Using a blood analyzer, measurements were taken immediately after irradiation. The results of this analysis indicated a decrease in the number of white blood cells (WBC), red blood cells (RBC), and blood viscosity (HCT), as well as a significant rise in the number of neutrophils (NEUT) and platelets (PLT) and increase in the Erythrocyte Sedimentation Rate ESR after exposure to both helium-neon and diode lasers. This suggests that It is possible to make recommendations, a low-power laser could be used to stimulate blood cells so a laser could be used to treat blood viscosity. Because of the increasing concentrations of free intracellular Calcium ions and the action of laser on the plasma composition, it is acceptable to suggest that laser irradiation can lower the number of white blood cells (WBC), red blood cells (RBC), and the reason of that is because of a mechanical change in the blood such as a change in the pores of the surfaces and their fixation.

A leukocyte and erythrocyte adhesiveness/aggregation test to reveal the presence of smoldering inflammation and risk factors for atherosclerosis

Since blood cells count is the most important indicator of animals' physiological status, we investigated the effects of age, litter size, and multiple paternity on the total number of white blood cells, the number of their individual types (lymphocytes, neutrophils, eosinophils, monocytes), and the ratio of neutrophils to lymphocytes in domestic cat during early postnatal ontogenesis. The study was conducted on kittens living in outdoor conditions, aged from birth to 6 months. The number of white blood cells was evaluated using a hemoanalyzer, the leukocyte formula and the proportion of cell types were determined manually from blood smears. Age significantly affected the number of leukocytes in the first 3 months of kittens' age. The number and proportion of neutrophils were the highest after birth and gradually decreased during the first month. Lymphocytes number, on the contrary, increased during this period. Monocytes and eosinophils increased in number in the first 2 months. The litter size affected the number of leukocytes and neutrophils in the first 2 months of life, their number being significantly higher in kittens from the small litters than from the large ones. In kittens from the litters with multiple paternity, the number of leukocytes and the proportion of neutrophils was higher than in litters from a single male. Thus, age, litter size and type of paternity may affect the hematological indices in domestic cats, which must be taken into account during the estimation of the health status of kittens in domestic and wild cats.

Determination of the Volume of HPC, Apheresis Products Based on Weight in Grams

Objective To observe the clinical efficacy and safety of Reduning injection combined with piperacillin sulbactam in the treatment of community acquired pneumonia(CAP). Methods 122 cases with CAP were randomly divided into treatment group and control group by digital table method.The two groups were given piperacillin sodium and sulbactam sodium 3.75g, once every 12 hours, course 7-10d.The treatment group was treated with Reduning injection, 20ml/d treatment for 7-10d.The clinical symptoms of the two groups before and after treatment, the total number of white blood cells(WBC) and C reactive protein(CRP) to resume normal time were observed. Results The effective rate was 94.91% in the treatment group and 80% in the control group, the difference between the two groups was statistically significant(χ2=10.286, P<0.05). The treatment group was significantly shorter than the control group in controlling fever and cough symptoms(all P<0.05).3 months after treatment, the WBC and CRP recovery to the normal rate of the two groups had statistically significant differences (χ2=2.880, 3.926, all P<0.05). No obvious toxic side effects and adverse reactions were observed in the two groups. Conclusion Reduning injection combined with piperacillin sodium and sulbactam sodium in the treatment of CAP can alleviate the clinical symptoms of fever.This method has good clinical curative effect and high safety.It can be used as an effective method for treatment of CAP. Key words: Pneumonia; Community-acquired infections; Reduning injection; Piperacillin sodium and sulbactam sodium

In this work, an integrated biosensor consisting of spiral microfluidic array and microwave sensors is proposed for simultaneous separation and detection of cells. The biosensor integrated by plasma processing technology is fabricated by soft lithography and glass‐based IC process, which has the advantages of simple preparation, low cost, and reliable structure. In the field of clinical medicine, Escherichia coli ( E. coli ) is the causative agent of urinary tract infections, which leads to an increase in the number of white blood cells (WBCs) present in the urine. Different concentrations of E. coli and WBCs mixed solution are configured to perform biological cell experiments and the capability of the biosensor in separating and detecting WBCs is verified. Interdigital capacitors (IDCs) and split ring resonators (SRRs) are employed to detect the WBCs obtained by microfluidic array separation. The microfluidic array exhibits a WBC collection rate of 92.7%. The capacitance of the IDC and the resonant amplitude of the SRR exhibit a decrease of 51.36 pF and 0.34 dB, which demonstrates a satisfactory linearity of 0.96 and 0.98, respectively. Consequently, the integrated biosensor used to simultaneously separate and detect WBCs has the potential for the early diagnosis of urinary tract infections in clinical medicine.

Objective The diagnostic value of neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), and platelet-to-lymphocyte ratio (PLR) in predicting fracture-related infection (FRI) in tibia fracture patients remains to be explored. Methods A retrospective controlled study was carried out with 170 tibia FRI patients and 162 control subjects. The following information was evaluated at admission: age, gender, clinical features, number of white blood cells (WBCs), neutrophils, lymphocytes, monocytes, red blood cells (RBCs), platelets, level of hemoglobin, C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR), as well as NLR, MLR, and PLR. Results The number of lymphocytes, RBCs, and platelets in the FRI group was higher than those in the control group, while the number of neutrophils and ESR level was lower (P < 0.05). The level of NLR and MLR was significantly lower in patients with tibia FRI than in control subjects (P < 0.05). Both indicators were positively correlated with WBCs, CRP level, and ESR level (P < 0.001). The results of logistic regression analysis showed that five variables including NLR, MLR, platelets, fracture pattern (closed or open fracture), and site pattern (single or multiple site) were used to construct the FRI risk predictor. The ROC curve analysis result showed that FRI risk predictor yielded the highest AUC, with a sensitivity of 91.2% and a specificity of 90.1%, and made the distinction efficiently between tibia FRI patients and non-FRI patients. Conclusion NLR and MLR were decreased in tibia FRI patients compared to non-FRI patients. Both indicators had a positive correlation with WBCs, CRP level, and ESR level. FRI risk predictor constructed based on five variables including NLR and MLR had a high diagnostic value for tibia FRI.

Objective To investigate the effect of enteral microecopharmaceutics nutrition supplement (EMN) on incidence of ventilator associated pneumonia complications, inflammatory responses, and prognoses in patients with severe traumatic brain injury (sTBI) undergoing ventilator therapy. Methods From January 2016 to September 2017, 70 patients with sTBI were randomly divided into an experimental group (EMN supplement, n=35) and a control group (n=35). Early enteral nutrition was given to patients from both groups, and bifidobacterium viable capsule was given to patients from the experimental group. Glasgow Coma Scale (GCS) scores, acute physiological and chronic health evaluation (APACHE) II scores, number of white blood cells (WBC), proportion of neutrophil (N), and serum levels of procalcitonin (PCT) and C-reactive protein (CRP) were compared between the two groups on admission and 14 d after treatment. The incidence of VAP and clinical pulmonary infection scale (CPIS) scores, pulmonary function, mechanical ventilation time and hospital stays were compared 14 d after treatment. Results The incidence of VAP in the experimental group (n=17, 51%) 14 d after EMN supplement was significantly lower as compared with that in the control group (n=22, 63%, P<0.05). Meanwhile, VAP patients from the experimental group had significantly lower CPIS scores as compared with patients from control group (P<0.05). Ventilator weaning was achieved in 29 patients from experimental group (83%) and 24 patients from control group (69%), with significant difference (P<0.05); ventilator weaning patients from the experimental group had significantly improved lung function as compared with ventilator weaning patients from control group (P<0.05). WBC number, N proportion, and serum PCT and CRP levels in the experimental group were significantly smaller/decreased as compared with those in the control group (P<0.05). As compared with patients from control group, patients from the experimental group had significantly higher GCS scores (8.35±0.51 vs. 9.48±0.48, P<0.05) and significantly lower APACHE II scores (12.58±0.78 vs. 14.68±0.97, P<0.05). Conclusion EMN supplement can effectively reduce the VAP incidence, alleviate the degree of infection and inflammatory response in patients with sTBI undergoing ventilator therapy, therefore, improve the prognosis; this method is worth of promotion. Key words: Traumatic brain injury; Enteral microecopharmaceutics nutrition; Ventilator associated pneumonia; Inflammatory response

BackgroundElevated total white blood cell (WBC) count is associated with an increased risk of coronary heart disease and death. Aerobic exercise is associated with lower total WBC, neutrophil, and monocyte counts. However, no studies have evaluated the effect of the amount of aerobic exercise (dose) on total WBC and WBC subfraction counts.PurposeTo examine the effects of 3 different doses of aerobic exercise on changes in total WBC and WBC subfraction counts and independent effects of changes in fitness, adiposity, markers of inflammation (IL-6, TNF-α, C-reactive protein), fasting glucose metabolism, and adiponectin.MethodsData from 390 sedentary, overweight/obese postmenopausal women from the DREW study were used in these analyses. Women were randomized to a non-exercise control group or one of 3 exercise groups: energy expenditure of 4, 8, or 12 kcal kg−1⋅week−1 (KKW) for 6 months at an intensity of 50% VO2peak.ResultsA dose-dependent decrease in total WBC counts (trend P = 0.002) was observed with a significant decrease in the 12KKW group (−163.1±140.0 cells/µL; mean±95%CI) compared with the control (138.6±144.7 cells/µL). A similar response was seen in the neutrophil subfraction (trend P = 0.001) with a significant decrease in the 12KKW group (−152.6±115.1 cells/µL) compared with both the control and 4KKW groups (96.4±119.0 and 21.9±95.3 cells/µL, respectively) and in the 8KKW group (−102.4±125.0 cells/µL) compared with the control. When divided into high/low baseline WBC categories (median split), a dose-dependent decrease in both total WBCs (P = 0.003) and neutrophils (P<0.001) was observed in women with high baseline WBC counts. The effects of exercise dose on total WBC and neutrophil counts persisted after accounting for significant independent effects of change in waist circumference and IL-6.ConclusionAerobic exercise training reduces total WBC and neutrophil counts, in a dose-dependent manner, in overweight/obese postmenopausal women and is especially beneficial for those with systemic low grade inflammation.Clinical Trials Identifier: NCT00011193

Objective To investigate the relationship between vitamin D, fibrinogen, white blood cell count and hypertension, find out the correlation of vitamin D and fibrinogen. Methods Oct.2010 to Oct.2012 hospitalised 326 hypertensive patients and 218 healthy cases were involved. To analysis the differences of fibrinogen, vitamin D and the number of white blood cells status. Then research the correlation of vitamin D and fibrinogen in hypertension group. Results Compared with the hypertension group and the non-high-blood group, vitamin D, fibrinogen, the number of white blood cells, lymphocytes, neutrophils and lipids, the difference was statistically significant (P &lt; 0.05). To analysis the multiple factors regression, for hypertension, vitamin D is protective factor (B value = 0.186, OR value = 0.83, 95% CI0.795–0.869), but neutrophils, fibrinogen, low density lipoprotein are risk factors (B value were 0.761, 0.173, 0.987, OR value were 2.141, 1.189, 2.128, 95% CI1.445–3.1731, 1.14 1.908, 1.84 3.971). Hypertension group vitamin D and fibrinogen have negative correlation (r = 0.152, P = 0.011). Conclusions The vitamin D level of hypertension group was higher and the fibrinogen level was lower than the non-hypertensive group. For hypertension, vitamin D is protective factor, while neutrophils and fibrinogen are risk factors. Vitamin D and fibrinogen were negatively correlated in hypertension patients.